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		题名	作者	年代	出处	被引量
	1	Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35显示文摘A transformed cell line was constructed fromMythimna separata cells Ms7311 by lipofection methodTMs7311 cells were generated using a double selection tech-nique involving a selection in the antibiotic Zeocin, followedby a second round of selection by exhibiting cell characteri-zation. A cell clone expressing p35 was obtained with highlevel of AcMNPV and recombinant proteins. Compared withwild type Ms7311 cells, the cell clone showed increased resis-tance to Actinamycin D-induced apoptosis and a profoundresistance to nutrient development (PBS). When the cellclone was infected with recombinant baculoviruses express-ing secreted alkaline phosphatase (SEAP) and β-galactosi-dase, expression of the recombinant proteins from TMs7311cells exceeded that from parental Ms7311 cells. Production ofbudded virus and occlusion body was significantly higherthan that from parental cells Ms7311.	ZHENG Gulling LI Changyou LI Guoxun WANG Ping Robert R. Granados	2005	Chinese Science Bulletin2005，50，23：	6

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