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| 1 | Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression显示文摘AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10-4mol/L-10-7mol/L SA-A for 22 h.The cell viability wasassayed by[3H]proline incorporation,cellproliferation by[3H]TdR incorporation,cellcollagen synthetic rate was measured with[3H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α(1)I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10-4mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10-5mol/L-10-7mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10-6mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10-5mol/L-10-6mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10-5mol/L and10-6mol/L SA-A could decrease α(1)I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug. | Liu CH Hu YY Wang XL Liu P Xu LM | 2000 | World Journal of Gastroenterology2000，6，3： | 25 |
| 2 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 3 | Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level显示文摘 | Liu, LM Li, Y Zhu, Y Du, GC Chert, J | 2007 | 中国生物学文摘2007，21，10： | 12 |
| 4 | 急性缺血性脑卒中后低脉压与不良预后相关显示文摘众所周知,脉压与心脏功能、动脉僵硬度、体液状态和血管事件有关。该研究旨在探讨基于中国台湾地区脑卒中登记资料的急性缺血性脑卒中(acute ischemic stroke,AIS)患者入院时脉压的预后作用。 | 刘莉 叶鹏 Tang SC Yin JH Liu CH Sun MH Lee JT Sun Y Hsu CS Sun MC Lin CH Chen CH Lien LM Muo CH Jeng JS Hsu CY | 2017 | 中华高血压杂志2017，25，7： | 5 |
| 5 | Study and Application of Polarographic Catalytic Wave of Chlordiazepoxide in the Presence of Persulfate显示文摘Polarographic catalytic wave of chlordiazepoxide in the presence of K2S2O8 was studied in aqueous and DMF/H2O mixed solutions,The results showed that a single reduction wave in alkaline medium was the reduction of the N=C bond in 1,2-position of chlordiazepoxide via an intermediate free radical in two one-electron successive additions,When K2S2O8 was present,the free radical of the N=C bond was oxidized to regenerate the original,producing a parallel catalytic wave of chlordiazepoxide,It was determined that the apparent rate constant kf of the oxidation reaction was 3.2×10^3 mol^-1.L.s^-1,Using the catalytic wave the trace of chlordiazepoxide can be determined by linear-potential scan polarography,In NH3/NH4Cl(pH 10.2±0.1,0.12mol/L)/K2S2O8(0.016mol/L)supporting electrolyte,the second-order derivative peak current of the catalytic wave was rectilinear to chlordiazepoxide concentration in the range of 3.20×10^-8-1.60×10^-7,1.60×10^-7-1.44×10^-6 and 1.44×10^-6-1.44×10^-5mol/L,respectively.The limit of detection was 9.0×10^-9mol/L. | Guo, W Lin, H Liu, LM Guo, ZA Song, JF | 2002 | Chinese Journal of Chemistry2002，20，5： | 3 |
| 6 | Systemic infusion of FLKI(+) mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice显示文摘 | Fang,BJ Shi,MX Liao,LM Yang,SG Liu,YH Zhao,RC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 7 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 8 | Thalidomide attenuates tumor growth and preserves fast-twitch skeletal muscle fibers in cholangiocarcinoma rats显示文摘 | Liu KH Liao LM RO LS | 2008 | Surgery2008，143，3： | 1 |
| 9 | European Polymer Journal显示文摘 | Tang EJ Liu H Sun LM | 2007 | (10):4 210-4 2182007，，10： | 1 |
| 10 | Different distribution of breast can-cer subtypes in breast ductal carcinoma in situ (DCIS), DCIS withmicroinvasion, and DCIS with invasion component显示文摘 | Yu KD Wu LM Liu GY | 2011 | Ann Surg On-col2011，18，5： | 1 |
| 11 | Mechanisms of Rho kinase regulation of vascular reactivity following hemorrhagic shock in rats显示文摘 | Li T Liu LM Ming J | 2008 | Shock2008，29，1： | 1 |
| 12 | Comparative analysis of a large dataset indicates that internal transcribed spacer(ITS)should be incorporated into the core barcode for seed plants显示文摘 | Li DZ Gao LM Li H Wang H Ge X J Liu JQ Chen ZD Zhou SL Chen SL Yang JB | | 0，，： | 1 |
| 13 | δ opioid receptor antagonist, ICI 174,864, is suitable for the early treatment of uncontrolled hemorrhagic shock in rats 显示文摘 | Liu LM Tian KL Zhu Y | 2013 | Anesthesiology2013，119，2： | 1 |
| 14 | Role of angiogenesis inhibitors in cancer treatment 显示文摘 | Ellis LM Liu W Fan F Et al | 2001 | Oncology ( Williston Park)2001，15，7： | 1 |
| 15 | Comparison of the clinical value of multi-band mucosectomy versus endoscopic mucosal resec- tion for the treatment of patients with early-stage esophageal cancer 显示文摘 | Chen ZY Yang YC Liu LM | 2015 | Oncol Lett2015，9，6： | 1 |
| 16 | Hemorrhage induced vascular hyporeactivity to norepinephrine in select vasculatures of rats and the roles of nitric oxide and endothelin显示文摘 | Liu LM Ward JA Dubick MA | 2003 | Shock2003，19，： | 1 |
| 17 | Regulation of the transcription factor NF-K BI by microRNA-9 in human gastric adenocarcinoma 显示文摘 | Wan HY Guo LM Liu T | 2010 | Molecular Cancer2010，916，： | 1 |
| 18 | Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits显示文摘 | Tong XY Porter LM Liu GX | 2006 | Am J Physiol Heart Circ Physiol2006，291，： | 1 |
| 19 | Vascular endothelial growth factor in human colon cancer: biology and therapeutic implications显示文摘 | Ellis LM Tadahashi Y Liu W | 2000 | Oncologist2000，5，1： | 1 |
| 20 | Coordinated replenishments in inventory systems with correlated demands 显示文摘 | Liu LM Yuan XM | 2000 | European Journal of Operational Research2000，123，3： | 1 |
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