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| 1 | Pliocene charcoals from Shanxi Province of China and their application to studies of prehistoric wildfires显示文摘Charcoals collected from the middle-late Pliocene sediments of the Taigu Basin,Shanxi Province,China,have been identified as Ulmus sp.(Ulmaceae),Prunus sp.,Maloidoxylon sp.(Rosaceae),and Maclura sp.(Moraceae).These taxa,along with the previously known fossils,indicate the occurrence of temperate climate and local wildfire at that time.Charcoals of trees and/or shrubs and the morphological changes of these charcoals demonstrate that crown fires and surface fires occurred in the Taigu Basin during the middle-late Pliocene. | CHENG YeMing JIANG XiaoMei LI ChengSen WANG YuFei | 2011 | Science China Earth Sciences2011，54，4： | 1 |
| 2 | Defective EMC1 drives abnormal retinal angiogenesis via Wnt/β-catenin signaling and may be associated with the pathogenesis of familial exudative vitreoretinopathy显示文摘Endoplasmic reticulum(ER)membrane protein complex(EMC)is required for the co-translational insertion of newly synthesized multi-transmembrane proteins.Compromised EMC function in different cell types has been implicated in multiple diseases.Using inducible genetic mouse models,we revealed defects in retinal vascularization upon endothelial cell(EC)specific deletion of Emc1,the largest subunit of EMC.Loss of Emc1 in ECs led to reduced vascular progression and vascular density,diminished tip cell sprouts,and vascular leakage.We then performed an unbiased transcriptomic analysis on human retinal microvascular endothelial cells(HRECs)and revealed a pivotal role of EMC1 in theβ-catenin signaling pathway.Further in-vitro and in-vivo experiments proved that loss of EMC1 led to compromisedβ-catenin signaling activity through reduced expression of Wnt receptor FZD4,which could be restored by lithium chloride(LiCl)treatment.Driven by these findings,we screened genomic DNA samples from familial exudative vitreoretinopathy(FEVR)patients and identified one heterozygous variant in EMC1 that co-segregated with FEVR phenotype in the family.In-vitro expression experiments revealed that this variant allele failed to facilitate the expression of FZD4 on the plasma membrane and activate theβ-catenin signaling pathway,which might be a main cause of FEVR.In conclusion,our findings reveal that variants in EMC1 gene cause compromisedβ-catenin signaling activity,which may be associated with the pathogenesis of FEVR. | Shujin Li Mu Yang Rulian Zhao Li Peng Wenjing Liu Xiaoyan Jiang Yunqi He Erkuan Dai Lin Zhang Yeming Yang Yi Shi Peiquan Zhao Zhenglin Yang Xianjun Zhu | 2023 | Genes & Diseases2023，10，6： | 1 |
| 3 | Variants in the Wnt co-receptor LRP6 are associated with familial exudative vitreoretinopathy显示文摘Familial exudative vitreoretinopathy(FEVR),an inherited eye disease,is characterized by abnormal retinal vascular development,such as neovascularization,vitreous hemorrhage,exudation,and retinal detachment(Criswick and Schepens,1969;Robitaille et al.,2002).FEVR is inherited as autosomal dominant,autosomal recessive,and X-linked patterns(de Crecchio et al.,1998). | Shujin Li Mu Yang Yunqi He Xiaoyan Jiang Rulian Zhao Wenjing Liu Lulin Huang Yi Shi Xiao Li Kuanxiang Sun Yeming Yang Periasamy Sundaresan Peiquan Zhao Zhenglin Yang Xianjun Zhu | 2022 | Journal of Genetics and Genomics2022，49，6： | 1 |
| 4 | Loss of Wtap results in cerebellar ataxia and degeneration of Purkinje cells显示文摘N^(6)-methyladenosine(m^(6)A)modification,which is achieved by the METTL3/METTL14/WTAP methyltransferase complex,is the most abundant internal mRNA modification.Although recent evidence indicates that m^(6)A can regulate neurodevelopment as well as synaptic function,the roles of m^(6)A modification in the cerebellum and related synaptic connections are not well established.Here,we report that Purkinje cell(PC)-specific WTAP knockout mice display early-onset ataxia concomitant with cerebellar atrophy due to extensive PC degeneration and apoptotic cell death.Loss of Wtap also causes the aberrant degradation of multiple PC synapses.WTAP depletion leads to decreased expression levels of METTL3/14 and reduced m^(6)A methylation in PCs.Moreover,the expression of GFAP and NF-L in the degenerating cerebellum is increased,suggesting severe neuronal injuries.In conclusion,this study demonstrates the critical role of WTAP-mediated m^(6)A modification in cerebellar PCs,thus providing unique insights related to neurodegenerative disorders. | Yeming Yang Guo Huang Xiaoyan Jiang Xiao Li Kuanxiang Sun Yi Shi Zhenglin Yang Xianjun Zhu | 2022 | Journal of Genetics and Genomics2022，49，9： | 1 |
