1篇
您的检索式：作者名="Yinggui Xie"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Spatiotemporal expression of inducible nitric oxide synthase and cyclooxygenase 2 in the spinal cord during early stage sciatic nerve crush injury显示文摘BACKGROUND:Previous studies have shown that inducible nitric oxide synthase(iNOS) and cyclooxygenase 2(COX-2) participate in inflammatory immune responses and neuropathic pain following peripheral nerve injury.However,few reports have addressed time-dependent expression of iNOS and COX-2 following peripheral nerve injury.OBJECTIVE:To investigate spatiotemporal expression of iNOS and COX-2 during early stage sciatic nerve crush injury.DESIGN,TIME AND SETTING:The randomized,controlled,animal experiment was performed at the Laboratory of Applied Anatomy,Department of Human Anatomy and Neurobiology,Central South University,China from September 2006 to September 2007.MATERIALS:Mouse anti-rat iNOS monoclonal antibody and goat anti-rat COX-2 monoclonal antibody(Transduction Laboratory,USA),as well as biotinylated rabbit anti-mouse IgG and biotinylated rabbit anti-goat IgG(Santa Cruz Biotechnology,USA) were used in the present study.METHODS:A total of 48 healthy,adult,Sprague Dawley rats were randomly assigned to three groups.In the model group(n = 32),crush injury to the right sciatic nerve was established using an artery clamp.The model group was further assigned to four subgroups according to survival time(6,12,24,and 72 hours),respectively(n = 8).Sham surgery(n = 8) and normal control(n = 8) groups were also established.MAIN OUTCOME MEASURES:iNOS and COX-2 expression was detected in the L4-6 spinal cord with immunohistochemistry.Gray values of iNOS-and COX-2-postive cells in the anterior horn and posterior horn of spinal cord,as well as quantification of iNOS-and COX-2-positive cells in the anterior horn of spinal cord,were measured.RESULTS:iNOS and COX-2 expression gradually increased in the anterior horn and posterior horn of the spinal cord on the damaged side over time from 6 hours following sciatic nerve injury(P < 0.05) and peaked at 72 hours.Simultaneously,the number of iNOS-and COX-2-positive cells similarly increased in the anterior horn of spinal cord on the damaged side(P < 0.05).CONCLUSION:iNOS and COX-2 expression increased in the spinal cord during early stage sciatic nerve crush,which suggested that iNOS and COX-2 participate in occurrence and development of inflammatory immune responses following peripheral nerve injury. | Qiben Wang Linfeng Zheng Yinggui Xie Qinghong Huang He Huang Zhicheng Zeng Song Wu | 2010 | Neural Regeneration Research2010，5，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员