|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction. | Xiao B Jing B Zhang YL Zhou DY Zhang WD | 2000 | World Journal of Gastroenterology2000，6，1： | 11 |
| 2 | Identification of SNP-containing regulatory motifs in the myelodysplastic syndromes model using SNP arrays and gene expression arrays显示文摘Myelodysplastic syndromes have increased in frequency and incidence in the American population, but patient prognosis has not significantly improved over the last decade. Such improvements could be realized if biomarkers for accurate diagnosis and prognostic stratification were successfully identified. In this study, we propose a method that associates two state-of-the-art array technologies-single nucleotide polymorphism (SNP) array and gene expression array-with gene motifs considered transcription factor -binding sites (TFBS). We are particularly interested in SNP-containing motifs introduced by genetic variation and mutation as TFBS. The potential regulation of SNP-containing motifs affects only when certain mutations occur. These motifs can be identified from a group of co-expressed genes with copy number variation. Then, we used a sliding window to identify motif candidates near SNPs on gene sequences. The candidates were filtered by coarse thresholding and fine statistical testing. Using the regression-based LARS-EN algorithm and a level-wise sequence combination procedure, we identified 28 SNP-containing motifs as candidate TFBS. We confirmed 21 of the 28 motifs with ChIP-chip fragments in the TRANSFAC database. Another six motifs were validated by TRANSFAC via searching binding fragments on coregulated genes. The identified motifs and their location genes can be considered potential biomarkers for myelodysplastic syndromes. Thus, our proposed method, a novel strategy for associating two data categories, is capable of integrating information from different sources to identify reliable candidate regulatory SNP-containing motifs introduced by genetic variation and mutation. | Jing Fan Jennifer G. Dy Chung-Che Chang Xiaobo Zhou | 2013 | Chinese Journal of Cancer2013，32，4： | 2 |
| 3 | Sleep deprivation by the 'Flower Pot' technique and spatial reference memory显示文摘 | Bradley DY Zhou Jun Smagin GN | 1997 | Physiol Behav1997，61，： | 1 |
| 4 | Association between obesity indices and blood pressure or hypertension:which index isthe best? 显示文摘 | Zhou ZQ Hu DY Chen J | 2009 | Public Health Nutr2009，12，8： | 1 |
| 5 | Proanthocyanidin from grape seed extract inhibits airway inflammation andremodeling in a mu- fine model of chronic asthma 显示文摘 | Zhou DY Fang SR Zou CF | 2015 | Nat Prod Commun2015，10，2： | 1 |
| 6 | Association between ad- mission hypoglycaemia and in - hospital and 3 - year mortality in older patients with acute myocardial infarction 显示文摘 | Yang SW Zhou YJ Hu DY | 2010 | Heart2010，96，18： | 1 |
| 7 | Hepatitis C virus core protein-induced loss of LZIP function correlates with cellular transformation 显示文摘 | Jin DY Wang HL Zhou Y | 2000 | EMBO J2000，19，4： | 1 |
| 8 | DNA-dependent protein kinase catalytic subunit modulates the stability of c-Myc oncoprotein显示文摘 | An J Yang DY Zhou PK | 2008 | Mol Cancer2008，7，32： | 1 |
| 9 | An automated pipeline for dendrite spine detection and tracking of 3D optical microscopy neuron images of in vivo mouse models 显示文摘 | FAN Jiang ZHOU Xiao-bo DY G | 2009 | Neuroinformatics2009，7，2： | 1 |
| 10 | Identification of O-digly-cosyl flavanones in Fructus aurantii by liquid chromatog-raphy with electrospray ioniztion and collision-induced dissociation mass spectrometry显示文摘 | Zhou DY Xu Q Xue XY | 2006 | J Pharma Biomed A-nal2006，42，： | 1 |
| 11 | Efficiency of ginkgo biloba extract (EGb751) in antioxidant protection against myocardial ischemia and reperfucion injury显示文摘 | Shen JG Zhou DY | 1995 | Biochem Mof Biol Int1995，35，： | 1 |
| 12 | Preparation and antioxidant activity of enzymatic hydrolysates from purple sea urchin ( Strongylocentrotus nudus ) gonad 显示文摘 | Qin L Zhu BW Zhou DY | 2011 | LWT- Food Sci Technol2011，44，4： | 1 |
| 13 | An automated pipeline for dendrite spine detection and tracking of 3D optical microscopy neuron images of in vivo mouse models 显示文摘 | FAN Jing ZHOU Xiaobo Dy G J | 2009 | Neuroinformatics2009，7，2： | 1 |
| 14 | Protective effects of apocynin on 'two-hit' injury induced by hemorrhagic shock and lipopolysaccharide显示文摘 | Zhou R Hu DY Liu LM | 2002 | J Acta Pharmacol Sin2002，23，11： | 1 |
| 15 | Composite en-ergy storage system involving battery and ultracapacitorwith dynamic energy management in microgrid applica-tions显示文摘 | Zhou Haihua Bhattacharya T Tran Dy | 2011 | IEEE Transactions on Power Electronics2011，26，3： | 1 |
| 16 | Biological features of novel avian influenza A (H7N9) virus显示文摘 | Zhou JF Wang DY Gao RB | 2013 | Nature2013，499，7459： | 1 |
| 17 | Identification of O-digly- cosyl flavanones in Fructus aurantii by liquid chromatogra- phy with eleetrospray ionization and collision-induced dis- sociation mass spectrometry显示文摘 | Zhou DY Xu Q Xue XY | 2006 | Journal of pharmaceutical and biomedical analysis2006，42，4： | 1 |
| 18 | Efficiency of Ginkgo biloba extract (EGb761 ) in anfioxidant protection against myocardial ischemia and reperfusion injury 显示文摘 | Shen JG Zhou DY | 1995 | Biochem Mol Bid Int1995，35，： | 1 |
| 19 | Efficiency of Ginkgo biloba extract(Egb)in antioxidant protection against myocardial ischemia and reperfusion injury 显示文摘 | Shen JG Zhou DY | 1995 | Biochem Mol Biol Int1995，35，1： | 1 |
| 20 | Rapid qualitative and quantitative analyses of flavanone aglycones in Fructus aurantii by HPLC ion-trap MS 显示文摘 | Zhou DY Xu Q Xue XY | 2007 | Journal of separation science2007，30，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员