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您的检索式：作者名="Zituo Yang"
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| 1 | VNN disease and status of breeding for resistance to NNV in aquaculture显示文摘Viral nervous necrosis(VNN)disease,caused by the nervous necrosis virus(NNV),is a devastating disease,leading to high mortality rate and huge economical loss in aquaculture.In the past 30 years,many studies on the virus,host responses to the virus infection and diagnostics have yielded a lot of knowledge on developing measures to prevent the VNN disease.Although genetic improvement of disease resistance through breeding is inheritable and has long-lasting positive effect on aquaculture,it is a very challenging task in conventional selective breeding for improving disease resistance.With the advances in mapping quantitative trait loci(QTL)and genome wide association studies(GWAS)for NNV resistance in aquaculture species,DNA markers and genes associated with NNV resistance have been identified,making the application of marker-assisted selection(MAS)and genomic selection(GS)for NNV resistance possible.A few genes for NNV resistance are identified and are being used for genome editing to rapidly improve NNV resistance.In this review,we present the current knowledge on the NNV,host responses to NNV infection,diagnostic methods and vaccines available for NNV disease.In addition,we summarize the current status of conventional and molecular breeding for NNV resistance and highlight future directions,including genome editing for improving NNV resistance in aquaculture. | Zituo Yang Gen Hua Yue Sek-Man Wong | 2022 | Aquaculture and Fisheries2022，7，2： | 0 |
| 2 | LAMP for the rapid diagnosis of iridovirus in aquaculture显示文摘Iridoviruses are DNA virus and have caused huge economic losses in the aquaculture industry.The aim of this study was to establish a colorimetric loop-mediated isothermal amplification(LAMP)protocol for the on-site detection of Singapore grouper iridovirus(SGIV).The SGIV-VP61 gene was chosen as the target gene to develop a colorimetric LAMP assay.The optimized condition of the colorimetric LAMP assay was incubation at 63℃ for 1 h.Samples infected with SGIV could be detected with the color change from yellow into pink.The sensitivity of the developed assay is 5.66 copies/μL of the viral DNA template.This sensitivity was about 1000 times higher than that of conventional PCR while it was slightly lower than the one-step semi-nested PCR assay.A total of 60 DNA samples extracted from the fin tissue of the SGIV-infected Asian seabass were examined for SGIV by colorimetric LAMP,semi-nested PCR and conventional PCR.The results of the colorimetric LAMP assay showed 94.87%agreement with the semi-nested PCR.In addition,the DNA extraction method using NaOH showed a better performance in the colorimetric LAMP assay.Taken together,the colorimetric LAMP established was a sensitive,rapid and specific method for the detection of SGIV.SGIV was not detected in samples randomly taken from a genetically improved line of the Asian seabass.However,some seabass obtained from the local markets were found to contain SGIV.Thus,the LAMP assay has the potential application in the diagnosis of iridovirus diseases in the aquaculture industry. | Yepin Yu Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Qiwei Qin Gen Hua Yue | 2022 | Aquaculture and Fisheries2022，7，2： | 0 |
| 3 | A chromosome-level genome assembly of chia provides insights into high omega-3 content and coat color variation of its seeds显示文摘Chia(Salvia hispanica)is a functional food crop for humans.Although its seeds contain high omega-3 fatty acids,the seed yield of chia is still low.Genomic resources available for this plant are limited.We report the first high-quality chromosome-level genome sequence of chia.The assembled genome size was 347.6 Mb and covered 98.1%of the estimated genome size.A total of 31069 protein-coding genes were predicted.The absence of recent whole-genome duplication and the relatively low intensity of transposable element expansion in chia compared to its sister species contribute to its small genome size.Transcriptome sequencing and gene duplication analysis reveal that the expansion of the fab2 gene family is likely to be related to the high content of omega-3 in seeds.The white seed coat color is determined by a single locus on chromosome 4.This study provides novel insights into the evolution of Salvia species and high omega-3 content,as well as valuable genomic resources for genetic improvement of important commercial traits of chia and its related species. | Le Wang May Lee Fei Sun Zhuojun Song Zituo Yang Gen Hua Yue | 2022 | Plant Communications2022，3，4： | 0 |
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