| 1 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Effects of 'Three Controls' Nutrient Management on Population Quality and Yield Components of Rice显示文摘[Objectives]This study was conducted to explore the control mechanism of agricultural non-point source pollution,and investigate the feasibility of promoting rice'three controls'nutrient management in Enping City.[Methods]With high-quality conventional rice as a material,such three treatments as three controls fertilization A(ZHY)and B(ZHY)and farmers conventional fertilization method FFP(ZXL)were set up,and the whole process of the late-season plot experiment was recorded.The agronomic characteristics of rice population quality and yield components during rice growth and development under the'three controls'fertilization technology were analyzed.[Results]Compared with the conventional fertilization method,the three controls A(ZHY)fertilization method improved rice yield by 27.13%,seed setting rate by 2.11%and 1 000-grain weight by 3.30%when reducing N,P and K by 27.13%,10.89%and 27.31%,respectively.In the case of three controls B(ZHY)omitting the last fertilization in the three controls fertilization method(4∶2∶3∶1),which saved the formula fertilizer by 11.25%,no difference was caused in yield,but the seed setting rate and 1 000-grain weight were still improved by 3.47%and 2.79%,respectively.Compared with the conventional fertilization method,the top first,second and third basal nodes of the three controls A(ZHY)fertilization method were shortened by 18.82%,17.06%and 20.52%,respectively,which plays an important role in combating typhoon and resisting lodging.[Conclusions]Compared with the conventional fertilization method,rice'three controls'nutrient management can improve yield and lodging resistance of rice,reduce fertilizer loss and agricultural non-point source pollution,and protect ecological environment. | Yongcong WU QianKai TAN Xiaoting YIN Qiwen LIANG Tingting LI Jinwei WANG Yuecun WU Tingting ZHOU | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
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