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| 1 | 骨组织工程支架材料在骨缺损修复及3D打印技术中的应用显示文摘背景:骨组织工程在骨缺损修复方面起着非常重要的作用,可呈递生物活性物质,促进骨组织生长,进而修复骨缺损,其中,支架是骨组织工程的三要素之一,3D打印技术可以实现骨组织修复的个体化治疗,从而个性化定制人工骨。目的:分析几种常用的骨组织工程支架材料的生物学特性,探讨3D打印技术在骨组织工程支架构建中的应用。方法:通过检索2005至2016年Pub Med和万方数据库有关骨组织工程支架材料和3D打印技术应用的相关文献。以'组织工程支架,骨缺损,高分子材料,陶瓷材料,金属材料,复合材料,3D打印'为中文检索词,'Tissue Engineering Scaffold,bone defects,polymer materials,Bioceramics,Metal Materials,Composite Materials,3D printing'为中文检索词,检索摘要内同时包含上述检索词的文献,排除重复性研究,最终纳入65篇文献进一步分析。结果与结论:(1)目前常用的骨组织工程支架材料包括聚合物材料(如天然聚合物材料和高分子聚合物材料)、生物陶瓷和金属材料等,根据材料的特性将不同材料复合可以弥补单一材料的缺点,并制备新型的组织工程支架材料;(2)用于组织工程骨制作的3D打印技术包括熔融沉积技术、选择性激光烧结技术、低温沉积制造技术等;(3)3D打印应用于骨组织工程支架构建时,所用的粉末或黏合剂需具备一定的条件,如流动性、稳定性与可湿性等;用于打印的粉末材料可分为人工合成多聚体、天然高分子聚合物、生物陶瓷及它们的混合物;不同粉末材料的粉末各自优缺点不同,且最终成型效果也不尽相同;(4)3D打印技术制作的骨组织工程支架在力学、结构、个性化方面有其独特的优势,在骨支架制造方面有广泛的应用前景。 | 党莹 李月 李瑞玉 吴立萍 郭雅静 宋瑞佳 | 2017 | 中国组织工程研究2017，21，14： | 35 |
| 2 | 农业增长方式转变中的农业投资问题研究显示文摘 | 杨明洪 | 2000 | 投资研究2000，19，4： | 30 |
| 3 | Osteoimmunomodulatory effects of biomaterial modification strategies on macrophage polarization and bone regeneration显示文摘Biomaterials as bone substitutes are always considered as foreign bodies that can trigger host immune responses.Traditional designing principles have been always aimed at minimizing the immune reactions by fabricating inert biomaterials.However,clinical evidence revealed that those methods still have limitations and many of which were only feasible in the laboratory.Currently,osteoimmunology,the very pioneering concept is drawing more and more attention-it does not simply regard the immune response as an obstacle during bone healing but emphasizes the intimate relationship of the immune and skeletal system,which includes diverse cells,cytokines,and signaling pathways.Properties of biomaterials like topography,wettability,surface charge,the release of cytokines,mediators,ions and other bioactive molecules can impose effects on immune responses to interfere with the skeletal system.Based on the bone formation mechanisms,the designing methods of the biomaterials change from immune evasive to immune reprogramming.Here,we discuss the osteoimmunomodulatory effects of the new modification strategies—adjusting properties of bone biomaterials to induce a favorable osteoimmune environment.Such strategies showed potential to benefit the development of bone materials and lay a solid foundation for the future clinical application. | Yajuan Xie Cheng Hu Yi Feng Danfeng Li Tingting Ai Yulei Huang Xiaodan Chen Lijia Huang Jiali Tan | 2020 | Regenerative Biomaterials2020，7，3： | 22 |
