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| 1 | Mechanism of Incompatible Herb Pairs, Panax ginseng and Veratrum nigrum L,: Material Basis and Metabolic Profiles of Ginsenosides in Rat Intestinal Bacteria显示文摘在繁体中文药理论,人参属人参和 Veratrum nigrum L。是重要不兼容的植物对。在老鼠的新陈代谢上的主要部件和影响的内容变化上的研究肠的细菌是有用的理解这的不兼容的机制植物对。在这研究,人参皂甙和他们的 metaboltic 的内容变化在 P 的摘录介绍。P 的人参和相容性。有 V 的人参。nigrum L。(G-V ) 用 electrospray 电离团 spectrometry (ESIMS ) 和 UPLCMS n , 分别地。大多数人参皂甙的相对内容在 G-V 的摘录被减少。而且,人参皂甙 Rb 1, Rb 2, Rc 和 Rd 能在老鼠被使产生代射变化到 Rd, F2 和 C-K 肠的细菌。在在 10:5, 10:7 和 10:10 和人参皂甙 Rb 1, Rb 2 的新陈代谢的率的比率的 G-V 摘录的 Rb 1, Rb 2 和 Rc 和到 Rd 的 Rc 的新陈代谢的速度,到在所有相容性摘录的 F2 的 Rd 在 P 是比那低的。人参摘录。在结论,这研究说明了机制由相对内容的比较和在 P 的相容性以后的人参皂甙的新陈代谢的侧面减少效果。人参和 V。nigrum L。 | Xiudong Yang Xue Li Ying Xu Zifeng Pi Na Lin Zhiqiang Liu Fengrui Song | 2015 | Chinese Journal of Chemistry2015，33，9： | 3 |
| 2 | α-Glucosidase immobilization on functionalized Fe_3O_4 magnetic nanoparticles for screening of enzyme inhibitors显示文摘Magnetic nanoparticles(MNPs) are widely used for the immobilization of enzyme owing to the unique properties such as good biocompatibility and rapid separation. Herein, we used Fe_3O_4 magnetic nanoparticles(Fe_3O_4 MNPs) as the carrier core with(3-aminopropyl)triethoxysilane(APTES)modification by our approach, in which a-glucosidase was stereoscopically immobilized on the surface of Fe_3O_4 MNPs via covalent binding. The result of immobilization was characterized by scanning electron microscope(SEM) and fourier transform-infrared spectroscopy(FT-IR). Then we optimized some key parameters of the immobilization reaction, including the ratio of MNPs to enzyme, GA concentration,crosslinking time and immobilization time. Moreover, under the optimal conditions, pH tolerance,thermo stability and reusability of the immobilized enzyme were investigated and compared with the free one. In order to evaluate the change of the affinity of the enzyme to its specific substrate after immobilization, the Michaelis-Menten constant(K_m) was also studied. Finally, the immobilized α-glucosidase combining with high performance liquid chromatography-tandem mass spectrometry technique(HPLC-MS/MS) was applied to screen and identify eight inhibitors from Polygonum cuspidatum extract. These results indicated that the established method had the broad prospects for biotechnological applications. | Guorong Cheng Junpeng Xing Zifeng Pi Shu Liu Zhiqiang Liu Fengrui Song | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 3 | Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles显示文摘Enrichment of trace bioactive constituents and metabolites from complex biological samples is challenging.This study presented a one-pot synthesis of magnetic polydopamine nanoparticles(Fe3O4@-SiO2@PDA NPs)with multiple recognition sites for the magnetic dispersive solid-phase extraction(MDSPE)of ginsenosides from rat plasma treated with white ginseng.The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a highresolution mass spectrometry with supplemental UNIFI libraries.Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides.The reusability of Fe3O4@-SiO2@PDA NPs was also examined and the results showed that the recovery rate exceeded 80%after recycling 6 times.Furthermore,the proposed method showed greater enrichment efficiency and could rapidly determine and characterize 23 ginsenoside prototypes and metabolites from plasma.In comparison,conventional methanol method can only detect 8 ginsenosides from the same plasma samples.The proposed approach can provide methodological reference for the trace determination and characterization of different bioactive ingredients and metabolites of traditional Chinese medicines and food. | Ningning Zhao Shu Liu Junpeng Xing Zifeng Pi Fengrui Song Zhiqiang Liu | 2020 | Journal of Pharmaceutical Analysis2020，10，1： | 1 |
| 4 | Differentiation of various kinds of Fructus schisandrae by surface desorption atmospheric pressure chemical ionization mass spectrometry combined with Principal Component Analysis显示文摘 | Zifeng Pi Hao Yue Li Ma Liying Ding Zhiqiang Liu Shuying Liu | 2011 | Analytica Chimica Acta2011，，2： | 1 |
| 5 | Effect of ginseng polysaccharide on the urinary excretion of type 2 diabetic rats studied by liquid chromatography–mass spectrometry显示文摘 | Jun Niu Zifeng Pi Hao Yue Yang Wang Qing Yu Shuying Liu | 2012 | Journal of Chromatography B2012，，： | 1 |
| 6 | Qualitative and Quantitative Analysis of Drug Interactions: Fritillary Mediating the Transport of Alkaloids in Caco-2 Cells by P-Glycoprotein显示文摘 | MA Jingwei KAN Hong MA Yinghui MEN Lihui PI Zifeng LIU Zhiqiang LIU Zhongying | 2014 | Chemical Research in Chinese Universities2014，30，5： | 1 |
| 7 | Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method显示文摘 | MA Yinghui LIU Yuanyuan WEI Mengying SONG Fengrui LIU Zhongying PI Zifeng | 2015 | Chemical Research in Chinese Universities2015，31，6： | 0 |
| 8 | Effects of the ambient fine particulate matter(PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS显示文摘In order to study the damage of ambient fine particulate matter(PM2.5) to organism in the environment,the method of urinary metabolomics was used to detect the potential endogenous metabolites and their related metabolic pathways to clarify the toxicity mechanisms. Particles of PM2.5 collected from the fibrous membrane were extracted ultrasonically and dissolved in physiological saline to prepare PM2.5 suspension. The solution of saline and fine particular matter was injected into the trachea of rats respectively. The rats were injected two times a week for four weeks. Samples of 24-h urine were collected at the 14 th day after the end of exposure, and the ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used for the metabolomics detection. Principal component analysis(PCA) was used to investigate the global metabolomic alterations and a clear separation in the scatter diagram were observed. 17 potential endogenous metablites were identified from urinary samples in rats by UPLC-Q-TOF-MS. The primary metabolism pathways involved pentose and glucuronate interconversions, starch and sucrose metabolism, tryptophan metabolism, tyrosine metabolism, phenylalanine metabolism, purine metabolism, acetaminophen metabolism pathway, retinol metabolism and valproic acid metabolism pathway.The results are helpful to understand the toxicological mechanisms of PM2.5 and screened out potential biomarkers of rats which exposure to PM2.5. | Lin Zhang Tengfei Xu Zifeng Pi Meizhu Zheng Fengrui Song Zhiqiang Liu | 2019 | Chinese Chemical Letters2019，30，1： | 0 |
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