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| 1 | Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharideinduced inflammatory responses in broilers显示文摘Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicate with the host have not been clearly understood.Bacterial extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely studied.Here,we investigated the regulatory actions of EVs produced by L.reuteri BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro experiments.Results:L.reuteri BBC3 produced nano-scale membrane vesicles with the size range of 60-250 nm.Biochemical and proteomic analyses showed that L.reuteri BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor Tu.In vivo broiler experiments showed that administration of LrEVs exerted similar effects as L.reuteri BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal injury.LrEVs suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the jejunum.LrEVs could be internalized by chicken macrophages.In vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken macrophages.Additionally,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macrophages.Finally,we revealed that the reduced content of both vesicular proteins and nucleic acids attenuated the suppression of LrEVs on LPS-induced inflammatory responses in ex vivo experiments,suggesting that they are essential for the LrEVs-mediated immunoregulation.Conclusions:We revealed that LrEVs participated in maintaining intestinal immune homeostasis against LPSinduced inflammatory responses in a chicken model.Our findings provide mechanistic insight into how commensal and probiotic Lactobacillus species modulate the host’s immune system in pathogens-induced inflammation. | Rujiu Hu Hua Lin Mimi Wang Yuezhen Zhao Haojing Liu Yuna Min Xiaojun Yang Yupeng Gao Mingming Yang | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 5 |
| 2 | TGF-β-miR-34a-CCL22 Signaling-Induced Treg Cell Recruitment Promotes Venous Metastases of HBV-Positive Hepatocellular Carcinoma显示文摘 | Pengyuan Yang Qi-Jing Li Yuxiong Feng Yun Zhang Geoffrey J. Markowitz Shanglei Ning Yuezhen Deng Jiangsha Zhao Shan Jiang Yunfei Yuan Hong-Yang Wang Shu-Qun Cheng Dong Xie Xiao-Fan Wang | 2012 | Cancer Cell2012，，3： | 4 |
| 3 | FGFR/RACK1 interacts with MDM2, promotes P53 degradation, and inhibits cell senescence in lung squamous cell carcinoma显示文摘Objective:FGFR is considered an important driver gene of lung squamous cell carcinoma(LSCC).Thus,identification of the biological events downstream of FGFR is important for the treatment of this malignancy.Our previous study has shown that the FGFR/RACK1 complex interacts with PKM2 and consequently promotes glycolysis in LSCC cells.However,the biological functions of the FGFR/RACK1 complex remain poorly understood.Methods:Anchorage-independent assays and in vivo tumorigenesis assays were performed to evaluate cancer cell malignancy.Distant seeding assays were performed to evaluate cancer cell metastasis.β-gal staining was used to examine cell senescence,and immunoprecipitation assays were performed to examine the interactions among FGFR,RACK1,and MDM2.Results:FGFR/RACK1 was found to regulate the senescence of LSCC cells.Treatment with PD166866,an inhibitor of FGFR,or knockdown of RACK1 induced senescence in LSCC cells(P<0.01).A molecular mechanistic study showed that FGFR/RACK1/MDM2 form a complex that promotes the degradation of p53 and thus inhibits cell senescence.PD166866 and RG7112,an MDM2/p53 inhibitor,cooperatively inhibited the colony formation and distal seeding of LSCC cells(P<0.01),and upregulated the expression of p53 and p21.Conclusions:Together,our findings revealed the regulatory roles and mechanisms of FGFR/RACK1 in cell senescence.This understanding should be important in the treatment of LSCC. | Tao Chen Fei Wang Shupei Wei Yingjie Nie Xiaotao Zheng Yu Deng Xubin Zhu Yuezhen Deng Nanshan Zhong Chengzhi Zhou | 2021 | Cancer Biology & Medicine2021，18，3： | 3 |
| 4 | 胸腔置管白介素-2联合顺铂治疗恶性胸腔积液的临床观察(英文)显示文摘Objective: The aim of our study was to explore the short-term effects and complication of interleukin-2 (IL-2) combining with Cisplatin in the treatment of malignant hydrothorax.Methods: Sixty-two cases patients with malignant hydrothorax were randomly divided into two groups.Observation group was 31 examples,thoracic cavity injection IL-2 and Cisplatin;31 cases in control group using Cisplatin alone intra-thoracic injection.The regime of every week for 1–4 weeks was used to observe short term effects and complications.Results: The total response rate in observe group was higher than that in control group (90.3% vs.68.1%),which had statistically significant difference (P < 0.05).The complications included gastrointestinal tract reaction,bone marrow inhibition,chest pain and fever.The incidence rates of chest pain and fever in observe group was slightly higher than that in control group,but there was no statistically significant difference (P > 0.05).Conclusion: The IL-2 combining with Cisplatin intra-thoracic injection for malignant hydrothorax has the features of good therapeutic effects and slight poisonous side effects,which is worth to be used in clinic. | Cheng Xu Zaijun Zhang Yuezhen Hu Yeyuan Wang Mingquan Li | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，1： | 3 |