| 4 | Poly(lactic-co-glycolic acid)-based composite bone-substitute materials显示文摘Research and development of the ideal artificial bone-substitute materials to replace autologous and allogeneic bones for repairing bone defects is still a challenge in clinical orthopedics.Recently,poly(lactic-co-glycolic acid)(PLGA)-based artificial bone-substitute materials are attracting increasing attention as the benefit of their suitable biocompatibility,degradability,mechanical properties,and capabilities to promote bone regeneration.In this article,we comprehensively review the artificial bone-substitute materials made from PLGA or the composites of PLGA and other organic and inorganic substances,elaborate on their applications for bone regeneration with or without bioactive factors,and prospect the challenges and opportunities in clinical bone regeneration. | Duoyi Zhao Tongtong Zhu Jie Li Liguo Cui Zhiyu Zhang Xiuli Zhuang Jianxun Ding | 2021 | Bioactive Materials2021，6，2： | 14 |
| 5 | 人工听骨链重建材料生物相容性、特性及修复喉软骨缺损的效果显示文摘背景:喉软骨缺损替代品较多,包括自体软骨、同种异体软骨以及金属支架等,这些材料在喉软骨缺损中均能取得理想效果,但是也存在许多不足。目的:研究人工听骨链重建材料生物相容性及其特性,探讨人工听骨链重建材料在喉软骨缺损中的修复效果。方法:(1)从新鲜人骨中提取骨形态发生蛋白,将羟基磷灰石高温煅烧后制备多孔羟基磷灰石,将获得的多孔羟基磷灰石听骨放入骨形态发生蛋白溶液中,制备骨形态发生蛋白-羟基磷灰石人工听骨链重建材料,分析材料的生物相容性及特性。(2)将40只新西兰雄性成年白兔随机分为多孔羟基磷灰石组和人工听骨链重建材料组,每组20只。制备新西兰雄性成年白兔喉软骨缺损模型,多孔羟基磷灰石组采用多孔羟基磷灰石材料进行修复,人工听骨链重建材料组采用骨形态发生蛋白-羟基磷灰石人工听骨链重建材料修复。结果和结论:(1)不同孔隙率人工听骨重建材料抗压强度和抗压强度差异有显著性意义;(2)骨形态发生蛋白-羟基磷灰石人工听骨链重建材料外表面空洞未形成对称性球状,外观表现为多边形,空隙尺寸为100-200μm;(3)2组大鼠分别植入骨形态发生蛋白-多孔羟基磷灰石和骨形态发生蛋白-羟基磷灰石人工听骨链重建材料后未见明显的不良反应。但人工听骨链重建材料组出现较多的结缔组织及明显骨性结节,且材料降解速度较快。(4)提示骨形态发生蛋白-羟基磷灰石人工听骨链重建材料具有良好的生物相容性和特性,利用其修复喉软骨缺损效果理想,具有较高的临床应用价值。 | 郭蓓 袁琨 陈伟 | 2016 | 中国组织工程研究2016，20，25： | 6 |
| 6 | 构建组织工程软骨支架材料:现状及未来显示文摘背景:众所周知,组织工程修复软骨损伤有种子细胞、支架材料、细胞因子3个要素,支架材料在软骨组织工程制备中发挥着重要作用。目的:检索近十年来国内外关于软骨组织工程支架材料的相关文献,并对其进行归纳总结,讨论支架材料发展遇到的问题及发展方向。方法:由第一作者检索2006至2016年Pub Med数据库及CNKI数据库文献,英文检索词为'cartilage tissue engineering,natural scaffold materials,synthetic scaffold materials,composite scaffolds,nanometer materials',中文检索词为'软骨组织工程,天然支架材料,人工合成支架材料,复合支架材料,纳米支架'。结果与结论:软骨组织工程支架材料来源与种类不一,天然材料、人工材料及纳米材料有各自的优点及缺点,任何单一的材料难以满足临床上对软骨的要求,将不同来源的材料按照不同比例进行复合,扬长避短,能构成力学性能良好、高孔隙率、相容性好、降解性能佳的支架材料。但目前仍存在支架材料降解过快、细胞过度生长等问题,目前相关研究大都样本量较小,缺乏长期跟踪随访,并且人体试验相对较少。 | 周勇 朱伟民 彭亮权 王大明 刘威 徐晓 王大平 | 2017 | 中国组织工程研究2017，21，10： | 6 |
