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| 1 | 影响内镜医师诊断萎缩性胃炎的因素分析显示文摘目的探讨各种因素对内镜医师诊断慢性萎缩性胃炎(CAG)准确性的影响。方法采用回顾性分析的方法,收集上海交通大学医学院附属仁济医院2009年1月至12月间行内镜检查的10765例慢性胃炎患者,分析内镜下充血渗出、糜烂、胃溃疡、胆汁反流、胃息肉病例及幽门螺杆菌(Hp)感染对CAG内镜及病理诊断的影响。结果病理检查CAG的诊断率为69.41%,内镜检查CAG的诊断率为54.27%,两者符合率为62.30%。Hp阳性者2575例(23.92%),Hp阳性者的CAG内镜和病理诊断符合率是Hp阴性者的90%(β=-0.1067,P〈0.05)。年龄和符合率成正相关。年龄每增加1岁,内镜和病理诊断符合率增加了0.01倍[OR—exp(0.00855)=1.01];每增加10岁符合率增加了0.09倍[OR—exp(0.0855)=1.091。胃黏膜充血渗出和符合率成负相关。有充血渗出者的CAG内镜和病理诊断符合率是无充血渗出者的40%(β=-0.9044,P〈0.01)。结论内镜下对CAG的判断有一定主观性,需结合病理分析方可确诊。患者年龄、Hp感染、内镜下充血渗出会影响CAG的内镜和病理诊断的符合率。 | 胡靥 Eugene Yuo Hao Chooi 陈晓宇 戈之铮 房静远 | 2012 | 中华消化杂志2012，32，4： | 8 |
| 2 | 饮食因素对慢性萎缩性胃炎转归的影响显示文摘慢性萎缩性胃炎(chronic atrophy gastritis,CAG)是最主要的胃癌癌前病变。其发生与幽门螺杆菌(Helicobacter Pylori,Hp)感染、遗传因素、环境因素和用药史等均有关。饮食因素对其转归的影响报道较少。本研究回顾性分析了3156例CAG患者饮食因素与其内镜和病理组织学转归的关系。 | Eugene Yuo Hao Chooi 房静远 | 2011 | 中华消化杂志2011，31，8： | 7 |
| 3 | Human pluripotent stem cells:Towards therapeutic development for the treatment of lifestyle diseases显示文摘There are two types of human pluripotent stem cells: Embryonic stem cells(ESCs) and induced pluripotent stem cells(iPSCs),both of which launched themselves on clinical trials after having taken measures to overcome problems: Blocking rejections by immunosuppressants regarding ESCs and minimizing the risk of tumorigenicity by depleting exogenous gene components regarding iP SCs.It is generally assumed that clinical applications of human pluripotent stem cells should be limited to those cases where there are no alternative measures for treatments because of the risk in transplanting those cells to living bodies.Regarding lifestyle diseases,we have already several therapeutic options,and thus,development of human pluripotent stem cell-based therapeutics tends to be avoided.Nevertheless,human pluripotent stem cells can contribute to the development of new therapeutics in this field.As we will show,there is a case where only a short-term presence of human pluripotent stem-derived cells can exert long-term therapeutic effects even after they are rejected.In those cases,immunologically rejections of ESC-or allogenic iP SC-derived cells may produce beneficial outcomes by nullifying the risk of tumorigenesis without deterioration of therapeutic effects.Another utility of human pluripotent stem cells is the provision of an innovative tool for drug discovery that are otherwise unavailable.For example,clinical specimens of human classical brown adipocytes(BAs),which has been attracting a great deal of attention as a new target of drug discovery for the treatment of metabolic disorders,are unobtainable from living individuals due to scarcity,fragility and ethical problems.However,BA can easily be produced from human pluripotent stem cells.In this review,we will contemplate potential contribution of human pluripotent stem cells to therapeutic development for lifestyle diseases. | Miwako Nishio Masako Nakahara Akira Yuo Kumiko Saeki | 2016 | World Journal of Stem Cells2016，8，2： | 2 |
| 4 | A cross-national comparative analysis of innovation policy in the integrated circuit industry显示文摘 | Joseph Z Shyu Yi-Chia Chiu Chao-Chen Yuo | 2001 | Technology in Society2001，，2： | 1 |
| 5 | Functional characterization of barley betaglucanless mutants demonstrates a unique role for Cs显示文摘 | Taketa S Yuo T Tonooka T | 2012 | Journal of Experimental Botany2012，63，1： | 1 |
| 6 | Bcl-2 down-regulation causes autophagy in a caspase-independent manner in human leukemic HL60 cells显示文摘 | Saeki K Yuo A Okuma E | 2000 | Cell Death Differ2000，7，12： | 1 |