| 5 | The m^(6)A reader YTHDC2 maintains visual function and retinal photoreceptor survival through modulating translation of PPEF2 and PDE6B显示文摘Inherited retinal dystrophies (IRDs) are major causes of visual impairment and irreversible blindness worldwide, while the precise molecular and genetic mechanisms are still elusive. N6-methyladenosine (m^(6)A) modification is the most prevalent internal modification in eukaryotic mRNA. YTH domain containing 2 (YTHDC2), an m^(6)A reader protein, has recently been identified as a key player in germline development and human cancer. However, its contribution to retinal function remains unknown. Here, we explore the role of YTHDC2 in the visual function of retinal rod photoreceptors by generating rod-specific Ythdc2 knockout mice. Results show that Ythdc2 deficiency in rods causes diminished scotopic ERG responses and progressive retinal degeneration. Multi-omics analysis further identifies Ppef2 and Pde6b as the potential targets of YTHDC2 in the retina. Specifically, via its YTH domain, YTHDC2 recognizes and binds m^(6)A-modified Ppef2 mRNA at the coding sequence and Pde6b mRNA at the 5′-UTR, resulting in enhanced translation efficiency without affecting mRNA levels. Compromised translation efficiency of Ppef2 and Pde6b after YTHDC2 depletion ultimately leads to decreased protein levels in the retina, impaired retinal function, and progressive rod death. Collectively, our finding highlights the importance of YTHDC2 in visual function and photoreceptor survival, which provides an unreported elucidation of IRD pathogenesis via epitranscriptomics. | Yeming Yang Xiaoyan Jiang Junyao Chen Lu Liu Guo Liu Kuanxiang Sun Wenjing Liu Xianjun Zhu Qiuyue Guan | 2024 | Journal of Genetics and Genomics2024，51，2： | 0 |
| 6 | Experimental study on the correlation between hill direct seeding rate and field seedling rate of typical rice varieties in cold areas显示文摘In order to examine the relationship between the seeding rate of a hill-direct-seeding machine and seedling rate in field,six rice varieties typical for cold areas were selected to conduct the indoor germination tests and field seedling experiment using a hill-direct-seeding machine.Indoor germination and field seedling tests were performed to study the correlation among the mechanical precision seeding process,machine seeding rate and field seedling rate.The results showed that the indoor germination rates were higher than 90%.The series of spoons was adaptable to the hill-direct-seeding process.The number of seeds was 28-30,18-20 and 8-10 grains/hill.The field seedling rates were higher than 85%.The rice variety of Suijing-18 achieved the best field seedling rate,and the highest seeding rate was 18-20 grains/hill,while the average field seedling rate was 94.25%.On this basis,the action law of the hill-direct-seeding machine and the main factors influencing the field seedling rate were analyzed.Mechanical vibration resulted in a higher average seeding rate of the middle seed metering device of the hill-direct-seeding machine than that of both ends.The force between the seed spoons and rice seeds mechanically damaged the seed buds,resulting in the actual field seedling rate being lower than the ideal indoor germination rate.The experimental results can provide a reference for the selection of typical direct seeding rice varieties,the improvement of key components of hill-direct-seeding machines,and the promotion of agronomic models. | Han Tang Yeming Jiang Changsu Xu Wenqi Zhou Qi Wang Yijia Wang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，5： | 0 |
| 7 | The endoplasmic reticulum membrane protein complex subunit Emc6 is essential for rhodopsin localization and photoreceptor cell survival显示文摘The endoplasmic reticulum(ER)membrane protein complex(EMC)is responsible for monitoring the biogenesis and synthetic quality of membrane proteins with tail-anchored or multiple transmembrane domains.The EMC subunit EMC6 is one of the core members of EMC and forms an enclosed hydrophilic vestibule in cooperation with EMC3.Despite studies demonstrating that deletion of EMC3 led to rhodopsin mislocalization in rod photoreceptors of mice,the precise mechanism leading to the failure of rhodopsin trafficking remains unclear.Here,we generated the first rod photoreceptor-specific knockout of Emc6(RKO)and cone photoreceptor-specific knockout of Emc6(CKO)mouse models.Deficiency of Emc6 in rod photoreceptors led to progressive shortening of outer segments(OS),impaired visual function,mislocalization and reduced expression of rhodopsin,and increased gliosis in rod photoreceptors.In addition,CKO mice displayed the progressive death of cone photoreceptors and abnormal localization of cone opsin protein.Subsequently,proteomics analysis of the RKO mouse retina illustrated that several cilium-related proteins,particularly anoctamin-2(ANO2)and transmembrane protein 67(TMEM67),were significantly down-regulated prior to OS degeneration.Detrimental rod photoreceptor cilia and mislocalized membrane disc proteins were evident in RKO mice.Our data revealed that in addition to monitoring the synthesis of rhodopsin-dominated membrane disc proteins,EMC6 also impacted rod photoreceptors'ciliogenesis by regulating the synthesis of membrane proteins associated with cilia,contributing to the mislocalization of membrane disc proteins. | Kuanxiang Sun Lu Liu Xiaoyan Jiang Heting Wang Lin Wang Yeming Yang Wenjing Liu Lin Zhang Xiaohui Zhao Xianjun Zhu | 2024 | Genes & Diseases2024，11，2： | 0 |
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