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| 1 | High performance liquid chromatographic separation of thirteen drugs collected in Chinese Pharmacopoeia 2010(Ch.P2010) on cellulose ramification chiral stationary phase显示文摘The enantiomers separation of thirteen drugs collected in Ch.P2010 was performed on chiral stationary phase of cellulose ramification (chiralpak OD and chiralpak OJ) by high performance liquid chromatographic (HPLC) methods, which included ibuprofen (C1), ketoprofen (C2), nitrendipine (C3), nimodipine (C4), felodipine (C5), omeprazole (C6), praziquantel (C7), propranolol hydrochloride (C8), atenolol (C9), sulpiride (C10), clenbuterol hydrochloride (C11), verapamil hydrochloride (C12), and chlorphenamine maleate (C13). The mobile phase consisted of isopropanol and n-hexane. The detection wavelength was set at 254 nm and the fiow rate was 0.7 mL/min. The enantiomers separation of these thirteen racemates on chiralpak OD column and chiralpak OJ column was studied, while the effects of proportion of organic additives, alcohol displacer and temperature on the separation were studied. And the mechanism of some of racemates was discussed. The results indicated that thirteen chiral drugs could be separated onchiral stationary phase of cellulose ramification in normal phase chromatographic system. The chromatographic retention and resolution of enantiomers could be adjusted by factors including column temperature and the concentration of alcohol displacer and organic alkaline modifier in mobile phase. It was shown that the resolution was improved with reducing concentration of alcohol displacer. When concentration of organic alkaline modifier was 0.2% (v/v), the resolution and the peak shape were fairly good. Most racemates mentioned above had better resolution at column temperature of 25 1C. When racemates were separated, the temperature should be kept so as to obtain stable separation results. | Ying Zhoua,b, Chao Maa, Yan Wanga, Qi-Ming Zhangb, Yi-Ying Zhanga, Jie Fua, Hong Gaoc, Li-Xun Zhaoc aState Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China bNational Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China cInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China | 2012 | Journal of Pharmaceutical Analysis2012，2，1： | 4 |
| 2 | Investigation on characteristics of leachate and concentrated leachate in three landfill leachate treat- ment plants显示文摘 | Zhang Q Q Tiana B H Zhanga X | 2013 | Waste Management2013，33，11： | 1 |
| 3 | Development of a 3-D thermal model of the low-pressure diecast (LPDC) process of A356 aluminum alloy wheels 显示文摘 | ZHANGA B MAIJIR D M COCKCROFT S L | 2007 | Materials Science and Engineering: A2007，464，12： | 1 |
| 4 | Identification and character- ization of a novel protective antigen, enolase of Streptococcus suis serotype 2显示文摘 | ZhangA Chen B Mu X | 2009 | Vaccine2009，27，9： | 1 |
| 5 | Effect of the molecular interaction on molecular packing and orientation in azobenzene-functionalized self-assembled monolayers on gold显示文摘 | Zhanga J Zhao B A Hang H L | 1998 | Thin Solid Films1998，，： | 1 |
| 6 | Role of Notch expression in premature senescence of murine bone marrow stromal cells显示文摘The aim of the present study was to investigate the role of the Notch signaling pathway in premature senescence of murine bone marrow stromal cells in vitro. The intracellular domain of Notch 1 (ICN) was transfected into cultured murine bone marrow stromal cells by lipofectamine transfection. After three days, the proliferation of transfected cells was measured by MTT assay. Cell cycle distribution was analyzed by flow cytometry. Senescence-associated beta-galactosidase (SA-beta-gal) was measured, and the percentage of positive cells was evaluated by assessing 1000 cells in random fields of view. The expressions of p53 and p21Cip1/Waf1 were analyzed by both RT-PCR and Western blot analysis. The results showed that activation of Notch signaling inhibited proliferation of murine bone marrow stromal cells with induction of G1 arrest, increased the percentage of SA-beta-gal positive cells, and upregulated p53 and p21Cip1/Waf1 mRNA and protein expression levels. Thus, the activated Notch signaling could induce premature senescence of bone marrow stromal cells through the p53-p21Cip1/Waf1 pathway. | Kejie Zhanga,b, Lifang Huang b, Hanying Sunb, Yan Zhub, Yi Xiaob, Mei Huang b, Wenli Liub,a Department of Hematology, Zhongshan Hospital, Xiamen University, Fujian Medical University Clinic Teaching Hospital, Xiamen 361004, China b Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2009 | Progress in Natural Science:Materials International2009，19，5： | 1 |
| 7 | Develop-ment of eolour of broccoli heads as affected by controlled at- mosphere storage and temperature显示文摘 | Schoutena R E Zhanga X B Verschoor J A | 2009 | Postharvest Biology and Technology2009，51，1： | 1 |
| 8 | An experimental in- vestigation of the heat losses of a U - type solar heat pipe receiver of a parabolic trough collector - based natural circulation steam generation system显示文摘 | Liang Zhanga Zitao Yua Liwu Far a b elt | 2013 | Renewable Energy2013，57，： | 1 |
| 9 | Direct evidence that suspensor cells have embryogenic potential that is suppressed by the embryo proper during normal embryogenesis显示文摘 | Yuan Liua b Xinbo Lia Jing Zhaoa Xingchun Tanga c Shujuan Tiana Junyi Chena Ce Shia Wei Wanga Liyao Zhanga Xianzhong Fengb and Meng-Xiang Suna 1 | 2015 | PNAS2015，，： | 1 |
| 10 | Phylloquinone (Vitamin K1) Content of Vegetables显示文摘 | Damona M Zhanga N Z Haytowitzb D B Boot ha S L | 2005 | Journal of Food Composition and Analysis2005，18，11： | 1 |
| 11 | Optimized al- gorithms for predictive range and kNN queries on moving objects 显示文摘 | Zhanga R Jagadishb H V Daic B T | 2010 | Information Systems2010，35，8： | 1 |
| 12 | Development of a universal phosphorylated peptide-binding protein for simultaneous assay of kinases显示文摘This study describes the development of a universal phosphorylated peptide-binding protein designed to simultaneously detect serine, threonine and tyrosine kinases. The Escherichia | Wei Lia,b,d,1, Lijun Bib,1, Wenhua Wangb,d,1, Yongjin Lia, Yafeng Zhoua, Hongping Weia, Tao Jiangb, Lin Baib,d, Yuanyuan Chenb, Zhiping Zhanga, Xinghua Yuanc, Jianping Xiaoc, Xian-En Zhanga, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China | 2009 | 生物物理学报2009，25，S1： | 0 |
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