| 7 | 骨软骨组织工程分层支架的研究进展显示文摘因各种因素导致的关节骨软骨缺损的处理至今仍是一个棘手的临床问题。随着组织工程研究的不断深入,过去十年中骨软骨缺损的修复策略及方法获得了很大进展,部分骨软骨组织分层支架产品逐渐步入临床试验阶段。本文通过检索关于组织工程治疗骨软骨缺损的相关文献,纳入有关分层复合支架的相关文章,分析分层复合支架在骨软骨组织工程领域的研究现状及发展前景。单相支架能够支持成骨和软骨细胞黏附和增殖,但缺乏骨软骨再生修复所需要的固有的分层结构特征。双相支架在单相支架的生物相容性基础上,采用两种或多种材料制备支架的软骨相和骨相。双相支架在模拟天然组织结构上较单相支架有明显提高,但构建组织的骨、软骨界面可能存在结合欠佳的问题。三相支架或多相支架在双相支架的基础上,添加中间层模拟正常软骨组织的钙化层,可更好地连接骨层和软骨层,并在动物实验中获得了较好的结果。梯度支架在分层支架的基础上将层与层之间进行梯度连接,更接近天然软骨结构。在近五年的相关研究中,骨软骨分层支架的发展主要依托支架微观结构和制备工艺的创新。骨软骨分层支架应用于临床的研究较少,需要更多的研究探索。 | 康嘉宇 吕建伟 赵志虎 马剑雄 马信龙 | 2019 | 中华骨科杂志2019，39，22： | 4 |
| 8 | 酸性成纤维细胞生长因子复合胶原蛋白对兔前交叉韧带重建后腱-骨愈合的影响显示文摘目的观察酸性成纤维细胞生长因子(a FGF)复合胶原蛋白对兔前交叉韧带(ACL)重建后腱-骨界面愈合的影响。方法将30只新西兰白兔随机分为实验组与对照组,每组15只,均取趾长伸肌腱作为移植物。实验组将a FGF/胶原蛋白复合物植入到重建的ACL腱-骨界面,对照组单纯行ACL重建。于术后第4、8、12周分别处死动物取材,将股骨与胫骨端分别固定于生物力学试验机上,测试移植肌腱的腱-骨界面抗拉伸强度,取其绝对值作比较;同时,将股骨隧道和胫骨隧道纵向剖开,取下标本组织学观察移植物界面愈合情况。结果术后第4、8、12周抗拉力强度实验组强于对照组,差异有统计学意义(P<0.001)。两组动物均在第4周时移植物与骨隧道之间均形成了纤维结缔组织。术后第12周,实验组肌腱移植物在骨隧道内形成了纤维软骨移行带,类似于正常的纤维软骨连接,而对照组则形成了走向与骨隧道轴向垂直的Sharpey样纤维。结论a FGF复合胶原蛋白能促进兔ACL重建腱-骨界面的早期直接愈合。 | 逯代锋 杨传东 董锋 周勇 严实 | 2017 | 临床骨科杂志2017，20，6： | 4 |
| 9 | 人牙髓细胞在可注射PLGA微球支架上的粘附生长显示文摘目的:探讨不同密度的人牙髓细胞(HDPCs)在可注射聚乳酸/羟基乙酸(PLGA)微球支架上的粘附生长情况。方法:取第4代HDPCs,以3种不同密度(2×10~5/mL、1×10~5/mL、5×10~4/mL)接种于PLGA微球(A、B、C组),于不同时间点倒置显微镜下观察细胞粘附生长情况;检测各组细胞双链脱氧核糖核酸(dsDNA)含量及碱性磷酸酶(ALP)活性。体外培养8周后,扫描电镜观察HDPCs和PLGA微球材料的形貌结构。结果:倒置显微镜下可见细胞粘附于微球材料,随着时间延长,细胞增殖情况良好,与材料融合生长。培养1周,3组细胞dsDNA含量及ALP活性均无显著差异;培养2周,A、B两组细胞dsDNA含量显著高于C组,B组ALP活性水平最高。培养8周,扫描电镜可见细胞与微球完全融合,表面有基质样物质沉积,局部有凹陷形成。结论:1×10~5/mLHDPCs接种于PLGA微球,细胞dsDNA含量及ALP活性水平较高。PLGA微球作为一种可注射支架材料,有利于HDPCs粘附生长,可望用于构建组织工程化牙髓。 | 王冠华 宋芳 石旭东 邹慧儒 | 2017 | 牙体牙髓牙周病学杂志2017，27，7： | 3 |
| 10 | PLGA生物可降解膜用于预防宫腔粘连的初步研究显示文摘目的:研究聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]膜作为生物可降解材料用于预防宫腔粘连的可行性初步研究。方法:制备不同单体比例的PLGA膜,分别于模拟宫腔液以及昆明小鼠宫腔中进行体外和体内降解实验。将30只昆明小鼠随机分为假手术组、宫腔粘连组、PLGA膜组,每组各10只,运用Masson染色和Western blot观察各组昆明小鼠子宫内膜纤维化程度和相关蛋白的表达。结果:不同单体比例的PLGA膜体外降解时间不同,PLGA膜(50∶50)的降解速度最快,在4周时质量损失达98.5%;PLGA膜(90∶10)降解速度最慢,在12周时其质量损失仅为7.8%;PLGA(75∶25)降解速度介于前两者之间,于12周时质量损失达95.7%。不同单体比例的PLGA膜体内降解时间不同,PLGA膜(50∶50)7 d后在宫腔内完全降解,而PLGA膜(75∶25)、PLGA膜(90∶10)仍然滞留于宫腔中。与假手术组相比,PLGA膜组(75∶25)增加了昆明小鼠子宫内膜纤维化面积(P=0.000)以及相关蛋白的表达(P<0.01);与宫腔粘连组相比,PLGA膜组(75∶25)子宫内膜纤维化面积以及相关蛋白的表达无明显差异(P>0.05)。结论:PLGA生物可降解材料用于预防宫腔粘连,具有宫腔内降解速度可调节、不增加宫腔粘连组子宫内膜纤维化程度的特点,在预防宫腔粘连方面具有潜在的应用价值。 | 朱轶 胡丽娜 任艳 | 2017 | 重庆医科大学学报2017，42，4： | 3 |
