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| 1 | Selective blockade of NF-kappa B by novel mutated I kappa B alpha suppresses CD3/CD28-induced activation of memory CD4(+) T cells in asthma显示文摘Background:Nuclear factor kappa B(NF-kappa B) overactivation plays a crucial role in T-helper 2 (Th2)-biased allergic airway inflammation by increased activation and decreased apoptosis of CD4(+) T cells. We have shown that targeted NF-kappa B suppression in dendritic cells by adenoviral gene transfer of a novel mutated inhibitor of NF-kappa B(I kappa B alpha) (AdI kappa B alpha M) contributes to T-cell tolerance, but the immunosuppressive action of AdI kappa B alpha M on memory(CD45RO(+)) CD4(+) T cells remains enigmatic. Methods:CD45RO(+) T cells from Dermatophagoides farinaei-sensitized asthmatic patients, untransfected or transfected with AdI kappa B alpha M or AdLacZ(beta-galactosidase) for 24 h, were stimulated with anti-CD3(1.0 mu g/ml) plus anti-CD28(0.5 mu g/ml) monoclonal antibody for an additional 24 h. I kappa B alpha M transgene expression and NF-kappa B activation were detected by polymerase chain reaction(PCR), reverse transcription-PCR(RT-PCR), Western blot analysis, and electrophoretic mobility shift assay. Phenotype and apoptosis were measured by flow cytometry, annexin V binding, and terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling analyses. Cytokine production and cell proliferation were determined using enzyme-linked immunosorbent assay andH-3 thymidine incorporation. Results:A unique 801-bp I kappa B alpha M cDNA and a dose-dependent increase in I kappa B alpha M transgene expression were observed in AdI kappa B alpha M-transfected CD45RO(+) T cells. Significantly, AdI kappa B alpha M inhibited CD3/CD28-mediated NF-kappa B activation in CD45RO(+) T cells, leading to evident apoptosis, reduction of eotaxin, RANTES, Th1 interferon (IFN)-gamma and interleukin (IL)-2, and Th2 (IL-4, IL-5, and IL-13 despite a slight decrease in IL-10) cytokines and secondary proliferative response. AdI kappa B alpha M also upregulated cytotoxic T lymphocyte-associated antigen 4(CTLA-4) and downregulated CD69 besides no change in CD28. Conclusion:I kappa B alpha M might be beneficial to augment memory CD4(+) T-cell tolerance through modulating B7-CD28/CTLA-4 co-stimulatory pathways and NF-kappa B-dependent cytokine profiles in allergic inflammatory diseases including asthma. | Zhou,L. F. Zhang,M. S. Hu,A. H. Zhu Z. Yin,K. S. | 2008 | 南京医科大学学报(自然科学版)2008，28，6： | 40 |
| 2 | Common Genetic Variants in Pre-MicroRNAs Were Associated With Increased Risk of Breast Cancer in Chinese Women显示文摘 | Hu, Z. B. Liang, J. Wang, Z. W. Tian, T. Zhou, X. Y. Chen, J. P. Miao, R. F. Wang, Y. Wang, X. R. Shen, H. B. | 2009 | 南京医科大学学报(自然科学版)2009，29，4： | 29 |
| 3 | Development and application of functional gene arrays for microbial community analysis显示文摘功能的基因标记能关于功能的基因差异和微生物引起的社区的潜在的活动提供重要信息。尽管 microarray 技术成功地被使用了为纯文化学习基因表示,简单、人工的微生物引起的社区,改编如此的一种技术分析复杂微生物引起的社区仍然以设计,样品准备,和数据分析提出很多挑战。这个工作集中于功能的基因数组(FGA ) 的开发和应用程序为微生物引起的社区研究指向关键功能的基因标记。一些与 FGA 有关明确地给问题调音,例如 oligonucleotide 探查设计, nucleic 酸抽取和纯化,数据分析,特性,敏感,和量的能力详细被讨论。最近的研究证明了 FGA 能从许多自然环境关于微生物引起的社区提供特定、敏感、潜在地量的信息并且控制生态系统。这种技术被期望革命化微生物引起的社区的分析,并且连接微生物引起的结构到生态系统工作。 | Z. L. HE J. D. VAN NOSTRAND L.Y. WU J. Z. ZHOU | 2008 | 中国有色金属学会会刊:英文版2008，18，6： | 21 |
| 4 | Contamination by Organochlorine Pesticides in the Estuaries of Southeast China显示文摘Persistent organic pollutants(POPs) such as organochlorine pesticides are of global concern due to their widespread occurrence and persistence. This paper reports recent research studying the distribution and fate of organochlorine pesticides in the Jiulong River, the Minjiang River and the Pearl River estuaries in Southeast China. Eighteen organochlorine pesticides were extracted from water, pore water and sediment samples, followed by analysis by GC ECD. The results showed that the contamination levels were similar in these three estuaries. The levels of the total organochlorine pesticides in porewater were significantly