| 7 | Radiation pneumonitis following combined modality therapy for lung cancer: analysis of prognostic factors显示文摘 | Roach M 3rd Gandara DR Yuo HS | 1995 | J Clin Oncol1995，13，10： | 1 |
| 8 | On the optimality of multiantenna broadcast scheduling using zero-forcing beamforming显示文摘 | Yuo T Goldsmith A | 2006 | IEEE Journal on Selected Areas in Communications2006，24，3： | 1 |
| 9 | Distinct involvement of cAMP response element dependent transcriptions in functional and morphological maturation during retinoid mediated human myeloid differentiation显示文摘 | Saeki K Yuo A | 2003 | J Leukocyte Biology2003，73，： | 1 |
| 10 | New categorization of human vascular endothelial cells by pro-vs anti-proliferative phenotypes显示文摘AIM: To integrally understand the effects of human vascular endothelial cells(VECs) on the proliferation of vascular smooth muscle cells(VSMCs).METHODS: Various kinds of human VECs of different origins were co-cultured with human aortic smooth muscle cells, a representative of human VSMCs. To exclude the irrelevant effects due to growth competition between VECs and VSMCs, the proliferation of VECs had previously been arrested via a low-dose gamma rayirradiation. To discriminately analyze the proliferation of VSMCs from that of VECs, the former cells were labeled with red fluorescent dye while the latter cells were labeled with green fluorescent dye before performing coculture experiments. After 4 d, total cells were harvested and subjected to flow cytometric analyses. Decrements in red fluorescence intensities due to proliferationmediated dilutions were measured and mathematically processed using a specific software to quantitatively evaluate the proliferation of VSMCs. The findings obtained from the flow cytometry-based analyses were further validated by microscopic observations. RESULTS: Commercially available primary cultured human VECs exclusively promoted VSMC proliferation regardless of their tissue origins and we termed these pro-proliferative VECs as 'typeⅠ'. By contrast, VECs freshly generated from human bone marrow-derived endothelial progenitors cells or human pluripotent stem cells including embryonic stem cells and induced pluripotent stem cells suppressed VSMC proliferation and we termed these anti-proliferative VECs as 'typeⅡ'. Repetitive subcultures as well as oxidative stress induced 'type Ⅱ VECs to typeⅠ' conversion along with an induction of Regulator of G-protein signaling 5(RGS5)Compatibly, anti-oxidant treatments suppressed both the subculture-dependent 'typeⅡ to typeⅠ' conversion and an induction of RGS5 gene. Immunostaining studies of clinical specimens indicated that RGS5 protein expressions in endothelial layers were low in norma arteries but they were up-regulated in pathologica arteries including hypertension, atherosclerosis and autoimmune vasculitis in a dose-dependent manner Overexpression and knockdown of RGS5 caused that'typeⅡ to typeⅠ' and 'typeⅠ to type Ⅱ' phenotype conversions of VECs, respectively. CONCLUSION: Human VECs are categorized into two types: pro-proliferative RGS5^(high) VECs(typeⅠ) and antiproliferative RGS5 ^(low) VECs(typeⅡ). | Miwako Nishio Masako Nakahara Chikako Sato Koichi Saeki Hidenori Akutsu Akihiro Umezawa Kazuyuki Tobe Kazuki Yasuda Akira Yuo Kumiko Saeki | 2015 | World Journal of Translational Medicine2015，4，3： | 1 |
