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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | The pharmacokinetics of the prostaglandin E1 analogue misoprostol in plasma and colostrum after postpartum oral administration显示文摘 | Hany Abdel-Aleem José Villar A Metin Gülmezoglu Sayed A Mostafa Alaa A Youssef Mahmoud Shokry Bernhard Watzer | 2002 | European Journal of Obstetrics and Gynecology2002，，1： | 2 |
| 5 | Early detection of hepatocellular carcinoma co-occurring with hepatitis C virus infection: A mathematical model显示文摘AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma(HCC) with a panel of serum proteins in combination with α-fetoprotein(AFP).METHODS: Serum levels of interleukin(IL)-8, soluble intercellular adhesion molecule-1(s ICAM-1), soluble tumor necrosis factor receptor Ⅱ(s TNF-RⅡ), proteasome, and β-catenin were measured in 479 subjects categorized into four groups:(1) HCC concurrent with hepatitis C virus(HCV) infection(n = 192);(2) HCV related liver cirrhosis(LC)(n = 96);(3) Chronic hepatitis C(CHC)(n = 96); and(4) Healthy controls(n = 95). The R package and different modules for binary and multi-class classifiers based on generalized linear models were used to model the data. Predictive power was used to evaluate the performance of the model. Receiver operating characteristic curve analysis over pairs of groups was used to identify the best cutoffs differentiating the different groups. RESULTS: We revealed mathematical models, based on a binary classifier, made up of a unique panel of serum proteins that improved the individual performance of AFP in discriminating HCC patients from patients with chronic liver disease either with or without cirrhosis. We discriminated the HCC group from the cirrhotic liver group using a mathematical model(-11.3 + 7.38 × Prot + 0.00108 × s ICAM + 0.2574 ×β-catenin + 0.01597 × AFP) with a cutoff of 0.6552, which achieved 98.8% specificity and 89.1% sensitivity. For the discrimination of the HCC group from the CHC group, we used a mathematical model [-10.40 + 1.416 × proteasome + 0.002024 × IL + 0.004096 × s ICAM-1 +(4.251 × 10-4) × s TNF + 0.02567 ×β-catenin + 0.02442 × AFP] with a cutoff 0.744 and achieved 96.8% specificity and 89.7% sensitivity. Additionally, we derived an algorithm, based on a binary classifier, for resolving the multi-class classification problem by using three successive mathematical model predictions of liver disease status. CONCLUSION: Our proposed mathematical model may be a useful method for the early detection of different statuses of liver disease co-occurring with HCV infection. | Abdel-Rahman Nabawy Zekri Amira Salah El-Din Youssef Yasser Mabrouk Bakr Reham Mohamed Gabr Ola Sayed Ahmed Mostafa Hamed Elberry Ahmed Mahmoud Mayla Mohamed Abouelhoda Abeer A Bahnassy | 2016 | World Journal of Gastroenterology2016，22，16： | 2 |
| 6 | The necessity of post- maneuver postural restriction in treating benign paroxysmal positional vertigo:a meta-analytic study显示文摘 | Mostafa BE Youssef TA Hamad AS | 2013 | EurArch of Oto-rhino- laryngology2013，270，3： | 1 |
| 7 | The necessity of post-maneuver postural restriction in treating benign paroxysmal positional vertigo:a metaanalytic study显示文摘 | MOSTAFA B E YOUSSEF T A HAMAD A S | 2013 | Eur Arch Otorhinolaryngol2013，270，： | 1 |
| 8 | Effects of isolated Lactobacillus acidophilus as a probiotic on chicken vaccinated and infected with Salmonella typhimurium 显示文摘 | Youssef G A Ezzeldeen N A Mostafa A M etal | 2011 | Global Veterinaria2011，7，5： | 1 |
| 9 | The keyhole retrosig-moid approach to the cerebello-pontine angle:indications,technical modifications,and results显示文摘 | Mostafa BE Sharnoubi ME Youssef AM | 2008 | Skull Base2008，18，6： | 1 |
| 10 | Glassy nature of water in an ultracomfining disorderedmaterial:the case of calcium-silicate-hydrate显示文摘 | Youssef Mostafa Pellenq Roland J M Yildiz Bilge | 2011 | J AmChem Soc2011，133，： | 1 |
| 11 | The necessity of post-maneu- ver postural restriction in treating benign paroxysmal positional ver- tigo: a meta-analytic study 显示文摘 | Mostafa BE Youssef TA Hamad AS | 2013 | Eur Arch Otorhinolaryngol2013，270，： | 1 |
| 12 | Behavior of I-beam bolted extended end-plate moment connections显示文摘 | Abdelrahim Khalil Dessouki Ahmed Hassan Youssef Mohamed Mostafa Ibrahim | 2013 | Ain Shams Engineering Journal2013，，： | 1 |
| 13 | The necessity of post- maneuver postural restriction in treating benign paroxysmal positional vertigo:a meta-analytic study 显示文摘 | Mostafa BE Youssef TA Hamad AS | 2013 | Eur Arch O- torhinolaryngol2013，270，3： | 1 |
| 14 | The keyhole retrosigmoid approach to the cerebello-pontine angle:indications,technical modifications,and results显示文摘 | Mostafa BE Sharnoubi ME Youssef AM | | 0，，06： | 1 |
| 15 | The keyhole retrosig- moid approach to the cerebellopontine angle:indications, technical modifications, and results显示文摘 | Mostafa BE Sharnoubi M Youssef AM | 2008 | Skull Base2008，18，6： | 1 |
| 16 | The keyhole retrosigmoid approach to the cerebe 11 opontine angle: indications, technical modifications, and results 显示文摘 | MOSTAFA B E SHARNOUBI M YOUSSEF A M | 2008 | Skull Base2008，18，6： | 1 |
| 17 | The necessity of post- maneuver postural restriction in treating benign paroxysmalpositional vertigo :a meta-analytic study 显示文摘 | Mostafa BE Youssef TA Hamad AS | 2013 | Eur Arch O-torhinolaryngol2013，270，3： | 1 |
| 18 | The keyhole ret- rosigmoid approach to the cerebellopontine angle: indications, technical modifications, and results 显示文摘 | Mostafa BE EL Sharnoubi M Youssef AM | 2008 | Skull Base2008，18，6： | 1 |
| 19 | Vaginal douching by women with vulvovaginitis and relation to reproductive health hazards显示文摘 | Omar M Shaaban Alaa Eldin A Youssef Mostafa M Khodry | 2013 | BMC Womens Health2013，，： | 1 |
| 20 | The keyhole retrosigmoid approach to the eerebello-pontine angle: indications, technical modifications, and results 显示文摘 | Mostafa BE E1 Shamoubi M Youssef AM | 2008 | Skull Base2008，18，6： | 1 |
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