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| 1 | CryoEM structure of the tegumented capsid of Epstein-Barr virus显示文摘Epstein-Barr virus(EBV)is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies.Here,we present a complete atomic model of EBV,including the icosahedral capsid,the dodecameric portal and the capsid-associated tegument complex(CATC).Our in situ portal from the tegumented capsid adopts a closed conformation with its channel valve holding the terminal viral DNA and with its crown region firmly engaged by three layers of ring-like dsDNA,which,together with the penton flexibility,effectively alleviates the capsid inner pressure placed on the portal cap.In contrast,the CATCs,through binding to the flexible penton vertices in a stoichiometric manner,accurately increase the inner capsid pressure to facilitate the pressure-driven genome delivery.Together,our results provide important insights into the mechanism by which the EBV capsid,portal,packaged genome and the CATCs coordinately achieve a pressure balance to simultaneously benefit both viral genome retention and ejection. | Zhihai Li Xiao Zhang Lili Dong Jingjing Pang Miao Xu Qian Zhong Mu-Sheng Zeng Xuekui Yu | 2020 | Cell Research2020，30，10： | 1 |
| 2 | Eff ects of temperature on photosynthetic performance and nitrate reductase activity in vivo assay in Gracilariopsis lemaneiformis (Rhodophyta)显示文摘Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in China at present.After being acclimated to diff erent growth temperatures(15,20,25,and 30°C)for 7 days,the relative growth rate(RGR),nitrate reductase activity,soluble protein content and chlorophyll a fl uorescence of G.lemaneiformis were examined.Results show that RGR was markedly aff ected by temperature especially at 20°C at which G.lemaneiformis exhibited the highest eff ective quantum yield of PSII[Y(II)]and lightsaturated electron transport rate(ETR max),but the lowest non-photochemical quenching.Irrespective of growth temperature,the nitrate reductase activity increased with the incubation temperature from 15 to 30°C.In addition,the greatest nitrate reductase activity was found in the thalli grown at 20°C.The value of temperature coeffi cient Q10 of alga cultured in 15°C was the greatest among those of other temperatures tested.Results indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity,and the relationship among growth,photosynthesis,and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature. | Zhihai ZHONG Zhengyi LIU Longchuan ZHUANG Wanlin SONG Weizhou CHEN | 2021 | Journal of Oceanology and Limnology2021，39，1： | 1 |
| 3 | Effects of Temperature and Light on Growth Rate and Photosynthetic Characteristics of Sargassum horneri显示文摘The changing environmental factors exerted great influences on coastal macroalgal communities.To study the responses of the brown seaweed Sargassum horneri to temperature and light,S.horneri was cultured under three temperatures(20,25 and 30℃)and three light intensities(30,60,and 120μmol photons m-2 s-1)for seven days.The growth rate,chlorophyll a(Chl a)and carotenoids(Car)contents,chlorophyll fluorescence,and photosynthetic oxygen evolution rate were measured.The results show that the highest relative growth rate(RGR),maximal electron transport rate(rETRmax);the net photosynthetic rate(Pn)were observed at the lowest temperature(20℃)and highest light intensity(120μmol photons m-2 s-1);and the RGR and Pn were significantly inhibited by the highest temperature(30℃),especially at the lowest light intensity(30μmol photons m-2 s-1)(P<0.05).Additionally,the highest light intensity enhanced the non-photochemical quenching(NPQ)even under the highest temperature(30℃),indicating that the higher light intensity could induce photo-protection reaction of thalli.These results suggest that the higher temperature and lower light intensity exerted negative influences on S.horneri. | WANG Yong ZHONG Zhihai QIN Song LI Jialin LI Jingjing LIU Zhengyi | 2021 | Journal of Ocean University of China2021，20，1： | 1 |
| 4 | Comparison of the photo-acclimation potential of fl oating and benthic thalli of Sargassum horneri(Phaeophyta)during autumn and winter显示文摘Sargassum horneri is a foundational species and an important contributor to the fl oating seaweed stock along the northeastern coast of Asia.In this study,benthic and fl oating thalli of S.horneri were collected from Changdao Island(37°54′N,120°43′E),Bohai Bay,China.We conducted an in-situ and an indoor experiment to study the acclimation potential in S.horneri to abiotic conditions at sea surface in autumn and winter.Both benthic and fl oating thalli were cultured in situ for two months(from October to December)at diff erent depths:0 m above sea level(masl)and 3 m below sea level(mbsl),and their growth rate,biochemical content,and photosynthetic performance were compared.During the fi rst month of culture,the relative growth rate of fl oating thalli was 2-fold greater than that of benthic thalli at 0 masl.The photosynthetic rate of most thalli was signifi cantly higher at 0 masl than at 3 mbsl.In the indoor experiments,fl oating and benthic thalli were exposed to high light intensity(400μmol photons/(m^(2)·s)photosynthetically active radiation(PAR))for 21 d,and their photo-acclimation capacities were compared.Under high light intensity,the two types of thalli showed low maximum quantum yield(F_(v)/F_(m))and light utilisation effi ciency(α)but high light saturation point(E_(k)).Floating thalli showed higher photosynthetic rate and photoprotective ability than benthic thalli at high light intensity.The eff ective quantum yield of photosystem II[Y(II)]of both types of thalli recovered after a 6-day treatment with low light intensity(40μmol photons/(m^(2)·s)).These fi ndings suggest that S.horneri is highly acclimated to the sea surface environment,which possibly contributes to its rapid accumulation and long free-fl oating periods at the sea surface. | Jingjing LI Yunlong PANG Song QIN Zhengyi LIU Zhihai ZHONG Wanlin SONG Longchuan ZHUANG | 2022 | Journal of Oceanology and Limnology2022，40，1： | 0 |
| 5 | A Prospective, Randomized, Controlled Clinical Trial of the Effects of Ursodeoxycholic Acid in Alleviating Ischemia/ Reperfusion Injury in Liver Transplantation显示文摘 | Shuyun Wang Huamei Tang Zhihai Peng Guoqiang Qiu Guoqing Chen Junming Xu Lin Zhong | 2008 | 器官移植内科学杂志2008，3，2： | 0 |
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