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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Clinical and dosimetric predictors of radiation-induced esophageal toxicity 显示文摘 | Maguire PD Sibley GS Zhou SM | 1999 | Int J Radiat Oncol Biol Phys1999，45，1： | 1 |
| 3 | Clinical and dosimetric predictors radiation-induced esophageal toxicity显示文摘 | Maguire PD Sibley GS Zhou SM | 1999 | Int J Radiat Oncol Biol Phys1999，45，： | 1 |
| 4 | Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass显示文摘 | Xu ZZ Zhou GS | 2008 | J Exp Bot2008，59，12： | 1 |
| 5 | Protective effects of demethylbellidifolin on myocardial ischemia-reperfusion injury in rats显示文摘 | Tan GS Zhou ZH | 2002 | Planta Med2002，68，8： | 1 |
| 6 | Regional volumetric coronary blood flow measurement by digital angiography : In vivo validation 显示文摘 | Molloi S Zhou Y Kassab GS | 2004 | Acad Radiol2004，11，7： | 1 |
| 7 | JAK-STAT signaling path way in pul monary arterial smooth muscle cells is activated by hypoxia显示文摘 | Wang GS Qian GS Zhou DS | 2005 | Cell Biol Int2005，29，7： | 1 |
| 8 | Extraction of sesamin from sesame oil using macroporous resin显示文摘 | Zhou JC Feng DW Zheng GS | | 0，，： | 1 |
| 9 | Sulphur byproduct: The relationship volatile sulphur compounds and dental plaqueinduced gingivitis 显示文摘 | Zhou H Me Combs GS Darby ML | 2004 | J Contemp Dent Pract2004，5，2： | 1 |
| 10 | Detection of the airway obstruction stage in asthma using impulse oscillometry system显示文摘 | Qi GS Zhou ZC Gu WC | 2013 | J Asthma2013，50，1： | 1 |
| 11 | The role of mitogen-activated protein ki- nase phosphatase-1 in oxidative damage-induced cell death 显示文摘 | Zhou JY Liu Y Wu GS | 2006 | Cancer Res2006，66，48： | 1 |
| 12 | Comparative characteriza- tion of total flavonol glycosides and terpene lactones at different ages, from different cultivation sources and genders of Ginkgo biloba leaves 显示文摘 | Yao X Shang EX Zhou GS | 2012 | Int J Mol Sci2012，13，10： | 1 |
| 13 | Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass显示文摘 | XU ZZ ZHOU GS | 2008 | Journal of Experimental Botany2008，59，12： | 1 |
| 14 | Isoliquiritigenin inhibits proliferation and induces apoptosis of U87 human glioma cells in -itro 显示文摘 | Zhou GS Song LJ Yang B | 2013 | Mol Med Report2013，7，2： | 1 |
| 15 | The embryology of Xanthoceras and its phylogenetic implications显示文摘 | Zhou QY Liu GS 2012 | 2012 | Plant Systematics and Evolution2012，298，： | 1 |
| 16 | A UPLC-MS/MS method for qualification of quercetin-3 -O-β-D-glucopyranosyl-(4→1)-α-L- rhamnoside in rat plasma and application to pharmacokinetic studies 显示文摘 | Yao X Zhou GS Tang YP | 2013 | Molecules2013，18，3： | 1 |
| 17 | JAK-STAT signaling pathway in pulmonary arterial smooth muscle cells is activated by hypoxia显示文摘 | Qian GS Zhou DS | 2005 | Cell Biol Int2005，29，7： | 1 |
| 18 | Vitrification of farmed blue fox oocytes in ethylene glycol and DMSO-based solutions using open- pulled straw (OPS) 显示文摘 | Zhou GB Ma CB Liu GS | 2009 | Cryo Letters2009，30，2： | 1 |
| 19 | Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass 显示文摘 | Xu ZZ Zhou GS 2008 | 2008 | Journal of Experimental Botany2008，59，12： | 1 |
| 20 | Clinical and dosimetric predictor of radiation induced esophageal toxicity显示文摘 | Maguire PD Sibley GS Zhou SM | 1999 | Int J Radiat Oncol Phys1999，45，1： | 1 |
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