| 11 | 软骨脱细胞基质-Ⅱ型胶原制备组织工程软骨纳米支架的实验研究显示文摘目的探索软骨脱细胞基质CAEM-Ⅱ型胶原(COLⅡ)通过静电纺丝法制备组织工程纳米支架的可行性。方法将兔肋软骨脱细胞、脱脂、酶解后干燥获得CAEM,再将CAEM和COLⅡ按质量比1∶2的比例混合,通过静电纺丝技术制备组织工程纳米支架,通过测定其吸水率、降解率等检测支架的理化性能,用CCK8法评价其细胞毒性及粘附性情况。结果 CAEM-COLⅡ纳米支架纤维直径为(627±165.4)nm,吸水率为(623.0±27.4)%,35天降解率为(45.6±5.8)%,CCK8法检测结果显示CAEM-COLⅡ复合支架对软骨细胞具有良好的粘附性,生物学性能良好。结论CAEM-COLⅡ纳米支架能为软骨细胞的生长和增殖提供优良的微环境,在组织工程软骨重建中具有潜在的应用价值。 | 蒋婷 杨泽龙 李小兵 冯刚 | 2016 | 西部医学2016，28，8： | 3 |
| 12 | RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering显示文摘In recent years,much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular behaviors.In our study,RGD peptide and graphene oxide(GO)co-functionalized poly(lactide-co-glycolide,PLGA)(RGD-GO-PLGA)nanofiber mats were fabricated via electrospinning,and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue engineering.Scanning electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of randomoriented electrospun nanofibers with average diameter of 558nm.The successful co-functionalization of RGD peptide and GO into the PLGA nanofibers was confirmed by Fourier-transform infrared spectroscopic analysis.Moreover,the surface hydrophilicity of the nanofiber mats was markedly increased by co-functionalizing with RGD peptide and GO.It was found that the mats were thermally stable under the cell culture condition.Furthermore,the initial attachment and proliferation of primarily cultured vascular smoothmuscle cells(VSMCs)on the RGD-GO-PLGA nanofibermats were evaluated.It was revealed that the RGD-GO-PLGA nanofibermats can effectively promote the growth of VSMCs.In conclusion,our findings suggest that the RGD-GO-PLGA nanofiber mats can be promising candidates for tissue engineering scaffolds effective for the regeneration of vascular smooth muscle. | Yong Cheol Shin Jeonghyo Kim Sung Eun Kim Su-Jin Song SuckWon Hong Jin-Woo Oh Jaebeom Lee Jong-Chul Park Suong-Hyu Hyon Dong-Wook Han | 2017 | Regenerative Biomaterials2017，4，3： | 3 |
| 13 | Coordination Insertion Mechanism of Ring-Opening Polymerization of Lactide Catalyzed by Stannous Octoate显示文摘Main observation and conclusion Ring-opening polymerization(ROP)of cyclic esters in the presence of stannous octoate(Sn(Oct)2)is the main way to obtain biodegradable aliphatic polyesters,an important family of biodegradable polymers which have been widely used and still rapidly developed in the fields of biomedical polymers and environment-friendly materials. | Weihan Rao Caiyun Cai Jingyu Tang Yiman Wei Caiyun Gao Lin Yu Jiandong Ding | 2021 | Chinese Journal of Chemistry2021，39，7： | 2 |
