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| 1 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 2 | Prey reduce risk-taking and abundance in the proximity of predators显示文摘 | Anders. R MФLLER Zbigniew KWIECINSKI Piotr TRYJANOWSKI | 2017 | Current Zoology2017，63，6： | 2 |
| 3 | Evolutionary algorithms,homomorphous mappings,and constrained parameter optimization显示文摘 | Slawomir K Zbigniew M | 1999 | Evolutionary Computation1999，7，1： | 1 |
| 4 | Theinfluence of polymer-surfactant aggregates on dragreduction显示文摘 | ZBIGNIEW M TADEUSZ M BARBARA G M | 2008 | Thin Solid Films2008，516，： | 1 |
| 5 | Microstruc- ture and properties of the new Pt-Rh based alloys for high-temperature applications 显示文摘 | Rdzawski Zbigniew M Stobrawa Jerzy P | 2004 | Journal of Materials Processing Technology2004，153，154： | 1 |
| 6 | Analysis of the carbonzation and formation of coal tar pitch mesophase under dynamic conditions显示文摘 | ANDRZEJ M STANISLAW B ZBIGNIEW R | 2003 | Carbon2003，41，： | 1 |
| 7 | Radar Interferometry with Public Domain Tools显示文摘 | Bert M Kampes Ramon F Hanssen Zbigniew Perski | 2003 | In Third International Workshop on ERS SAR Interferometry ''FRINGE0Y Frascati Italy2003，12，： | 1 |
| 8 | Wavelet transform based multifractal formalism in outlier detection and localization for finaocial time series显示文摘 | Zbigniew R Struzik Amo P J M | 2002 | Physica A2002，309，34： | 1 |
| 9 | Plasma fibronectin in pregnancy complicated by diabetes mellitus and preeclampsia 显示文摘 | Zbigniew C Tomasz M | 2008 | Ginekol Pol2008，79，7： | 1 |
| 10 | Microprocessor-Implemented SPWM for multiconverters with phase-shifted triangle carriers显示文摘 | BAKARI M ZBIGNIEW W BOON-TECK O | 1998 | IEEE Trans on Ind Appl1998，34，3： | 1 |
| 11 | The affact of Canopy Architecture on light interception and distribution in Sampion apple trees显示文摘 | Zbigniew B Augustyn M | 2009 | J Fruit Ornam Plant Res2009，17，2： | 1 |
| 12 | Parameter con- trol in evolutionary algorithms显示文摘 | Agoston E E Rober T H Zbigniew H M | 1999 | IEEE Trans on Evolu- tionary Computation1999，3，2： | 1 |
| 13 | Structural characterization of analgesic isothiazolopyridines of Mannich base type; X-ray analysis of 2- and 2--4, 6-dimethylisothiazolopyridin-3(2H)- ones 显示文摘 | Zbigniew K Wieslaw M | 2008 | J Mol Struct2008，888，： | 1 |
| 14 | Fatty acid ethyl esters decrease human hepatoblastoma cell proliferation and protein synthesis显示文摘 | ZBIGNIEW M SZCZEPIORKOWSKI G DICKERSIN R | | 0，，02： | 1 |
| 15 | hnportance of complete revascularization in patients with acute myocardial infarction treated with percutaneous coronary, intervention 显示文摘 | Zbigniew Kalarus M D Radoslaw Lenarczyk M D | 2007 | Am Heart2007，153，2： | 1 |
| 16 | Use of acid whey and mustard seed to replace nitrites during cooked sausage production 显示文摘 | Karolina M Wojciak Maigorzata Karwowska Zbigniew J Dolatowski | 2014 | Meat Science2014，，96： | 1 |
| 17 | Raman spectroscopy of high-pressure high-temperature polymorph of hexahydro-1,3,5-trinitro-1,3,5-triazine (ε-RDX)显示文摘 | Zbigniew A Dreger Yogendra M Gupta | | 0，，26： | 1 |
| 18 | Effects of uraemia and haemodialysis on neutrophil apoptosis and expression of apoptosis-related proteins显示文摘 | Ewa M Zbigniew B Zofia S | 2003 | Nephrol Dial Transplant2003，18，： | 1 |
| 19 | A hybrid particle swarm with a time-adaptive topology for constrained optimization 显示文摘 | Mohammad R B Xiang L Zbigniew M | 2014 | Suuarm and Evolution-ary Computation2014，18，10： | 1 |
| 20 | Structural Features of Economic Reform in Poland显示文摘 | Barbara M Roberts Jeffery I Round Zbigniew Zolkiewski | 1998 | Review of Development Economics1998，2，2： | 1 |
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