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| 1 | Phylogeny and evolutionary history of the silkworm显示文摘The silkworm,Bombyx mori,played an important role in the old Silk Road that connected ancient Asia and Europe.However,to date,there have been few studies of the origins and domestication of this species using molecular methods.In this study,DNA sequences of mitochondrial and nuclear loci were used to infer the phylogeny and evolutionary history of the domesticated silkworm and its relatives.All of the phylogenetic analyses indicated a close relationship between the domesticated silkworm and the Chinese wild silkworm.Domestication was estimated to have occurred about 4100 years ago(ya),and the radiation of the different geographic strains of B.mori about 2000 ya.The Chinese wild silkworm and the Japanese wild silkworm split about 23600 ya.These estimates are in good agreement with the fossil evidence and historical records.In addition,we show that the domesticated silkworm experienced a population expansion around 1000 ya.The divergence times and the population dynamics of silkworms presented in this study will be useful for studies of lepidopteran phylogenetics,in the genetic analysis of domestic animals,and for understanding the spread of human civilizations. | SUN Wei YU HongSong SHEN YiHong BANNO Yutaka XIANG ZhongHuai ZHANG Ze | 2012 | Science China(Life Sciences)2012，55，6： | 6 |
| 2 | 蚕系统发生及进化历史分析显示文摘古丝绸之路是连接古亚洲和古欧洲的纽带.作为重要媒介,家蚕和丝绸在其中发挥着重要作用.家蚕是目前唯一一种被完全驯化的昆虫.然而,利用分子数据研究家蚕起源及进化历史的研究还很少,尤其是家蚕及其祖先野桑蚕的分化时间还有待确定.作为重要的驯化物种,家蚕的群体动力学变化历程也鲜有报道.本研究利用线粒体和核基因DNA序列推断了家蚕及其近缘物种的系统发生关系和进化历史.所有系统发生分析结果表明,家蚕和中国野桑蚕具有更近的亲缘关系.家蚕的驯化时间大约为4100年.中国野桑蚕大约在23600年前与日本野桑蚕分开,这个时间与化石证据和历史记载一致.另外,研究结果表明,家蚕在大约1000年前经历了群体扩张.本文对蚕的分化时间及蚕群体动力学进行研究,不仅有利于驯化动物的遗传分析和鳞翅目昆虫的系统发生分析,而且有利于理解人类文明的传播. | 孙伟 于红松 沈以红 Banno Yutaka 向仲怀 张泽 | 2012 | 中国科学:生命科学2012，42，6： | 4 |
| 3 | Study on fibroin heavy chain of the silkworm Bombyx mori by fluorescence in situ hybridization (FISH)显示文摘The sericulture industry plays a very important role in our national economy. Silkworm (Bombyx mori) is always regarded as a model animal and biological reactor. There have been detailed studies on the structure, expression and control and molecular evolution of silk genes. However, few, if any, reports are available on the localization of structural genes in silkworm by molecular cytogenetics. The present experiment has tentatively localized the Fib-H gene at the distal end of the 25th linkage group, namely at the 25-0.0 position, and verified that Fib-H has only one locus, thus providing a temporary solution to the problem about its localization. | 宋方洲 张平波 易发平 洪锡钧 鲁成 Yutaka Banno Hiroshi Fujii Katsumi Koga | 2002 | Science China(Life Sciences)2002，45，6： | 2 |
| 4 | Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization显示文摘The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper. | Yutaka BANNO Hiroshi FUJII | 2008 | Science China(Life Sciences)2008，51，2： | 2 |
| 5 | 家蚕丝胶基因1(Ser-1)和胰凝乳蛋白酶抑制因子13基因(CI-13)的FISH定位显示文摘应用荧光原位杂交技术对家蚕单拷贝的丝胶基因1(Ser-1)及胰凝乳蛋白酶抑制因子13基因(CI-13)进行了分子细胞遗传学的染色体定位.结果表明:Ser-1位于第11连锁群染色体的近端部位置,在粗线期染色体上的相对位置为12.5±1.4;CI-13位于第2连锁群染色体的近端部,在粗线期染色体上的相对位置为8.2±1.2,进而绘制了上述基因在家蚕染色体上的位置模式图—FISH图,并对家蚕染色体的荧光原位技术及其应用进行了探讨. | 宋方洲 常平安 张平波 易发平 鲁成 马永平 Banno Yutaka Fujii Hiroshi | 2008 | 中国科学(C辑)2008，38，2： | 1 |
| 6 | Purification, Characterization and Cloning of a Chymotrypsin Inhibitor (CI-9) from the Hemolymph of the Silkworm, Bombyx mori显示文摘 | Ping Zhao Qingyou Xia Juan Li Hiroshi Fujii Yutaka Banno Zhonghuai Xiang | 2007 | The Protein Journal2007，，5： | 1 |
| 7 | 石墨表层对四面体非晶炭膜中受激电子的石墨建序化作用显示文摘对滲气阴极真空电弧法制备的四面体非晶炭(ta-C)膜实施氧等离子体刻蚀,消除其表面石墨层后,发现:原沉积膜中ta-C石墨表层的消除会影响其受激电子的石墨建序化。应用发射电子能耗谱,表面增强拉曼光谱和表面敏化X光吸收光谱等测量方法,测定了其表层的消除(程度)。样品的氧等离子体刻蚀阻迟了受激电子的石墨化作用,可能归因于多相成核过程中石墨晶核的缺失之故. | 梁士金 Tatsuya Banno Yutaka Mera Masahiro Kitajima Kunie Ishioka Yoshihisa Harada Yoshinori Kitajima Shik Shin Koji Maeda | 2008 | 新型炭材料2008，23，3： | 0 |
| 8 | 家蚕肾脏形卵(ki)的荧光差异显示研究显示文摘为了探明家蚕(Bombyxmori )肾脏形卵的胚子不能正常发育的原因,使用FDD(flourescentdifferentdisplay)法对ki(kidney)基因同质型与异质型的母蛾所产未受精卵及受精24h卵的mRNA进行了差异显示研究。通过137组引物组合进行FDD差异显示,结果检出8254条扩增带。其中未受精卵与受精后24h卵之间的差异片段为206条,占总片段数的2.5%,而ki同质型与ki异质型的差异片段为141条,占总片段数的1.7%,其余的95.8%的扩增片段为4方共有,并且约60%的差异带是比较清晰的。回收了较明显的差异片段计199条,占总扩增带数的2.4%,并对其中部分差异片段进行序列分析表明,其中5个克隆序列分别为:smallheatshockproteins、proproteinconvertase、cancerantigenlikeprotein、fusionRNA-bindingprotein及receptorlikeproteinkinase同源序列,其余5个克隆序列未发现同源性,可能是新基因。 | 李斌 夏庆友 Hiroshi Fujii Yutaka Banno 鲁成 | 2002 | 农业生物技术学报2002，10，3： | 0 |
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