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| 1 | Fresh Amniotic Membrane Transplantation for Conjunctival Surface Reconstruction显示文摘Purpose:To determine whether fresh human amniotic membrane can be used to reconstruct the conjunctiveal defect creates during symblepharon lysis.Methods:Forth-two eyes of 39 consecutive patients with eye burns and Stevens-Johnson syndrome were randomized to accept fresh or preserved human amniotic membrane transplantation(AMT) during the period of severe scarring.Impression cytology was performed in 12 eyes with normal tear secretion which received fresh AMT.Results:During a mean follow-up of 11 months (range,6 to 18 months),thirty-five patients (37 eyes)showed successful ocular surface reconstruction and resolution of motility restriction while four patients (2 eyes with fresh AMT,3 eye with preserved AMT)with minimal recurrence of symblepharon.There was no significant difference statistically between two groups(Chi-square test).Amniotic epithelial cells can survive about three months after being transplanted onto ocular surfaces with normal tear secretion.Conclusion:Both fresh and preserved human amniotic membrane can be considered an ideal alternative substrate for conjunctival surface reconstruction during removal of severe symblepharon. | ZhouSY ChenJQ | 1999 | 眼科学报1999，15，3： | 11 |
| 2 | Preparation oftitania micro filtration membranes supported on porous Ti-A1 alloys显示文摘 | ZhouSY FanY Q He Y H etal | 2008 | J Membr Sci2008，325，2： | 1 |
| 3 | Variable depth thermal lesions in the rabbit cornea created using a novel thulium fiber laser with tunable wavelength显示文摘 | ZHOUSY NOGUERA G CASTRO J | 2006 | Invest Ophthalmol Vis Sci2006，47，： | 1 |
| 4 | Active balancing and vibration control of rotating machinery: A survey显示文摘 | ZhouSY Shi J J | | 0，，： | 1 |
| 5 | 显示文摘 | XIAOXiao-hua(肖小华) ZHOUSi(周思) LIGong-ke(李攻科) | 2012 | 食品安全质量检测学报2012，5，： | 1 |
| 6 | A Study on the Preservation of Fresh Amniotic Membrane显示文摘Objective: To establish the standard preservation methods of fresh amniotic membranefor clinical use.Methods: Human placentas were collected aseptically from selective caesarean sectionsin normal women in time. Amniotic or placental membrane were peeled and preserved inN.S, P.B. SorDMEM at4°C or cultured in DMEM at 37°C, 5% CO2. Trypan-bluestaining, light and electronic microscopy were observed every six hours after preservation.Results: Seventy percent of amniotic epithelial cells survived after preservation in N. Sfor 6 hours, PBS 12 hours, DMEM 24 hours and 1 week in tissue culture. The amountof living epithelial cells maintained in placental membrane preservation was less thanthat in amniotic membrane preservation at the same time (t-test, P < 0. 01) . Nocollagen degeneration was found during preservation.Conclusion: Preservative solution and time will affect the maintenance time of freshamniotic membrane greatly. Fresh amniotic membrane should be preserved within 6hours in N.S, 12 hours in P.B.S, 24 hours in DMEM at 4 °C and 1 week in tissteculture for clinical use. | XuLY ZhouSY 等 | 2001 | 眼科学报2001，17，3： | 0 |
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