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| 1 | The novel gene BrMYB2,located on chromosome A07,with a short intron 1 controls the purple-head trait of Chinese cabbage(Brassica rapa L.)显示文摘Anthocyanins are important secondary metabolites in plants,but information on anthocyanin biosynthesis mechanisms in Chinese cabbage is limited.The new purple head Chinese cabbage cultivar 11S91 was analyzed,and an R2R3-MYB regulatory gene BrMYB2,located on chromosome A07,controlling the dominant purple-head trait was isolated.High expression of BrMYB2 generated a large accumulation of anthocyanins in 11S91,accompanied by highly upregulated BrTT8,BrF3′H,BrDFR1,BrANS1,BrUGTs,BrATs,and BrGSTs.11S91 inherited the purple locus from purple trait donor 95T2-5,and they shared consensus CDSs and gDNAs with those of BrMYB2(cBrMYB2 and gBrMYB2).Two SNPs in cBrMYB2 in 11S91 did not cause loss of function;in addition to several SNPs at both ends of intron 1,a large deletion had occurred in intron 1 of gBrMYB2 in 11S91.Genetic transformation of Arabidopsis showed that gBrMYB2 overexpression lines presented deeper purple color and higher expression than did the cBrMYB2 and cBrmyb2 lines,whereas gBrmyb2 with a long intron 1 did not cause the purple phenotype.We first show that BrMYB2 promotes anthocyanin biosynthesis under the control of the short intron 1 of gBrMYB2 in purple head Chinese cabbage,and gBrmyb2 with a long intron 1 represses anthocyanin production in white head Chinese cabbage.This evidence provides a new understanding of anthocyanin biosynthesis and purple germplasm generation in Brassica vegetables. | Qiong He Junqing Wu Yihua Xue Wenbin Zhao Ru Li Lugang Zhang | 2020 | Horticulture Research2020，7，1： | 4 |
| 2 | Genetic Analysis and Mapping of the Purple Gene in Purple Heading Chinese Cabbage显示文摘To analyze genetic linkage and map purple gene(Br Pur) controlling purple inner leaves trait of Chinese cabbage, a F2 population was constructed by selfing a F1 plant from homozygous purple heading line ‘14S839' and homozygous orange heading line ‘14S162'. The phenotype investigation of head color showed that the segregation ratio of purple to non-purple individuals was consistent with the expected ratio of 3:1,which indicated that purple inner leaves trait is controlled by a single dominant gene. A total of 297 SSRs in whole genome of Chinese cabbage were tested by modified Bulked Segregant Analysis(BSA) method. Two linked markers flanking Br Pur, A710 and A714, were obtained and Br Pur was mapped on linkage group A07 based on the sequence of these two markers. Subsequently, two new markers flanking Br Pur, CL-12 and B214-87, were developed based on two known anthocyanins-related genes, Br4CL3 and Bra004214. Genetic mapping of all markers in the F2 mapping population showed CL-12 and B214-87 linked to Br Pur with the genetic distance of 3.1 c M and 3.5 c M, respectively. | WU Junqing ZHAO Jing QIN Meiling REN Yanjing ZHANG Huamin DAI Zihui HAO Lingyu ZHANG Lugang | 2016 | Horticultural Plant Journal2016，2，6： | 3 |
| 3 | Enhanced ionic conductivity in a novel composite electrolyte based on Gd-doped SnO_(2) nanotubes for ultra-long-life all-solid-state lithium metal batteries显示文摘All-solid-state electrolytes are exceedingly attractive because of the outstanding inherent safety and energy density compared to liquid electrolytes.Whereas,it is still formidable to simultaneously design solid electrolytes with favorable electrode/electrolyte interface compatibility and high ionic conductivity in a simple and scalable manner.Hence,the oxygen-vacancy-rich Gd-doped SnO_(2) nanotubes(GDS NTs)are innovatively prepared and applied to the electrolyte of all-solid-state lithium metal batteries for the first time.The addition of GDS NTs can validly construct long-range co ntinuous ion transport networks in the poly(ethylene oxide)(PEO)-based system and greatly improve the mechanical properties of the electrolyte.Compared to the PEO-based electrolyte,the composite electrolyte displays a higher lithium ion conductivity of 2.41×10^(-4) S cm^(-1) at 30℃,a higher lithium ion transference number up to 0.62 and a wider electrochemical window of 5 V at 50℃.In addition,the composite electrolyte manifests outstanding compatibility with high-voltage LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NMC811)cathode,LiFePO4 cathode and lithium metal anode.The assembled Li/Li symmetric battery exhibits stable Li plating/stripping cycling performance,which can cycle steadily for 1500 h at a capacity of 0.3 mA h cm^(-2).And Li/LiFePO4 battery still maintains a high capacity of 131.54 mA h g^(-1) at 0.5C after 800 cycles,which has a superior capacity retention rate of 93.2%.The obtained novel composite electrolyte has promising application prospects in the field of all-solid-state lithium metal cells. | Lugang Zhang Nanping Deng Junbao Kang Xiaoxiao Wang Hongjing Gao Yarong Liu Hao Wang Gang Wang Bowen Cheng Weimin Kang | 2023 | Journal of Energy Chemistry2023，，2： | 0 |
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