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| 1 | 蓖麻Na^+/H^+逆向转运蛋白基因NhaD克隆与表达载体构建显示文摘为发掘耐盐相关基因,以蓖麻叶片为材料,设计特异性引物,克隆蓖麻Na^+/H^+逆向转运蛋白基因(NhaD),并对该序列进行生物信息学分析。结果表明,NhaD全长1 743 bp,可编码580个氨基酸。将其命名为Rc NhaD。其编码蛋白的分子量为61.821 kD,等电点(pI)值6.74。利用DNAMAN软件对该序列与其它植物氨基酸序列进行同源性比对,结果显示与木薯等植物的同源性在85%以上,该蛋白含有ArsB蛋白的功能域,属于ArsB/NhaD转运蛋白家族成员。对其二级结构分析显示该蛋白为疏水性蛋白,该蛋白具有11个跨膜区域,是典型的跨膜蛋白。根据Rc NhaD全长设计带有KpnⅠ与XbaⅠ酶切位点的引物扩增出序列全长,用KpnⅠ与XbaⅠ进行双酶切后与表达载体pCG-1301连接,成功构建了Rc NhaD的表达载体。本研究为进一步研究该基因功能提供指导。 | 张丽雪 王晓宇 李平 李惠根 丛娇娇 张继星 | 2019 | 基因组学与应用生物学2019，38，7： | 1 |
| 2 | Effect of salt stress on the expression of NHX-type Na^+/H^+ antiporters in Populus euphratica and P.pruinosa calli显示文摘Populus euphratica and Populus pruinosa,sister species in the Turanga Section(Salicaceae),growing in semi-arid saline areas are known for their high salinity tolerance.In this study,by combining growth level with Na+and K+contents,the expression level of vacuolar Na+/H+antiporters was investigated for NaCl-induced changes in P.euphratica and P.pruinosa calli.Compared to P.euphratica,P.pruinosa calli grew well in 200 mM NaCl stress from 14 to 21 days.Increasing the stressed time caused an increase in Na+content concomitant with a decrease in K+content in P.euphratica calli,whereas,with the presence of 200 mM NaCl,K+content has a less increase in 14 and 21 days than in 7 days which was detected in P.pruinosa calli.The transcript levels of six genes coding for NHX-type Na+/H+antiporters suggest that vacuolar NHX1–NHX6 antiporters play important roles in responding to salt stress in P.pruinosa.Our data suggest that there exists a higher salt tolerance for P.pruinosa than P.euphratica at the cellular level,Na+avoidance or accumulation is observed in cellular compartments,and that expression of NHX antiporters is linked to the accumulator phenotype. | LiHua Hu YuXia Wu | 2014 | Research in Cold and Arid Regions2014，6，1： | 0 |
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