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| 1 | Structure change of 430 stainless steel in the heating process显示文摘The microstructure analysis was employed for the ferritic stainless steel(SUS430)with the carbon content from 0.029wt% to 0.100wt% under the simulated heating process condition.The higher carbon sample(430H)contains the duplex phase micro- structure at the temperature of 1150℃;on the other hand,the lower carbon content sample(430L)does not touch two phase area even at the temperature of 1450℃and has the single phase ferritic microstructure.The carbon content need be well controlled for the 430 ferritic stainless steel since it can significantly affect the heating process curve,and the heating process may not be done in the two phase area due to the uncontrolled carbon content.With the low carbon content and the proper soaking time,the grain size is not sensitive to the heating process temperature and the soaking time.In the present heat treatment experiments,the soaking time is about 10 min,and the processing parameters can be chosen according to the requirement of the gross energy,the efficiency and the continual forming. | Xinzhong Liu Jingtao Han Wanhua Yu Shifeng Dai | 2008 | Journal of University of Science and Technology Beijing2008，15，1： | 6 |
| 2 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 3 | Energy Analysis of a CPG-controlled Miniature Robotic Fish显示文摘 | Junzhi Yu Shifeng Chen Zhengxing Wu Xingyu Chen Ming Wang | 2018 | Journal of Bionic Engineering2018，15，2： | 5 |
| 4 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 5 | Overvoltage and Insulation Coordination of Jinping-Sunan ±800 kV UHVDC Porject Converter Stations显示文摘 | YU Shifeng NIE Dingzhen MA Weimin TONG Yuliang | 2012 | 高电压技术2012，38，12： | 2 |
| 6 | PhysicochemicalProperties of Starch and Flour from Different Rice Cultivars 显示文摘 | Shifeng Yu’Ying Ma Lucile Menager | 2012 | Food Bioprocess Technol2012，，5： | 1 |
| 7 | The effect of Epimedium grandiflorum mort on prevention and treatment of rat mandible and femur bone loss显示文摘 | Li Shenglin Zhang Kuihua Yu Shifeng | 2007 | Chin J Dent Res2007，10，： | 1 |
| 8 | Impact of amylose content on starch retrogradation and texture of cooked milled rice during storage显示文摘 | Yu Shifeng Ma Ying Sun Dawen | | 0，，02： | 1 |
| 9 | Clear cell odontogenic carcinoma : A clinicopathological and immunocytochemical study of five cases显示文摘 | Li Tiejun Yu Shifeng Gao Yan | 2001 | Arch Pathol Lab Med2001，125，12： | 1 |
| 10 | Impact of amylose content on starch retrogradation and texture of cooked milled rice dur- ing storage显示文摘 | Yu Shifeng Ma Ying Sun Dawen | 2009 | Journal of Cereal Science2009，50，2： | 1 |
| 11 | Experimental study on the effect of Puerarin on bone metabolism in viro 显示文摘 | Li Binbin Yu Shifeng | 2003 | Journal of Peking University2003，35，1： | 1 |
| 12 | Impact of amyIose con- tent on starch retrogradation and texture of cooked milled rice during storage 显示文摘 | YU Shifeng MA Ying SUN Dawen | 2009 | Journal of Cereal Science2009，50，2： | 1 |
| 13 | Supercritical fluid extraction and micronization of ginkgo flavonoids from ginkgo biloba leaves显示文摘 | Miao Shifeng Yu Jinpeng Du Zhe | 2010 | Industrial & Engineering Chemistry Research2010，9，11： | 1 |
| 14 | Impact of amylose content on starch retrogradation and texture of cooked milled rice during storage显示文摘 | YU Shifeng MA Ying SUN Dawen | 2009 | Journal of Cereal Science2009，50，2： | 1 |
| 15 | Impact of cooling rates on the staling behavior of cooked rice during storage显示文摘 | Yu Shifeng Ma Ying Liu Tianyi | 2010 | Journal of Food Engineering2010，96，： | 1 |
| 16 | 显示文摘 | Yu Zhenyang Yin Jingbo Yan Shifeng | 2007 | Polymer2007，48，： | 1 |
| 17 | Biode- gradable Poly ( L-lactide)/Poly ( 3-caprolactone)-modified Montmorillonite Nanocomposites Preparation' and Char- acterization显示文摘 | Yu Zhenyang Yin Jingbo Yan Shifeng | 2007 | Polymer2007，48，： | 1 |
| 18 | Biodegradable poly(L-lactide) / poly ( ε -caprolactone)-modified montmorillonite nanocomposites:Preparation and characterization显示文摘 | Yu Zhengyang Yin Jingbo Yan Shifeng | 2007 | Polymer2007，48，21： | 1 |
| 19 | Physicochemical properties of starch and flour from different rice cultivars显示文摘 | Yu Shifeng Ma Ying Menager L | 2012 | Food Bioprocess Technology2012，5，： | 1 |
| 20 | Impact of cooling rates on the staling behavior of cooked rice during storage显示文摘 | Shifeng Yu Ying Ma Tianyi Liu | 2010 | Journal of Food Engineering2010，，96： | 1 |
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