| 14 | Magnetic resonance imaging for non-invasive clinical evaluation of normal and regenerated cartilage显示文摘With the development of tissue engineering and regenerative medicine,it is much desired to establish bioimaging techniques to monitor the real-time regeneration efficacy in vivo in a non-invasive way.Herein,we tried magnetic resonance imaging(MRI)to evaluate knee cartilage regeneration after implanting a biomaterial scaffold seeded with chondrocytes,namely,matrix-induced autologous chondrocyte implantation(MACI).After summary of the T2 mapping and the T1-related delayed gadolinium-enhanced MRI imaging of cartilage(dGEMRIC)in vitro and in vivo in the literature,these two MRI techniques were tried clinically.In this study,18 patients were followed up for 1 year.It was found that there was a significant difference between the regeneration site and the neighboring normal site(control),and the difference gradually diminished with regeneration time up to 1 year according to both the quantitative T1 and T2 MRI methods.We further established the correlation between the quantitative evaluation of MRI and the clinical Lysholm scores for the first time.Hence,the MRI technique was confirmed to be a feasible semiquantitative yet non-invasive way to evaluate the in vivo regeneration of knee articular cartilage. | Xian Xu Jingming Gao Shuyun Liu Liang Chen Min Chen Xiaoye Yu Ning Ma Jun Zhang Xiaobin Chen Lisen Zhong Lin Yu Liming Xu Quanyi Guo Jiandong Ding | 2021 | Regenerative Biomaterials2021，8，5： | 2 |
| 15 | Chitosan coatings with distinct innate immune bioactivities differentially stimulate angiogenesis,osteogenesis and chondrogenesis in poly-caprolactone scaffolds with controlled interconnecting pore size显示文摘This study tested whether osseous integration into poly(ε-caprolactone)(PCL)bioplastic scaffolds with fully-interconnecting 155±8μm pores is enhanced by an adhesive,non-inflammatory 99% degree of deacetylation(DDA)chitosan coating(99-PCL),or further incorporation of pro-inflammatory 83% DDA chitosan microparticles(83-99-PCL)to