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| 1 | Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis(Coss. & Dur.) Maire显示文摘Objective:To investigate the chemical composition of a moderately polar extract(CHC1_3 soluble part of the MeOH-H_2O extract) obtained from the aerial parts(leaves and flowers) of Centaurea diluta Ait.subsp.algeriensis(Coss.& Dur.) Maire,a species endemic to Algeria and Morocco on which no reports are available to date.To evaluate in vitro the cytotoxic,antifungal and antimicrobial activities of this extract and the cytotoxic and antimicrobial activities of its isolated secondary metabolites.Methods:The cytotoxic effects of the extract were investigated on 3 human cancer cell lines i.e.the A549 non-small-cell lung carcinoma(NSCLC),the MCF7 breast adenocarcinoma and the U373 glioblastoma using a MTT colorimetric assay.Biological data allowed to guide the fractionation of the extract by separation and purification on silica gel 60(CC and TLC).The isolated compounds which were characterized by spectral analysis,mainly HR-ESIMS,HR-EIMS,UV and NMR experiments(~1H,^(13)C,COSY,ROESY,HSQC and HMBC) and comparison of their spectroscopic data with those reported in the literature,were evaluated for cytotoxic activities on six cancer cell lines(A549,MCF7,U373,Hs683 human glioma,PC3 human prostate and B16-F10 murine melanoma).The direct and indirect antibacterial and antifungal activities were determined using microdilution methods for the raw extract and TLC-bioautography and microdilution methods against standard and clinical strains for the isolated compounds.Results:The raw extract reduced cell viability with IC_(50)s of 27,25 and 21 μg/mL on A549,MCF7 and U373,respectively.Five secondary metabolites:two phenolic compounds(vanillin 1,paridol 3),a lignan[(-)-arctigenin 2]and two flavonoid aglycones(eupatilin 4 and jaceosidin 5),were then isolated from this extract.Moderate cytotoxic effects were observed for(-)-arctigenin 2(IC_(50)s:28 and 33μM on Hs683 and B16-F10,respectively),eupatilin 4(IC_(50)s:33 and 47 μM on B16-F10 and PC3,respectively) and jaceosidin 5(IC_(50)s:32 and 40 μM on PC3 and B16-F10,respectively).Conclusions:All the isolated compounds were described for the first time from this species.Although inactive against 7 tested microorganisms(fungi,bacteria and yeast,human or plant pathogens),the raw extract was able to potentiate the effect of beta-lactam antibiotics on methicillin-resistant Staphylococcus aureus(MRSA),reducing the minimal inhibitory concentrations(MICs) by a factor of 2-32-fold.No synergy was found between the extract and streptomycin.From the five isolated compounds only jaseosidin 5 showed a moderate antimicrobial activity. | Hanene Zater Joelle Huet Veronique Fontaine Samir Benayache Caroline Stevigny Pierre Duez Fadila Benayache | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 2 |
| 2 | Urinary cystatin C as a specific marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 3 | Urlnary cystatin C as aspe- cifle marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M et a/ | 2006 | Clin Chem Lab Meal2006，44，3： | 1 |
| 4 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 5 | Urinary cystatin C as aspecific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 6 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 7 | Urinary cystatin C as a specific marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Ghem Lab Med2006，44，3： | 1 |
| 8 | Urinary cystatin C as a specific marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 9 | Urinary cystatin C as a specific marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 10 | Urinary cystain C as a special marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，： | 1 |
| 11 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2010 | Clin Chem Lab Med2010，444，3： | 1 |
| 12 | Absence of circadian variations in urine cystatin C allows its use on urinary samples显示文摘 | CONTI M ZATER M LALLALI K | 2005 | Clin Chem2005，51，1： | 1 |
| 13 | Urinarycys- tatinCasaspecif - icmarkeroftubulardysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 14 | Urinary cystatin C as a specific marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 15 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2005 | Clin Chem Lab Med2005，44，3： | 1 |
| 16 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M et la | | 0，，03： | 1 |
| 17 | Urinary cystain C as a special marker of tubular dysfunction 显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chen Lab Med2006，44，： | 1 |
| 18 | Urinary Cystatin C as a specif marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2006 | Clin Chem Lab Med2006，44，3： | 1 |
| 19 | Urinary cystatin C as a specific marker of tubular dysfunction显示文摘 | Conti M Moutereau S Zater M | 2005 | Clin Chem Lab Med2005，44，3： | 1 |
| 20 | Absence of circadian variations in urine cystatin C allows its use on urinary samples显示文摘 | Conti M Zater M Lallali K | 2005 | Clin Chem2005，51，： | 1 |
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