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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | 查看详情显示文摘 | D Kaszlikowski P Gnacinski M Zukowski W Mik laszewski and A Zeilinger | | 0，，21： | 1 |
| 3 | Second-order Optimality Conditions in Set Optimization显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 4 | Second-order optimality conditions in set optimization 显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 5 | Second-order Optimality Conditions in Set Optimization显示文摘 | Jahn J Khan AA Zeilinger P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 6 | Two adjacent protein binding motifs in the cbh2 (cellobiohydrolase II-encoding) promoter of the fungus Hypocrea jecorina (Trichoderma reesei) cooperate in the induction by cellulose显示文摘 | Zeilinger S Mach R L Kubicek C P | | 0，，51： | 1 |
| 7 | Second-Order Optimality Conditions in Set Optimization 显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | Journal of Optimizaaton Theory and Applications2005，125，2： | 1 |
| 8 | Different inducibility of espression of the two xylanase genes xyn1 and xyn2 in Trichoderma reesei显示文摘 | Zeilinger S Mach R L Schindler M Herzog P Kubicek C P | 1996 | J of Biological Chemistry1996，271，25： | 1 |
| 9 | Second-Order Optimality Conditions in Set Optimization 显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 10 | Second -order optimality conditions in set optimalization显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | J Optim Theory Appl2005，125，： | 1 |
| 11 | Demonstration of blind quantum computing显示文摘 | Barz S Kashefi E Broadbent A Fitzsimons J F Zeilinger A Wahher P | 2012 | Science2012，335，6066： | 1 |
| 12 | Two Adjacent Protein Binding Motifs in the CBH2 (CellobiohydrolaseII-encoding) Promoter of the Fungus Hypocrea jecorina (Trichoderma reesei) Cooperate in the Induction by Cellulose显示文摘 | Robert L Mach Christian P Kubicek | 1998 | The Journal of Biological Chemistry1998，273，34： | 1 |
| 13 | Cell detachment during sinusoidal reperfusion after liver preservation: an in vitro model显示文摘 | Gerlach JC Hentschel F Spatkowski G Zeilinger K Smith MD Neuhaus P | 1997 | Transplanlation1997，64，6： | 1 |
| 14 | Second-order opti- mality conditions in set optimization显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | J Optim Theory Appl2005，125，： | 1 |
| 15 | Second-order optimality conditions in set optimization显示文摘 | Jahn J Khan A A Zeilinger P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 16 | Second-order optimality conditions in set optimization显示文摘 | JAHN J KHAN A A ZEILINGER P | 2005 | J Optim Theory Appl2005，125，： | 1 |
| 17 | Regulation of-xylosidase formation by xylose in Trichoderma reesei 显示文摘 | Kristufek D Zeilinger S Kubicek C P | 1995 | Applied Microbiology and Biotechnology1995，42，5： | 1 |
| 18 | Second-order optimality conditions in set optimalization显示文摘 | JAHN J KHAN A A ZEILINGER P | 2005 | J Optim Theory Appl2005，125，： | 1 |
| 19 | Second-order optimality condifions in set optimization显示文摘 | Jahn J Khan A A Zeilinge P | 2005 | Journal of Optimization Theory and Applications2005，125，2： | 1 |
| 20 | Study of signal transduction factors involved in mycoparasitic response of Trichoderma atroviride显示文摘Numerous Trichoderma spp. are mycoparasites and commercially applied as biological control agents against a large number of plant pathogenic fungi. The mycoparasitic interaction is host-specific and several research strategies have been applied to identify the main genes and compounds involved in the antagonist-plant-pathogen three-way interaction. During mycoparasitism, signals from the host fungus are recognised by Trichoderma, stimulating antifungal activities that are accompanied by morphological changes and the secretion of hydrolytic enzymes and antibiotics. Interestingly some morphological changes appeared highly conserved in the strategy of pathogenicity within the fungal world, i.e. the formation of appressoria as well as the secretion of hydrolytic enzymes seem to be general mechanisms of attack both for plant pathogens and mycoparasitic antagonists. This knowledge is being used to identify receptors and key components of signalling pathways involved in fungus-fungus interaction. For this purpose we have cloned the first genes (tmk1, tga1, tga3) from T. atroviride showing a high similarity to MAP kinase and G protein subunits (see abstract by Zeilinger et al.), which have been found to have an important role in pathogenicity by Magnaporthe grisea. To identify the function and involvement of these factors in mycoparasitism by T. atroviride, tmk1, tga1, tga3 disruptant strains were produced. The knock-out mutants were tested by in vivo biocontrol assays for their ability to inhibit soil and foliar plant pathogens such as Rhizoctonia solani, Pythium ultimum and Botrytis cinerea . Disruption of these genes corresponded to a complete loss of biocontrol ability, suggesting a significant role in mycoparasitism. In particular, it has been suggested that tga3 regulates the expression of chitinase-encoding genes, the secretion of the corresponding enzymes and the process of conidiation. Comparative proteome analysis of wild type and disruptants supported this hypothesis, and indicated many changes in the protein profiles of T. atroviride in different interaction conditions with plants and pathogenic hosts. | Scala V Zeilinger S Ambrosino P Brunner K Reithner B Mach R L Woo S L Cristilli M Scala F Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 1 |
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