accelerate angiogenesis.New Zealand White rabbit osteochondral knee defects were press-fit with PCL,99-PCL,83-99-PCL,or allowed to bleed(drill-only).Between day 1 and 6 weeks of repair,drill-only defects repaired by endochondral ossification,with an 8-fold higher bone volume fraction(BVF)versus initial defects,compared to a 2-fold(99-PCL),1.1-fold(PCL),or 0.4-fold(83-99-PCL)change in BVF.Hematoma innate immune cells swarmed to 83-99-PCL,elicited angiogenesis throughout the pores and induced slight bone resorption.PCL and 99-PCL pores were variably filled with cartilage or avascular mesenchyme near the bone plate,or angiogenic mesenchyme into which repairing trabecular bone infiltrated up to 1 mm deep.More repair cartilage covered the 99-PCL scaffold(65%)than PCL(18%)or 83-99-PCL(0%)(p<0.005).We report the novel finding that non-inflammatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold,and were compatible with trabecular bone integration.This study also revealed that in vitro osteogenesis assays have limited ability to predict osseous integration into porous scaffolds,because(1)in vivo,woven bone integrates from the leading edge of regenerating trabecular bone and not from mesenchymal cells adhering to scaffold surfaces,and(2)bioactive coatings that attract inflammatory cells induce bone resorption. | Caroline D.Hoemann Javier Rodríguez Gonzalez Jessica Guzman-Morales Gaoping Chen Ebrahim Jalali Dil Basil D.Favis | 2022 | Bioactive Materials2022，7，4： | 1 |
| 16 | Synthesis of photo-reactive poly(vinyl alcohol)and construction of scaffold-free cartilage like pellets in vitro显示文摘Photo-reactive poly(vinyl alcohol)(PRPVA)was synthesized by introduction of phenyl azido groups into poly(vinyl alcohol)(PVA)and applied for surface modification.PRPVA was grafted onto cell culture plate surface homogeneously or in a micropattern.Human mesenchymal stem cells(hMSCs)cultured on cell culture plate surface and PVA-modified surface showed different behaviors.Cells adhered and spread well on cell culture plate surface,while they did not adhere on PVA-grafted surface at all.When hMSCs were cultured on PVA-micropatterned surface,they formed a cell micropattern.Cells formed pellets after cultured on PVA homogeneously modified surface in chondrogenic induction medium for 2 weeks.The pellets were positively stained by