higher than those in surface water, due to the high affinity of these hydrophobic compounds for sediment phase. Among the hexachlorocyclohexane(HCH) compounds, β HCH was found to be the most important isomer. The analysis of 1,1,1 trichloro 2,2 bis chlorophenyl ethane(DDT) and its metabolites showed that 1,1 dichloro 2[ o chlorophenyl] 2[ p chlorophenyl] ethylene(DDE) was dominant in the group. | Z. L. Zhang 1,2 , H. S. Hong 2, J. L. Zhou 3,** , M. H. Dai 2, K. Maskaouib 2 and W. Q. Chen 2 1. Department of Environmental Science and Engineering, Tsinghua POPs Research Centre, Tsinghua University, Beijing 100084, China 2. Key Laborator | 2002 | Chemical Research in Chinese Universities2002，18，2： | 13 |
| 5 | Voluntary exercise-induced neurogenesis in the postischemic dentate gyrus is associated with spatial memory recovery from stroke显示文摘 | Luo, C. X. Jiang, J. Zhou, Q. G. Zhu, X. J. Wang, W. Zhang, Z. J. Han, X. Zhu, D. Y. | 2007 | 南京医科大学学报(自然科学版)2007，27，10： | 13 |
| 6 | Polymorphisms of MTHFD,plasma homocysteine levels, and risk of gastric cancer in ahigh-risk Chinese population显示文摘Purpose:Accumulative evidence suggests that folate has a protective effect on gastric cancer. The methylenetetrahyd-rofolate dlehydrogenase(MTHFD) plays an important role in folate and homocysteine metabolisms, and polymorphisms of MTHFD may result in disturbance of the folate-mediated homocysteine pathway. The aim of this study is to test the hypothesis that genetic variants of MTHFD and plasma homocysteine levels are associated with risk of gastric cancer and modulated by genotypes of methylenetetrahydrofolate reductase(MTHFR). Experimental Design: We genotyped G1958A and T401C in MTHFD and C677T in MTHFR and detected total plasma homocysteine(tHcy) levels in a case-control study of 589 gastric cancer cases and 635 cancer-free controls in a high-risk Chinese population. Results:The variant genotypes of MTHFD 1958AA and 401CC were associated with a significantly increased risk of gastric canceradjusted odds ratio(OR), 2.05; 95% confidence interval(95% CI),1.34-3.13 for 1958AA; adjusted OR,1.43; 95% CI,1.14-1.80 for 401CC compared with 1958GG/GA and 401TT/TC genotypes, respectively. Both of the effects were more evident in the subjects carrying MTHFR 677CT/TT genotypes. The average tHcy level was significantly higher in gastric cancer cases than in controls(P < 0.01), and the upper quartile of tHcy (> 13.6 mu mol/L) was associated with an 82% significantly increased risk of gastric cancer, compared with the lowest quartile of tHcy(<= 8.0 pmol/L;adjusted OR,1.82; 95% CI,1.20-2.75). Conclusions:The strong associations between MTHFD variants and the plasma tHcy levels and gastric cancer risk suggest, for the first time, a possible gene-environment interaction between genetic variants of folate-metabolizing genes and high tHcy levels in gastric carcinogenesis. | Wang, L. N Ke, Q Chen, W. S Wang, J. M Tan, Y. F Zhou, Y Hua, Z. L Ding, W. L Niu, J. Y Shen, J Zhang, Z. F Wang, X. R Xu, Y. H Shen, H. B Yi Xing | 2007 | 南京医科大学学报(自然科学版)2007，27，7： | 10 |
| 7 | Identification of JWA as a novel functional gene responsive to environmental oxidative stress induced by benzo a pyrene and hydrogen peroxide显示文摘 | Chen, R. Qiu, W. Liu, Z. L. Cao, X. J. Zhu, T. Li, A. P. Wei, Q. Y. Zhou, J. W. | 2007 | 南京医科大学学报(自然科学版)2007，27，10： | 9 |
| 8 | Proteomic-based identification of maternal proteins in mature mouse oocytes显示文摘 | Zhang, P. Ni, X.J. Guo, Y. Guo, X. J. Wang, Y. F. Zhou, Z. M. Huo, R. Sha, J. H. | 2009 | 南京医科大学学报(自然科学版)2009，29，11： | 8 |
| 9 | Variant alleles of TGFB1 and TGFBR2 are associated with a decreased risk of gastric cancer in a Chinese population显示文摘 | Jin, G. F. Wang, L. N. Chen, W. S. Hu, Z. B. Zhou, Y. Tan, Y. F. Wang, J. M. Hua, Z. L. Ding, W. L. Shen, J. Zhang, Z. F. Wang, X. R. Xul, Y. C. Shen, H. B. | 2007 | 南京医科大学学报(自然科学版)2007，27，5： | 7 |