| 11 | p38 mitogen-activated protein kinase regulates type-Ⅰ vs type-Ⅱ phenotyping of human vascular endothelial cells显示文摘AIM: To identify kinases involved in phenotype regulation of vascular endothelial cells(VECs): Proproliferative G-protein signaling 5(RGS5)^(high)(typeⅠ) vs anti-proliferative RGS5^(low)(typeⅡ) VECs.METHODS: Proteomic kinase assays were performed to identify the crucial kinase involved in the phenotype regulation of human VECs using typeⅠ VECs, which promotes the proliferation of human vascular smooth muscle cells(VSMCs), and typeⅡ VECs, which suppress the proliferation of human VSMCs. The assays were performed using multiple pairs of typeⅠ and typeⅡ VECs to obtain the least number of candidates. The involvement of the candidate kinases was verified by evaluating the effects of their specific inhibitors on the phenotype regulation of human VECs as well as the expression levels of regulator of RGS5, which is the causative gene for the 'typeⅡ to typeⅠ' phenotype conversion of human VECs. RESULTS: p38α mitogen-activated protein kinase(p38α MAPK) was the only kinase that showed distinctive activities between typeⅠ and typeⅡ VECs: p38α MAPK activities were low and high in type-Ⅰand typeⅡ VECs, respectively. We found that an enforced expression of RGS5 indeed lowered p38α MAPK activitiesin typeⅡ VECs. Furthermore, treatments with a p38α MAPK inhibitor nullified the anti-proliferative potential in typeⅡ VECs. Interestingly, MAPK inhibitor treatments enhanced the induction of RGS5 gene. Thus, there is a vicious cycle between 'RGS5 induction' and 'p38α MAPK inhibition', which can explain the unidirectional process in the stress-induced 'typeⅡ to typeⅠ' conversions of human VECs. To understand the upstream signaling of RGS5, which is known as an inhibitory molecule against the G protein-coupled receptor(GPCR)-mediated signaling, we examined the effects of RGS5 overexpression on the signaling events from sphingosine-1-phosphate(S1P) to N-cadherin, because S1 P receptors belong to the GPCR family gene and N-cadherin, one of their downstream effectors, is reportedly involved in the regulation of VEC-VSMC interactions. We found that RGS5 specifically bound with S1P1. Moreover, N-cadherin localization at intercellular junctions in typeⅡ VECs was abolished by 'RGS5 overexpression' and 'p38α MAPK inhibition'.CONCLUSION: p38α MAPK plays crucial roles in 'type-Ⅰ vs type-Ⅱ' phenotype regulations of human VECs at the downstream of RGS5. | Masako Nakahara Miwako Nishio Koichi Saeki Akira Yuo Kumiko Saeki | 2015 | World Journal of Translational Medicine2015，4，3： | 1 |
| 12 | Transcribed pseudogene 6PPM1K generates endogenous siRNA to suppress oncogenic cell growth in hepatocellular carcinoma 显示文摘 | Chan WL Yuo CY Yang WK | 2013 | Nucleic Acids Res2013，41，6： | 1 |
| 13 | Pro-vs anti-stenotic capacities of type-Ⅰ vs type-Ⅱ human induced pluripotent-derived endothelial cells显示文摘AIM:To verify in vivo relevance of the categorization of human vascular endothelial cells(VECs)into type-I(proproliferative)and type-II(anti-proliferative).METHODS:Endothelial layers of murine femoral arteries were removed by wire injury(WI)operation,a common technique to induce arteriostenosis.Type-I and type-II VECs produced from human induced pluripotent stem cells(iPSCs),whose characters were previously determined by their effects on the proliferation of vascular smooth muscle cells in in vitro co-culture experiments,were mixed with Matrigel?Matrix.The mixtures were injected into subcutaneous spaces around WI-operated femoral arteries for the transplanted human iPSC-derived VECs(iPSdECs)to take a route to the luminal surface via vasa vasorum,a nutrient microvessel for larger