| 5 | Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice显示文摘Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice.The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform.Eighty operational taxonomic unit were identified to be shared by all samples,and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves.The diversity of gut flora was slightly improved in diabetic mice after GFP treatment.The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP.The abundance of Robinsoniella,Flavonifractor,Anaerotruncus,and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis.Furthermore,Alloprevotella and Burkholderia had strong relevancy with other gut flora.Based on the findings,GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes. | Xiaoxiang Gao Dan Liu Luying Gao Yuezhen Ouyang Yuxi Wen Chao Ai Yuqing Chen Chao Zhao | 2022 | Food Science and Human Wellness2022，11，1： | 2 |
| 6 | Analyzing the dynamics of Dafeng coastal wetland based on remote sensing image显示文摘 | Yuezhen SHI Dongjun XIN | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 2 |
| 7 | Effect of Zibushenjing Fang on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency: a mechanistic study显示文摘OBJECTIVE: To investigate the effects of Zibushenjing Fang (formula for tonifying the kidney essence) on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency. METHODS: Fifty male Kunming mice were randomly divided into five groups: normal group, model group, Jinkuishenqi Wan group, Zibushenjing Fang high dose group, and Zibushenjing Fang low dose group, with 10 mice in each group. The model of kidney essence insufficiency syndrome was established in all the mice except the normal group by using a cat to threaten the mice and by swimming until exhaustion daily which lasted about 21 days. Mice in the model group were administered 20 mL/ kg -1 · d -1 of normal saline intragastrically. The Jinkuishenqi Wan group was given 2.7 g/kg-1 · d-1 ofa solution of Jinkuishenqi Wan. The Zibushenjing Fang high dose group was given 20 g/kg -1 · d -1 and the Zibushenjing Fang low dose group was given 10 g/kg -1 · d -1 of a solution of Zibushenjing Fang.The general condition of all the groups was observed, including the quantity of food and water intake, swimming time, length of femur, and weight of the femur and musculus quadriceps femoris. The total superoxide dismutase (T-SOD), glutathione peroxi- dase (GSH-Px), and methane dicarboxylic aldehyde (MDA) activities in the brain tissues were detected. RESULTS: Zibushenjing Fang could improve the manifestation of kidney essence insufficiency, increase the quantity of food and water intake, swimming time, femur length, and femur and musculus quadriceps femoris weight. It could also regulate the activities of T-SOD, GSH-Px, and MDA in brain tissue. CONCLUSION: Zibushenjing Fang may play an important role in treating kidney essence insufficiency syndrome by promoting body development and improving brain tissue antioxidation. | Yuezhen He Junxiao Yao Shilin Shen Xiaojun Su Yuping Wang Li Li Wenhui Wang Jiping Shi | 2013 | Journal of Traditional Chinese Medicine2013，33，3： | 2 |
| 8 | Effect of Chemical Crosslinking on Mechanical and Electrical Properties of Ultrahigh-molecular-weight Polyethy- lene-carbonfiber Blends Prepared by Gelation/Crystalliza- tion from Solutions 显示文摘 | Ru Chen Yuezhen Bin Yumiko Nakano Naoko Kurata | 2010 | Colloid Polym Sci2010，288，： | 1 |
| 9 | Studies on graft copolymerization of chitosan with acrylonitrile by the redox system显示文摘 | Lv Pengju Bin Yuezhen Li Yongqiang | 2009 | Polymer2009，50，24： | 1 |
| 10 | Small angle X-ray scattering from voids within fibers during the stabilization and carbonization stages显示文摘 | Miyoshi Kaburagi Yuezhen Bin Dan Zhu | 2003 | Carbon2003，41，： | 1 |
| 11 | Simultaneous determination of rifampicin and isoniazid by continuous-flow chemiluminescence with artificial neural network calibration显示文摘 | Baoxin Li Yuezhen He Jiagen Lv Zhujun Zhang | 2005 | Analytical and Bioanalytical Chemistry2005，，5： | 1 |
| 12 | Finite element analysis of a bus skeleton and structure im-provement 显示文摘 | JIANG Fachao FAN Yuezhen LIU Entuo | 2013 | Adv Intell Syst Comput2013，180，： | 1 |
| 13 | Relations of weight-average molecular weights and the thermal properties of N-cvclohexyl maleimide-methyl methacrylate显示文摘 | Dong Shaosheng Wang Qingmin Wei Yuezhen | 1998 | J Appl Polym Sci1998，，70： | 1 |
| 14 | The study progress of Paclitaxel cardiac toxicity显示文摘 | YANG Xingyan XUE Yuezhen | 2009 | Herald of Medicine2009，28，8： | 1 |
| 15 | Positive temperature coefficient effect of LMWPE-UHMWPE blends filled with short carbon fibers显示文摘 | Xi Ying Ishikawa H Bin Yuezhen | 2004 | Carbon2004，42，： | 1 |
| 16 | Effect of chemical crosslinking on mechanical and electrical properties of ultrahigh-molecular-weight polyethylene-carbon fiber blends prepared by gelation/ crystallization from solution显示文摘 | Chen Ru Bin Yuezhen Nakano Y | 2010 | Colloid Polym Sci2010，288，： | 1 |
| 17 | The effect of vertical transports of heat and moisture by cumulus convection in typhoon显示文摘 | Yihui Ding Yuezhen Liu | 1987 | Advances in Atmospheric Sciences1987，，3： | 1 |
| 18 | Catalytic effect of nickel under carbonization of polyimide films 显示文摘 | YUEZHEN BIN KUMIKO OISHI AI KOGANEMMARU | 2005 | Carbon2005，43，8： | 1 |
| 19 | Electrical and mechanical properties of polyimide - carbon nanotubes composites fabricated by in situ polymerization 显示文摘 | Xiaowen Jiang Yuezhen Bin Masaru Matsuo | 2005 | Polymer2005，46，： | 1 |
| 20 | Morphology and mechanical and electrical properties of oriented PVA-VGCF and PVA- MWNT composites显示文摘 | Yuezhen Bin Mariko Mine Ai Koganemam | 2006 | Polymer2006，47，： | 1 |
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