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| 1 | Single-cell transcriptomic profiling reveals the tumor heterogeneity of small-cell lung cancer显示文摘Small-cell lung cancer(SCLC)is the most aggressive and lethal subtype of lung cancer,for which,better understandings of its biology are urgently needed.Single-cell sequencing technologies provide an opportunity to profile individual cells within the tumor microenvironment(TME)and investigate their roles in tumorigenic processes.Here,we performed high-precision single-cell transcriptomic analysis of~5000 individual cells from primary tumors(PTs)and matched normal adjacent tissues(NATs)from 11 SCLC patients,including one patient with both PT and relapsed tumor(RT).The comparison revealed an immunosuppressive landscape of human SCLC.Malignant cells in SCLC tumors exhibited diverse states mainly related to the cell cycle,immune,and hypoxic properties.Our data also revealed the intratumor heterogeneity(ITH)of key transcription factors(TFs)in SCLC and related gene expression patterns and functions.The non-neuroendocrine(non-NE)tumors were correlated with increased inflammatory gene signatures and immune cell infiltrates in SCLC,which contributed to better responses to immune checkpoint inhibitors.These findings indicate a significant heterogeneity of human SCLC,and intensive crosstalk between cancer cells and the TME at single-cell resolution,and thus,set the stage for a better understanding of the biology of SCLC as well as for developing new therapeutics for SCLC. | Yanhua Tian Qingqing Li Zhenlin Yang Shu Zhang Jiachen Xu Zhijie Wang Hua Bai Jianchun Duan Bo Zheng Wen Li Yueli Cui Xin Wang Rui Wan Kailun Fei Jia Zhong Shugeng Gao Jie He Carl MGay Jianjun Zhang Jie Wang Fuchou Tang | 2022 | Signal Transduction and Targeted Therapy2022，7，11： | 1 |
| 2 | Insights into the mechanism of shortened developmental duration and accelerated weight gain associated with Wolbachia infection in Hylyphantes graminicola显示文摘Wolbachia infection is known to affect host reproduction and development.To date,however,the underlying mechanism related to the effects of Wolbachia on host development has not yet been reported.Here,we compared the developmental duration and body weight in different instars of Wolbachia-positive(W+)and Wolbachia-negative(W−)spiders(Hylyphantes graminicola)and detected the relative expression levels of 6 insulin-related genes and 3 ecdysone-related genes using reverse transcription qPCR.Results showed that the developmental duration was significantly shortened in W+spiders compared with W−spiders.Furthermore,W+spiders were significantly heavier thanW−spiders at the 3rd and 4th instars,although no significant differences in body weight were observed after maturity.We also found that the expression levels of insulin-like growth factor-2 mRNA-binding protein-1,insulin-degrading enzyme,and ecdysone-inducible protein-1 genes were significantly down-regulated in W+spiders compared with W−spiders,whereas the expression levels of insulin-like growth factor-binding protein-1,insulin-like peptide receptor,insulin receptor substrate 2-B,insulin-like,ecdysone-induced protein-2,and ecdysone receptor genes were significantly up-regulated inW+spiders.Our results suggest thatWolbachia may influence host development by affecting insulin and ecdysone signaling pathways. | Chunfen LI Meng HE Yinjie CUI Yu PENG Jie LIU Yueli YUN | 2022 | Integrative Zoology2022，17，3： | 0 |
| 3 | 人类心脏发育的单细胞转录组研究显示文摘文章简介本研究利用高精度的单细胞转录组测序等技术,全面、系统地解析了人类心脏发育过程中各种主要细胞类型的重要生物学特点,揭示了心脏发育过程中关键信号通路的时空特异性激活特征以及心肌细胞与非心肌细胞之间的复杂信号互作机制。同时通过与已发表的小鼠心脏发育单细胞转录组数据的系统比较。 | Yueli Cui Yuxuan Zheng Xixi Liu Liying Yan Xiaoying Fan Jun Yong Yuqiong Hu Ji Dong Qingqing Li Xinglong Wu Shuai Gao Jingyun Li Lu Wen 乔杰 汤富酬 | 2020 | 科学新闻2020，，2： | 0 |
| 4 | Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration显示文摘Mammals exhibit limited heart regeneration ability,which can lead to heart failure after myocardial infarction.In contrast,zebrafish exhibit remarkable cardiac regeneration capacity.Several cell types and signaling pathways have been reported to participate in this process.However,a comprehensive analysis of how different cells and signals interact and coordinate to regulate cardiac regeneration is unavailable.We collected major cardiac cell types from zebrafish and performed high-precision single-cell transcriptome analyses during both development and post-injury regeneration.We revealed the cellular heterogeneity as well as the molecular progress of cardiomyocytes during these processes,and identified a subtype of atrial cardiomyocyte exhibiting a stem-like state which may transdifferentiate into ventricular cardiomyocytes during regeneration.Furthermore,we identified a regeneration-induced cell(RIC)population in the epicardium-derived cells(EPDC),and demonstrated Angiopoietin 4(Angpt4)as a specific regulator of heart regeneration.angpt4 expression is specifically and transiently activated in RIC,which initiates a signaling cascade from EPDC to endocardium through the Tie2-MAPK pathway,and further induces activation of cathepsin K in cardiomyocytes through RA signaling.Loss of angpt4 leads to defects in scar tissue resolution and cardiomyocyte proliferation,while overexpression of angpt4 accelerates regeneration.Furthermore,we found that ANGPT4 could enhance proliferation of neonatal rat cardiomyocytes,and promote cardiac repair in mice after myocardial infarction,indicating that the function of Angpt4 is conserved in mammals.Our study provides a mechanistic understanding of heart regeneration at single-cell precision,identifies Angpt4 as a key regulator of cardiomyocyte proliferation and regeneration,and offers a novel therapeutic target for improved recovery after human heart injuries. | Zekai Wu Yuan Shi Yueli Cui Xin Xing Liya Zhang Da Liu Yutian Zhang Ji Dong Li Jin Meijun Pang Rui-Ping Xiao Zuoyan Zhu Jing-Wei Xiong Xiangjun Tong Yan Zhang Shiqiang Wang Fuchou Tang Bo Zhang | 2023 | Protein & Cell2023，14，5： | 0 |
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