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| 1 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
| 2 | Tetrapod -shaped ZnO whisker and its composites 显示文摘 | ZHOU Z PENG W KE S | 1999 | J Materials Processing Technology1999，8990，： | 2 |
| 3 | 王红阳院士发现肝癌患者中红细胞增多症的分子机制显示文摘近日国际著名肝脏疾病杂志Hepatology在线发表了第二军医大学附属东方肝胆外科医院王红阳院士研究成果,癌细胞来源的促红细胞生成素在肝细胞癌的进展中发挥一定的作用,并且EPO/EPOR可被视为具有红细胞增多症的肝癌患者的一个治疗靶标。红细胞增多症(Erythrocytosis)与许多类型的肿瘤相关,包括肾细胞癌、脑膜瘤以及肝细胞癌(HCC)。 | Ke S Chen S Dong Z | 2016 | 胃肠病学和肝病学杂志2016，25，12： | 2 |
| 4 | Photocatalytic degradation of a cationic azo dye by TiO2/bentonite nanocomposite 显示文摘 | Sun Z S Chen Y X Ke Q | 2002 | J Photochem Photobiol A2002，149，13： | 1 |
| 5 | Characterization of an early gene encoding for dUTPase in Rana grylio virus 显示文摘 | Zhao Z Ke F Gui J F | 2007 | Virus Res2007，123，2： | 1 |
| 6 | Seawater pH sensor based on the long period grating in a single-mode-multimode-single-mode structure 显示文摘 | WANG Ke KLIMOV D KOLBER Z | 2009 | Optical Engineering2009，48，3： | 1 |
| 7 | Mechanical method determining precipitation in an ultra-low carbon bainitic steel显示文摘 | Z J Dang Y Zhang J Ke | 1988 | Journal of University of Science and Technology Beijing1988，5，3： | 1 |
| 8 | Nuclear targeting ofcyclin-dependent Kinase 2 reveals essential roles of cyclin-dependent Kinase 2 localization and Cyclin E in vitaminD-mediated growth inhibition显示文摘 | Flores 0 Wang Z Knudsen KE | 2010 | Endocrinology2010，151，3： | 1 |
| 9 | 查看详情显示文摘 | Wu D J Cao L Ke S Z | | 0，，： | 1 |
| 10 | Temporal and spatial distribution of dissolved oxygen in the Pearl River Estuary and adjacent coastal waters显示文摘 | YIN K LIN Z KE Z | 2004 | Continental Shelf Research2004，24，16： | 1 |
| 11 | Expression of DPC4/Smad4 in nonsmall-cell lung carcinoma and its relationship with angiogenesis 显示文摘 | KE Z ZHANG X MA L | 2008 | Neoplasma2008，55，4： | 1 |
| 12 | Prostaglandin E2 increases bone strength in intact rats and in ovariectomised rats with established osteopenia显示文摘 | Ke H Z Shen V W Qi H | 1998 | Bone1998，23，： | 1 |
| 13 | Characterization of an Early Gene Encoding for dUTPase in Rana grylio Virus显示文摘 | Zhao Z Ke F Gui J F | 2007 | Virus Research2007，123，： | 1 |
| 14 | New polyoxometalate- based mononuclear lanthanide complexes with slow relax- ation of magnetization 显示文摘 | Zhou W Z Feng X J Ke H S | 2012 | Inorganica Chimica Acta2012，394，： | 1 |
| 15 | Controlling factors of spring-summer phytoplankton succession in Taihu Lake (Meiliang Bay, China) 显示文摘 | KE Z XIE P GUO L | 2008 | Hydrobiologia2008，607，： | 1 |
| 16 | Characterization of an early gene encoding for dUTPase in Rana grylio virus 显示文摘 | ZHAO Z KE F GUI J F | 2007 | Virus Research2007，123，2： | 1 |
| 17 | Apoptotic effect of cyanobacterial extract on rat hepatocytes and human lymphocytes显示文摘 | Mankiewicz J Tarczynska M Fladmark KE Doskeland SO Walter Z Zalewski M | 2001 | Environ Toxicol2001，16，3： | 1 |
| 18 | The dimensional stability of a microcellular injection molded gear shaft 显示文摘 | Hwang S S Ke Z S | 2008 | International Communications in Heat and Mass Transfer2008，35，3： | 1 |
| 19 | An efficient ant colony optimization approach to attribute reduction in rough set theory显示文摘 | Ke LJ Feng Z R Ren Z G | 2008 | Pattern Recognition Letters2008，29，9： | 1 |
| 20 | 显示文摘 | Wang Z Y Han E H Ke W | 2007 | Corrosion Science2007，49，5： | 1 |
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