| 1 | Dietary host-associated Bacillus subtilis supplementation improves intestinal microbiota,health and disease resistance in Chinese perch(Siniperca chuatsi)显示文摘The purpose of this study was to evaluate the potential of a host-associated Bacillus subtilis 1-C-7 as a probiotic for Chinese perch(Siniperca chuatsi).Four test diets were formulated to contain graded levels of B.subtilis 1-C-7 at 0(CY),0.85×10^(8)(Y1),0.95×10^(9)(Y2)and 0.91×10^(10)(Y3)CFU/kg diet.The test fish with initial weight 30.0±1.2 g were fed the 4 test diets with 3 replicates in an indoor water-flow aquaculture system with 12 net cages(40 fish/cage)for 10 wk.At the conclusion of the feeding trial,the probiotic effects of B.subtilis on Chinese perch were analyzed based on growth performance,serum biochemical indices,histologic morphology of liver and gut,gut microbiota and the resistance to Aero-monas hydrophila.The results showed that the percentage of weight gain had no significant change in the Y1 and Y2 groups(P>0.05)but decreased in the Y3 group compared to that in the CY group(P<0.05).The fish in the Y3 group displayed the highest activity of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)among these 4 groups(P<0.05).The fish in the CY group had the highest value of malondialdehyde in the liver(P<0.05)and showed severe nuclear migration and vacuolization of hepatocytes.The morphology indicated that all test fish had poor intestinal health.However,the fish in the Y1 group had a relatively normal intestinal histologic structure.The mid gut microbial diversity analysis showed that dietary B.subtilis supplementation increased the abundance of probiotics such as Tenericutes and Bacteroides,whereas it reduced the abundance of pernicious bacteria such as Proteobacteria,Actinobacteria,Thermophilia and Spirochaetes.The challenge test showed that dietary B.subtilis supplementation increased the resistance to A.hydrophila in Chinese perch.In conclusion,dietary supplementation of 0.85×10^(8)CFU/kg B.subtilis 1-C-7 could improve the intestinal microbiota,intestinal health and disease resistance in Chinese perch,but more or excessive supple-mentation could reduce growth performance and have negative effects on health. | Zhehui Ji Chuanzhong Zhu Xinyang Zhu Sainan Ban Lijuan Yu Juan Tian Lixue Dong Hua Wen Xing Lu Ming Jiang | 2023 | Animal Nutrition2023，，2： | 0 |
| 2 | The probiotic effects of host-associated Bacillus velezensis in diets for hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂)显示文摘This study was to evaluate the potential of a host-associated Bacillus velezensis as a probiotic for hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂).Diets(B0 to B5)containing 0,0.90×10^(8),0.80×109,0.85×10^(10),0.90×10^(11),0.83×10^(12)CFU/kg B.velezensis YFI-E109 were fed to the fish with initial weight(3.07±0.08 g)in a recirculating aquaculture system for six weeks with three replicates,respectively.Probiotic effects were analyzed based on growth,body composition,liver and gut morphology,gut microbiome,and liver metabolome.Analysis of the bacterial genome has shown that the most abundant genes in B.velezensis YFI-E109 were distributed in carbohydrate and amino acid metabolism.Fish in groups B3 and B4 had better growth performance,and higher intestinal amylase(AMS)and lipase(LPS)activities compared with other groups(P<0.05).Fish in groups B0 and B5 showed significant liver damage,while this status improved in group B3.The liver malondialdehyde(MDA)content in group B3 was lower than that in other groups(P<0.05).The abundance of Mycoplasma,Ralstonia and Acinetobacter was significantly reduced in B3 and B5 compared to B0.The amino acid and carbohydrate metabolism pathways were enriched in group B3 compared with group B0.In conclusion,dietary B.velezensis YFI-E109 supplementation has the potential to improve growth,liver metabolism,and liver and gut health,and reshape the gut microbiome of hybrid yellow catfish.Excessive B.velezensis YFI-E109 reduced the prebiotic effects.The recommended dietary supplementation of B.velezensis YFI-E109 is 0.31×10^(10) to 0.77×10^(11)CFU/kg for hybrid yellow catfish according to the quadratic regression method by plotting specific growth rate(SGR),feed conversion ratio(FCR),MDA and activities of AMS against dietary B.velezensis YFI-E109 levels. | Zhehui Ji Xing Lu Mingyang Xue Yuding Fan Juan Tian Lixue Dong Chuanzhong Zhu Hua Wen Ming Jiang | 2023 | Animal Nutrition2023，，4： | 0 |
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