| 4 | Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures显示文摘Objective: The medicinal mushroom Sanghuangporus vaninii produces pharmaceutically valuable hispidin polyphenols in natural habitats. However, due to the slow growth in nature, S. vaninii grown in the field(sclerotia) is not reliable for pharmaceutical purposes. Although higher biomass of fungal mycelia can be obtained in submerged cultures, the accumulation of hispidin polyphenols is rare.Methods: In this study, the polyunsaturated fatty acids(PUFAs), linoleic acid(LA), linolenic acid(ALA),and methyl jasmonate(Me Ja) were employed as the stimulant agents to coordinate the accumulation of biomass and hispidin polyphenols in its submerged cultures.Results: The addition of LA and ALA promoted the mycelial accumulation, while the addition of Me Ja inhibited the growth of S. vaninii concomitant with reduced total polyphenols. UPLC-Triple-TOF-MS analysis revealed an increased production of hispidin, phellinstatin, pinnilidine, and its derivatives upon the addition of LA and ALA, and hypholomine B and its isomer, 3,14’-bihispidinyl, and phelligridin E upon the addition of Me Ja on day 13. Intriguingly, total polyphenols from the Me Ja-supplementing cultures harbored a high capacity in scavenging free radicals. Chemical structural analysis showed that hispidin polyphenols had higher antioxidant activity due to more hispidin moieties induced by Me Ja.Conclusion: The supplement of PUFAs affects the synthesis and composition of hispidin polyphenols in S.vaninii. Our results provide a possibility to coordinate the production of hispidin polyphenols via submerged cultures of S. vaninii. | Wenwen Yuan Weihua Yuan Rong Zhou Guoying Lv Mengni Sun Yanxia Zhao Weifa Zheng | 2023 | Chinese Herbal Medicines2023，15，4： | 0 |
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