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| 1 | Schisandra sphenanthera extract(Wuzhi Tablet)protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway in mice显示文摘Alcohol abuse leads to alcoholic liver disease and no effective therapy is currently available.Wuzhi Tablet(WZ), a preparation of extract from Schisandra sphenanthera that is a traditional hepatoprotective herb, exerted a significant protective effect against acetaminophen-induced liver injury in our recent studies, but whether WZ can alleviate alcohol-induced toxicity remains unclear. This study aimed to investigate the contribution of WZ to alcohol-induced liver injury by using chronic-binge and acute models of alcohol feeding. The activities of ALT and AST in serum were assessed as well as the level of GSH and the activity of SOD in the liver. The expression of CYP2E1 and proteins in the NRF2-ARE signaling pathway including NRF2, GCLC, GCLM, HO-1 were measured, and the effect of WZ on NRF2 transcriptional activity was determined. We found that both models resulted in liver steatosis accompanied by increased transaminase activities, but that liver injury was significantly attenuated by WZ. WZ administration also inhibited CYP2E1 expression induced by alcohol, and elevated the level of GSH and the activity of SOD in the liver. Moreover, the NRF2-ARE signaling pathway was activated by WZ andthe target genes were all upregulated. Furthermore, WZ significantly activated NRF2 transcriptional activity. Collectively, our study demonstrates that WZ protected against alcohol-induced liver injury by reducing oxidative stress and improving antioxidant defense, possibly by activating the NRF2-ARE pathway. | Xuezhen Zeng Xi Li Chenshu Xu Fulin Jiang Yufei Mo Xiaomei Fan Yaoting Li Yiming Jiang Dongshun Li Min Huang Huichang Bi | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 13 |
| 2 | A competitive RT-PCR-based approach to quantifying promoter activity of stress gene显示文摘Chloramphenicol acetyltransferase (CAT) as a stable reporter has been broadly adopted to detect promoter activity of many stress genes in eukaryotic cells. A simplified, convenient and relatively accurate assay to reveal CAT activities driven by heat shock gene promoter upon cell stress is reported here. A CAT mutant control plasmid pMCAT is constructed out of pBLCAT3, with a DNA segment within the CAT gene deleted. The control plasmid pMCAT along with the wild type CAT reporter gene driven by the regulatory sequence of hsp90β gene are co-transfected into Jurkat cells. Following total cellular RNA extracted and reverse transcribed, PCR is carried out to amplify two individual fragments with the same pair of synthesized primers, which bind to the identical sequences in both pMCAT and the CAT reporter plasmids. The two PCR fragments amplified from the reporter and control RNAs are identified by their size differences in agarose gel electrophoresis. The ratio of intensity of the two PCR bands in the same lane is taken as 'relative units of promoter efficiency' of a gene. The transcripts instead of CAT activity thus detected, may not only provide a closer look at the promoter activity of the stress gene but also normalize the efficiency of transfection simultaneously. With experimental conditions optimized, visible and reliable results can be obtained without using any sophisticated instruments. Since the results obtained with the RT-PCR approach are reproducible, much less troublesome and time-consuming, and comparable to our previous data, we thus take it as a conventional procedure for promoter activity assay of human hsp gene upon heat shock. Additionally, results from the assay have recently provided the first evidence that Rac, MEKK and JNK signals participate in the heat shock-induced expression of hsp90β gene. | MO Zhicheng, LI Xiaoyan, LI Hongfan, CHENG Xiaokuan, WU Ninghua and SHEN Yufei(National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China) | 2002 | Progress in Natural Science:Materials International2002，12，10： | 4 |
| 3 | Mass spectrometry-based proteomic approaches to study pathogenic bacteria-host interactions显示文摘位于主人病原体相互作用下面的分子的机制的说明为世界范围的传染疾病的控制和处理是重要的。在最后十年以内,集体 spectrometry (MS ) 基于 proteomics 成为了一条强大、有效的途径更好在蛋白质水平理解复杂、动态的主人病原体相互作用。此处,我们将与伤寒病原体的一个特别焦点向他们的哺乳动物的主人的细菌的感染在 proteomic 分析考察最近的进步。动态 proteomic 改变的大规模研究将从病原的细菌和宿主细胞的观点在感染期间被讨论。 | Yufei Yang Mo Hu Kaiwen Yu Xiangmei Zeng Xiaoyun Liu | 2015 | Protein & Cell2015，6，4： | 1 |
| 4 | Micro/nano-tribological behaviors of crown-type phosphate ionic liquid ultrathin films on self-assembled monolayer modified silicon显示文摘 | Jibin Pu Dong Jiang Yufei Mo Liping Wang Qunji Xue | 2011 | Surface & Coatings Technology2011，，20： | 1 |
| 5 | Mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes synthesized in deep eutectic solvent and their catalytic performances显示文摘To develop high-performance metal-organic frameworks(MOFs)for catalysis is of great importance.Here,we synthesized the mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)(BTC=benzene-1,3,5-tricarboxylate)nanocubes in a deep eutectic solvent of ZnCl_(2)/ethylene glycol solution.The route can proceed at room temperature and the reaction time needed is shortened to be 30 min,which is superior to the conventional solvothermal route that usually needs high temperature and long reaction time.The formation mechanism of the mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes in deep eutectic solvent(DES)was investigated by in situ synchrotron X-ray diffraction/small angle X-ray scattering/X-ray absorption fine structure conjunction technique.The mesoporous Cu_(3−x)Zn_(x)(BTC)_(2)nanocubes exhibit high catalytic activity and reusability for cyanosilylation reaction of benzaldehyde and aerobic oxidation reaction of benzylic alcohol. | Jingyang Hu Jianling Zhang Xiuniang Tan Xiuyan Cheng Zhuizhui Su Lixiong Qian Mingzhao Xu Yufei Sha Yanyue Wang Yisen Yang Yunpeng Liu Guang Mo Xueqing Xing Zhonghua Wu | 2023 | Nano Research2023，16，3： | 0 |
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