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| 1 | Pioglitazone ameliorates neuronal damage after traumatic brain injury via the PPARg/NF-kB/IL-6 signaling pathway显示文摘Traumatic brain injury(TBI)is the major cause of high mortality and disability rates worldwide.Pioglitazone is an activator of peroxisome proliferator-activated receptor-gamma(PPARγ)that can reduce inflammation following TBI.Clinically,neuroinflammation after TBI lacks effective treatment.Although there are many studies on PPARγin TBI animals,only few could be converted into clinical,since TBI mechanisms in humans and animals are not completely consistent.The present study,provided a potential theoretical basis and therapeutic target for neuroinflammation treatment after TBI.First,we detected interleukin-6(IL-6),nitric oxide(NO)and Caspase-3 in TBI clinical specimens,confirming a presence of a high expression of inflammatory factors.Western blot(WB),quantitative real-time PCR(qRTPCR)and immunohistochemistry(IHC)were used to detect PPARγ,IL-6,and p-NF-kB to identify the mechanisms of neuroinflammation.Then,in the rat TBI model,neurobehavioral and cerebral edema levels were investigated after intervention with pioglitazone(PPARγactivator)or T0070907(PPARγinhibitor),and PPARγ,IL-6 and p-NF-kB were detected again by qRT-PCR,WB and immunofluorescence(IF).The obtained results revealed that:1)increased expression of IL-6,NO and Caspase-3 in serum and cerebrospinal fluid in patients after TBI,and decreased PPARγin brain tissue;2)pioglitazone could improve neurobehavioral and reduce brain edema in rats after TBI;3)the protective effect of pioglitazone was achieved by activating PPARγand reducing NF-kB and IL-6.The neuroprotective effect of pioglitazone on TBI was mediated through the PPARγ/NF-kB/IL-6 pathway. | Yongbing Deng Xue Jiang Xiaoyan Deng Hong Chen Jie Xu Zhaosi Zhang Geli Liu Zhu Yong Chengfu Yuan Xiaochuan Sun Changdong Wang | 2020 | Genes & Diseases2020，7，2： | 6 |
| 2 | PTEN and AKT/GSK-3β/CRMP-2 signaling pathway are involved in neuronal apoptosis and axonal injury in early brain injury after SAH in rats显示文摘In early brain injury(EBI)after subarachnoid hemorrhage(SAH),white matter(WM)axonal injury plays a key role in the prognosis of the disease.The purpose of this study was to investigate the effects of phosphatase and tensin homolog deleted on chromosome ten(PTEN)on axonal injury and neuronal apoptosis post-SAH in rats and to find its underlying mechanism.Adeno-associated virus was injected into the lateral ventricle to suppress or promote PTEN.Neural function post-SAH in animals was determined by the modified Garcia score,beam balance,and Rotarod test,and the blood–brain barrier disruption was assessed by the brain water content.Axonal injury post-SAH was observed by TEM and determined by IF,and neuron apoptosis was measured by TUNEL staining.The mechanism was analyzed by Western blot to detect p-PTEN/PTEN,p-AKT/AKT,p-GSK-3β/GSK-3β,p-CRMP-2/CRMP-2,axonal injury markerβ-APP and pro-and anti-apoptosis proteins,including Bax and Bcl-2,expression.We found 1.After knocking down PTEN,neuronal apoptosis and axonal injury were alleviated,and nerve function and blood–brain barrier were protected;accordingly,after overexpression of PTEN,neuronal apoptosis and axon damage were aggravated,and nerve function damage and blood–brain barrier damage were increased.2.PTEN and AKT/GSK-3β/CRMP-2 pathway were jointly involved in regulating neuronal apoptosis and WM axon injury after SAH.According to our research,PTEN was a negative factor of EBI,and together with the AKT/GSK-3β/CRMP-2 signaling pathway aggravates neuronal apoptosis and WM axon damage after SAH.Inhibition of PTEN expression may become a new target for SAH treatment. | Hong Chen Chao Zhou Jianfeng Zheng Zhaosi Zhang Yongbing Deng Chongjie Cheng Zongduo Guo Gang Huo Cheng Yin Xiaochuan Sun | 2022 | Genes & Diseases2022，9，1： | 1 |
| 3 | Novel structure of AAO filmfabricatedbyconstantcurrentanodization显示文摘 | ZHAOSY CHANK YELONA | | 0，，： | 1 |
| 4 | Analyticalprogressoftraceelementsinchinesetradi-tionalmedicineusingatomicabsorptionspectrometry显示文摘 | YangHF(杨惠芳) ZhaoSY(赵淑英) WangZH(王朝晖) | | 陕西师范大学学报自科版0，，： | 1 |
| 5 | Synthesis,characterization,andbiologicalapplicationofsizecontrollednanocrystallineNaYF4:Yb,Erinfraredtovisibleupconversionphosphor显示文摘 | YIGS LUHC ZHAOSY etal | 2004 | NanoLett2004，，4： | 1 |
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