| 10 | Variant genotypes and haplotypes of the epidermal growth factor gene promoter are associated with a decreased risk of gastric cancer in a high-risk Chinese population显示文摘 | Jin, G. F. Miao, R. F. Deng, Y. M. Hu, Z. B. Zhou, Y. Tan, Y. F. Wang, J. M. Hua, Z. L. Ding, W. L. Wang, L. Chen, W. S. Shen, J. Wang, X. R. Xu, Y. C. Shen, H. B. | 2007 | 南京医科大学学报(自然科学版)2007，27，10： | 7 |
| 11 | A sperm GPI-anchored protein elicits sperm-cumulus cross-talk leading to the acrosome reaction显示文摘 | Yin, L. Chung, C. M. Huo, R. Liu, H. Zhou, C. Xu, W. Zhu, H. Zhang, J. Shi, Q. Wong, H. Y.C. Chen, J. Lu, Y. Bi, Y. Zhao, C. Du, Y. Ma, M. Cai, Y. Chen, W. Y. Fok, K. L. Tsang, L. L. Li, K. Ni, Y. Chung, Y. W. Zhou, Z. Sha, J. Chan, H. C. | 2009 | 南京医科大学学报(自然科学版)2009，29，4： | 5 |
| 12 | Effects of Dietary Supplementation with Cysteamine on Performance, Carcass Characteristics, Meat Quality and Antioxidant Status in Finishing Pigs显示文摘 | X. Z. Lv Y. Wang G. L. Liu Z. S. Wang A. G. Zhou | 2011 | Journal of Agricultural Science and Technology2011，5，1： | 5 |
| 13 | DHEA-neuroprotection and -neurotoxicity after transient cerebral ischemia in rats显示文摘 | Li, Z. Cui, S. Z. Zhang, Z. Zhou, R. Ge, Y. B. Sokabe, M. Chen, L. | 2009 | 南京医科大学学报(自然科学版)2009，29，4： | 4 |
| 14 | Complete closure of large gastric defects after endoscopic full-thickness resection, using endoloop and metallic clip interrupted suture显示文摘 | Q. Shi T. Chen Y.-S. Zhong P.-H. Zhou Z. Ren M.-D. Xu L.-Q. Yao | 2013 | Endoscopy2013，，05： | 3 |
| 15 | Ultrastructural analysis of neuronal synapses using state-of-the-art nano-imaging techniques显示文摘Neuronal synapses are functional nodes in neural circuits.Their organization and activity define an individual's level of intelligence,emotional state and mental health.Changes in the structure and efficacy of synapses are the biological basis of learning and memory.However,investigation of the molecular architecture of synapses has been impeded by the lack of efficient techniques with sufficient resolution.Recent developments in state-of-the-art nano-imaging techniques have opened up a new window for dissecting the molecular organization of neuronal synapses with unprecedented resolution.Here,we review recent technological advances in nano-imaging techniques as well as their applications to the study of synapses,emphasizing super-resolution light microscopy and 3-dimensional electron tomography. | Changlu Tao Chenglong Xia Xiaobing Chen Z. Hong Zhou Guoqiang Bi | 2012 | Neuroscience Bulletin2012，28，4： | 3 |
| 16 | Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis显示文摘 | L. Ouyang Z. Shi S. Zhao F.‐T. Wang T.‐T. Zhou B. Liu J.‐K. Bao | 2012 | Cell Prolif2012，，6： | 3 |
| 17 | MORPHOLOGY OF AMORPHOUS SILICA DURING ALUMINUM REACTIVE PENETRATION显示文摘The reduction of the volumes of the silica glass during aluminum reactive penetration was observed experimentally. The liquid aluminum infiltrates easily into the cavities while the released silicon solves in the liquid aluminum, and Al/A12 O3 composites could be obtained finally. The experimental result has shown that Al/A12 O3 composites could transform into A12O3 grains by oxygen in the cavities of Al/A12O3 composites during longer penetration treatment. The formation of alumina is a sintering process, in which liquid aluminum, particulate alumina and oxygen play an important role. Tin nsformed process has shown that there is a relationship of σAl2O3 | Zhou, Z. Gan, Q.S. Ding, P.D. | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，4： | 3 |
| 18 | Comparative proteome profile of immature rat ovary during primordial follicle assembly and development显示文摘 | Wang, N. L. Zhang, P. Guo, X. J. Xie, J. Huo, R. Wang, F. Q. Chen, L. Shen, J. Zhou, Z. M. Shi, Q. H. Zhao, B. G. Sha, J. H. | 2009 | 南京医科大学学报(自然科学版)2009，29，11： | 3 |
| 19 | Degradation of aniline by newly isolated, extremely aniline-tolerant Delftia sp. AN3显示文摘 | Z. Liu H. Yang Z. Huang P. Zhou S.-J. Liu | 2002 | Applied Microbiology and Biotechnology2002，，5： | 2 |
| 20 | Ageing of Stored Rice: Changes in Chemical and Physical Attributes显示文摘 | Z. Zhou K. Robards S. Helliwell C. Blanchard | 2002 | Journal of Cereal Science2002，，1： | 2 |
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