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| 1 | TaCML36, a wheat calmodulin-like protein,positively participates in an immune response to Rhizoctonia cerealis显示文摘Sharp eyespot,mainly caused by the soil-borne fungus Rhizoctonia cerealis,affects wheat(Triticum aestivum L.)production worldwide.In this study,we isolated TaCML36 gene encoding a wheat calmodulin-like protein,and studied its defense role in protection against R.cerealis.Transcription of TaCML36 was significantly elevated by both R.cerealis infection and exogenous ethylene treatment.Transcription was higher in resistant wheat lines than in susceptible ones.There were copies of TaCML36 on chromosomes 5A,5B,and 5D.The TaCML36 protein is composed of 183 amino acids and contains two calcium-binding EFhand domains.Subcellular localization assays in wheat indicated that TaCML36 localizes in both the cytoplasm and nucleus.Virus-induced gene silencing and disease assessment indicated that compared to the controls,TaCML36-silenced wheat plants displayed significantly reduced resistance to R.cerealis and had greater fungal biomass,suggesting that knockdown of TaCML36 impaired host resistance.Knockdown of TaCML36 also significantly repressed expression of pathogenesis-related genes such as Chitinase 1,PDF35,and PR17C,the ethylene response factor-encoding gene TaPIE1,and ethylene biosynthesis gene ACO2.Collectively,our results suggest that TaCML36 positively participates in the innate immune response to R.cerealis infection by modulating expression of defense-associated genes possibly in the ethylene signaling pathway. | Lin Lu Wei Rong Ronghua Zhou Naxin Huo Zengyan Zhang | 2019 | The Crop Journal2019,7,5: | 3 |
| 2 | The GATA transcription factor TaGATA1 recruits demethylase TaELF6-A1 and enhances seed dormancy in wheat by directly regulating TaABI5显示文摘Seed dormancy is an important agronomic trait in crops, and plants with low dormancy are prone to preharvest sprouting(PHS) under high-temperature and humid conditions. In this study,we report that the GATA transcription factor TaGATA1 is a positive regulator of seed dormancy by regulating TaABI5 expression in wheat.Our results demonstrate that TaGATA1 overexpression significantly enhances seed dormancy and increases resistance to PHS in wheat. Gene expression patterns, abscisic acid(ABA) response assay, and transcriptome analysis all indicate that TaGATA1 functions through the ABA signaling pathway. The transcript abundance of TaABI5, an essential regulator in the ABA signaling pathway,is significantly elevated in plants overexpressing TaGATA1. Chromatin immunoprecipitation assay(ChIP) and transient expression analysis showed that TaGATA1 binds to the GATA motifs at the promoter of TaABI5 and induces its expression.We also demonstrate that TaGATA1 physically interacts with the putative demethylase TaELF6-A1, the wheat orthologue of Arabidopsis ELF6.ChIP–qPCR analysis showed that H3K27me3 levels significantly decline at the TaABI5 promoter in the TaGATA1-overexpression wheat line and that transient expression of TaELF6-A1 reduces methylation levels at the TaABI5 promoter, increasing TaABI5 expression. These findings reveal a new transcription module, including TaGATA1–TaELF6-A1–TaABI5, which contributes to seed dormancy through the ABA signaling pathway and epigenetic reprogramming at the target site. TaGATA1 could be a candidate gene for improving PHS resistance. | Xuening Wei Yuyan Li Xiuliang Zhu Xin Liu Xingguo Ye Miaoping Zhou Zengyan Zhang | 2023 | Journal of Integrative Plant Biology2023,65,5: | 1 |
| 3 | Solvents adjusted pure phase CoCO_(3) as anodes for high cycle stability显示文摘CoCO_(3) with high theoretical capacity has been considered as a candidate anode for the next generation of lithium-ion batteries(LIBs).However,the electrochemical performance of CoCO_(3) itself,especially the cyclic stability at high current density,hinders its application.Herein,pure phase CoCO_(3) particles with different particle and pore sizes were prepared by adjusting the solvents(diethylene glycol,ethylene glycol,and deionized water).Among them,CoCO_(3) synthesized with diethylene glycol(DG-CC)as the solvent shows the best electrochemical performance owing to the smaller particle size and abundant mesoporous structure to maintain robust structural stability.A high specific capacity of 690.7 mAh/g after 1000 cycles was achieved,and an excellent capacity retention was presented.The capacity was contributed by diverse electrochemical reactions and the impedance of DG-CC under different cycles was further compared.Those results provide an important reference for the structural design and stable cycle performance of pure CoCO_(3). | Liming LIU Xiaoxiao HUANG Zengyan WEI Xiaoming DUAN Bo ZHONG Long XIA Tao ZHANG Huatao WANG Dechang JIA Yu ZHOU Rui ZHANG | 2021 | Journal of Advanced Ceramics2021,10,3: | 0 |
| 4 | Review of water pollution control in China显示文摘Water resource shortage and pollution has seriously threatened the survival and development of developing countries.Because of China’s specific economical and social circumstances,complete adoption of developed countries’experience is unrealistic.At present,China needs to develop strategies and technologies in source water pollution control and municipal environmental remediation that embrace the country’s specific need to battle the water resource problem.Among them,efficient source water pretreatment is a critical step to ensure a safe municipal water supply.Unlike developed countries,it is not yet feasible in China to treat water supplied to the household and have it meet the standard of direct drinking;therefore,it is more appropriate to refer to it as service water.As a beneficial supplement,an additional community drinking water network and household drinking water apparatus can be considered. | Tingyao GAO Hongbin CHEN Siqing XIA Zengyan ZHOU | 2008 | Frontiers of Environmental Science & Engineering2008,2,2: | 0 |