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您的检索式：作者名="Zhanbiao Yu"
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| 1 | miR-100-5p in human umbilical cord mesenchymal stem cell-derived exosomes mediates eosinophilic inflammation to alleviate atherosclerosis via the FZD5/Wnt/β-catenin pathway显示文摘Exosomes derived from human umbilical cord mesenchymal stem cells(hUCMSC-Ex)play important roles in immune and inflammation diseases.However,the role of hUCMSC-Ex in atherosclerosis has not been elucidated.In this study,the isolated exosomes were identified by transmission electron microscopy and nanoparticle tracking analysis.Exosome marker protein levels were increased in the hUCMSC-Ex compared with those in hUCMSC suspension,indicating that exosomes were successfully isolated from hUCMSCs.Furthermore,eosinophils were treated with oxidized low-density lipoprotein(ox-LDL)to construct inflammation model and then incubated with hUCMSC-Ex derived from hUCMSCs which were transfected with miR-100-5p mimic or miR-100-5p inhibitor.We found that hUCMSC-Ex increased miR-100-5p expression,inhibited cell migration,promoted cell apoptosis,and reduced inflammatory cytokine levels in ox-LDL-treated eosinophils,and miR-100-5p overexpression in hUCMSCs enhanced these effects,while miR-100-5p inhibition reversed these effects.Moreover,frizzled 5(FZD5)was a target gene of miR-100-5p.FZD5 overexpression reversed the inhibitory effects of hUCMSC-Ex-miR-100-5p on cell progression and inflammation in eosinophils.Additionally,hUCMSC-Ex-miR-100-5p decreased the expression of cyclin D1 andβ-catenin proteins.Wnt/β-catenin pathway activator BML-284 effectively reversed the effects of hUCMSC-Ex-miR-100-5p on cell progression and inflammation in eosinophils.ApoE−/−mice were fed with high-fat diet to construct an atherosclerosis mice model,and hUCMSC-Ex was injected into mice.hUCMSC-Ex reduced atherosclerotic plaque area and inflammation response in atherosclerosis mice.This study demonstrates that hUCMSC-Ex-miR-100-5p inhibits cell progression and inflammatory response in eosinophils via the FZD5/Wnt/β-catenin pathway,thereby alleviating atherosclerosis progression. | Heng Gao Zhanbiao Yu Yuanyuan Li Xue Wang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，9： | 3 |
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