hematoxylin/eosin,safranin-O/fast green and toluidin blue,and they were also stained brown by Type II collagen and proteoglycan immunohistological staining.Real-time PCR analysis was conducted to investigate the expression of colI,colII,colX,aggrecan and sox9 mRNA.Results of gene expression were in agreement with those of histological and immunohistological observations.These results indicated that hMSCs cultured on PVA-modified surface performed chondrogenic differentiation,and it was possible to construct scaffold-free cartilage like pellets with PVA-modified surface in vitro. | Bao Li Yongli Gao Likun Guo Yujiang Fan Naoki Kawazoe Hongsong Fan Xingdong Zhang Guoping Chen | 2018 | Regenerative Biomaterials2018，5，3： | 1 |
| 17 | 软骨组织工程中支架材料研究的新进展显示文摘关节软骨损伤是骨科较为常见的一种疾病。目前,骨软骨缺损的处理方法主要有自体移植、微骨折术、软骨下骨钻孔术和自体软骨细胞移植,这些方法都有各自的局限性,均不能完全满足临床以及患者预后的运动要求。20世纪90年代,组织工程的概念第一次提出,这是一个综合工程学与生命科学的跨学科领域,其目的是开发生物替代品,修复、维持与改善组织的功能,甚至重新打造整个器官。 | 吴琪 亓建洪 | 2017 | 中国医药生物技术2017，12，2： | 1 |
| 18 | 制备脱细胞全喉-下咽-食管复合支架用于咽喉重建的实验研究显示文摘目的制备新型脱细胞全喉-下咽-食管复合支架,并探讨其作为咽喉重建替代材料的可行性。方法通过成年新西兰大白兔颈总动脉灌注去离子剂制备脱细胞全喉-下咽-食管复合支架,并行HE染色、扫描电镜(SEM)、免疫原性检测及生物相容性分析;采用密度梯度离心法分离培养兔骨髓间充质干细胞(BMSCs),CCK8法分析支架浸提液的毒性作用,并将细胞复合于脱细胞支架上,体内埋植1周后观察细胞在支架上的粘附及增殖情况。结果HE染色显示脱细胞全喉-下咽-食管支架除软骨以外的其他组织均仅剩细胞外基质(ECM),无细胞成分及结构残留,SEM电镜显示ECM的胶原纤维束,呈蜂窝状,无细胞结构,CCK8提示支架浸提液对细胞生长无明显毒性作用,且细胞可在支架上成功粘附并生长。结论通过灌注法制备低免疫原性的保留ECM的脱细胞全喉-下咽-食管支架具有可行性。 | 吕蝶 侯楠 徐小丽 卢彦青 李京芝 马瑞娜 崔鹏程 | 2021 | 中国耳鼻咽喉颅底外科杂志2021，27，5： | 0 |
| 19 | 保留软骨的去细胞全喉支架制备方法及在喉软骨缺损中的修复效果显示文摘目的探讨保留软骨的去细胞全喉支架的制备方法及在大鼠喉软骨缺损中的修复效果。方法取6只大鼠用于支架的制备,分离颈总动脉,结扎分支,经颈总动脉灌注去污剂(SDS、Triton X-100、PBS离子型),3只大鼠去除喉黏膜、肌肉、韧带的同时,保留细胞外基质和软骨细胞制备保留软骨的去细胞全喉支架,其余3只用于制备对照组支架。另取34只大鼠,建立0.5 cm×0.5 cm全层喉软骨缺损模型,根据处理方法不同随机分为对照组(n=17)和保留软骨组(n=17),对照组植入未经处理的异体喉骨支架,保留软骨组植入保留软骨的去细胞全喉支架,比较两组的修复效果。结果 (1)两种支架经过脱细胞灌注处理后,全喉大体结构完整,体积未发生明显的变化;(2)保留软骨的去细胞全喉支架未见表面细胞组织填充,可见胶原、细胞外基质成分组成的腔隙,细胞之间结构紧密,存在于会厌、声带、环状软骨等表面;对照组支架组织中的细胞成分被完全去除,但是依稀可见网状连接,结构之间不紧密;(3)免疫荧光结果显示:保留软骨的去细胞全喉支架除了有肌肉、软骨细胞外,主要组织相容性复合体(MHC)-Ⅰ、MHC-Ⅱ染色均为阴性;对照组支架免疫荧光染色下MHC-Ⅰ、MHC-Ⅱ染色均为阳性;(4)保留软骨组大鼠修复6 w后缺损部位存在少许不成熟的软骨细胞,极少的软骨形成陷窝,表面存在纤维组织及炎症细胞;修复12 w后缺损部位得到修复,形成软骨陷窝,炎症反应减少;对照组修复6 w后缺损部位周围软骨细胞较少,缺损部位与周围组织边界明显,存在大量炎症细胞;修复12 w后存在少量纤维组织,存在炎症因子。结论保留软骨组植入保留软骨的去细胞全喉支架在喉软骨缺损中具有良好的修复效果。 | 苏莉莎 彭涛 冯俊 李红光 李志勇 杜经纬 马鹏 吉爽 | 2017 | 中国老年学杂志2017，37，24： | 0 |
| 20 | 脐血移植及临床应用显示文摘 | 弓志国 | 2000 | 实用医学进修杂志2000，28，1： | 0 |
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