arteries.Histologies of the femoral arteries were examined over time.The presence of human iPSdECs was checked by immunostaining studies using an antibody that specifically recognizes human VECs.Degrees of stenosis of the femoral arteries were calculated after three weeks.To determine the optimal experimental condition,xenotransplantation experiments were performed under various conditions using immunocompromised mice as well as immunocompetent mice with or without administration of immunosuppressants.RESULTS:Because immunocompromised mice showed unexpected resistance to WI-induced arteriostenosis,we performed xenotransplantation experiments using immunocompetent mice along with immunosuppressant administrations.After one week,luminal surfaces of the WI-operated arteries were completely covered by human iPSdECs,showing the efficacy of our novel transplantation technique.After three weeks,type-IiPSdECs-transplanted arteries underwent total stenosis,while type-II-iPSdECs-transplanted arteries remained intact.However,untransplanted arteries of immunosuppressant-treated mice also remained intact by unknown reasons.We found that transplanted human VECs had already been replaced by murine endothelial cells by this time,indicating that a transient existence of human type-II-iPSdECs on arterial luminal surfaces can sufficiently prevent the development of stenosis.Thus,we re-performed xenotransplantation experiments using immunocompetent mice without administrating immunosuppressants and found that arteriostenosis was accelerated or prevented by transplantation of type-I or type-II iPSdECs,respectively.Similar results were obtained from the experiments using human embryonic stem cell-derived VECs at early passages(i.e.,type-II)and late passages(i.e.,type-I).CONCLUSION:Pro-and anti-stenosis capacities of type-I and type-II human iPSdECs were verified,respectively,promising a therapeutic application of allogenic iPSdECs. | Miwako Nishio Masako Nakahara Koichi Saeki Katsuhito Fujiu Hiroshi Iwata Ichiro Manabe Akira Yuo Kumiko Saeki | 2015 | World Journal of Translational Medicine2015，4，3： | 1 |
| 14 | Outcomes of endovas- cular intervention for May-Thurner syndrome显示文摘 | Hager ES Yuo T Tahara R | 2013 | J Vasc Surg Venous Lymphat Disord2013，1，3： | 1 |
| 15 | Cell cycle regulated phosphorylation of cAMP response element binding protein:identification novel phosphorylation sites显示文摘 | Saeki K Yuo A Takaku F | 1999 | Biochem J1999，338，1： | 1 |
| 16 | Local reduction of fold bifurcation relevant to voltage collapse in an electric power system显示文摘 | Cao Guo Yuo Chen Chen | 2002 | International Journal of Bifurcation and Chaos2002，12，5： | 1 |
| 17 | Distinct involvement of cAMP-response elementdependent transcriptions in functional and morphological maturation during retinoid-mediated human myeloid differentiation 显示文摘 | Saeki K Yuo A | 2003 | Journal of Leukocyte Biology2003，73，5： | 1 |
| 18 | Predicting the risk of lymph node involvement using the pre-treatment prostate specific antigen and Gleason score in men with clinically localized prostate cancer显示文摘 | Roach M 3rd Marquez C Yuo HS | 1994 | Int J Radiat Oncol Biol Phys1994，28，1： | 1 |
| 19 | Results on the Modified Riccati Equation: Target Tracking Applications 显示文摘 | Yuo B | 2006 | IEEE Trans AES2006，42，1： | 1 |
| 20 | Bcl-2 down-regulation causes autophagy in a caspase-independent manner in human leukemic HL60 cells显示文摘 | SAEKI K YUO A OKUMA E | 2000 | Cell Death Differ2000，7，12： | 1 |
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