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| 1 | The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic显示文摘Osteoinductivity of porous calcium phosphate(CaP)ceramics has been widely investigated and confirmed,and it might be attributed to the rapid formation of the vascular networks after in vivo implantation of the ceramics.In this study,to explore the vascularization mechanism within the CaP ceramics,the migration and differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)under the stimulation of porous biphasic calcium phosphate(BCP)ceramic with excellent osteoinductivity were systematically investigated.The results indicated that the directional migration of BMSCs toward BCP ceramic occurred when evaluated by using a transwell model,and the BMSCs migration was enhanced by the seeded macrophages on the ceramic in advance.Besides,by directly culturing BMSCs on BCP ceramic discs under both in vitro and in vivo physiological environment,it was found that the differentiation of BMSCs toward vascular endothelial cells(VECs)happened under the stimulation of BCP ceramic,as was confirmed by the up-regulated gene expressions and protein secretions of VECs-related characteristic factors,including kinase insert domain receptor,von willebrand factor,vascular cell adhesion molecule-1 and cadherin 5 in the BMSCs.This study offered a possibility for explaining the origin of VECs during the rapid vascularization process after in vivo implantation of porous CaP ceramics and could give some useful guidance to reveal the vascularization mechanism of the ceramics. | Ying Chen Jing Wang Xiangdong Zhu Xuening Chen Xiao Yang Kai Zhang Yujiang Fan Xingdong Zhang | 2018 | Regenerative Biomaterials2018，5，3： | 8 |
| 2 | Oral health in China: from vision to action显示文摘Chinese president Xi Jinping made clear at the National Health and Wellness Conference that health is the prerequisite for people's all-around development and a precondition for the sustainable development of China. Oral health is an indispensable component of overall health in humans. However, the long neglect of oral health in overall health agendas has made oral diseases an increasing concern. With this perspective, we described the global challenges of oral diseases, with an emphasis on the challenges faced by China. We also described and analyzed the recently released health policies of the Chinese government, which aim to guide midterm and long-term oral health promotion in China. More importantly, we called for specific actions to fulfill the larger goal of oral health for the nation. The implementation of primordial prevention efforts against oral diseases, the integration of oral health into the promotion of overall health, and the management of oral diseases in conjunction with other chronic non-communicable diseases with shared risk factors were highly recommended. In addition, we suggested the reform of standard clinical residency training, the development of domestic manufacturing of dental equipment and materials, the revitalization traditional Chinese medicine for the prevention and treatment of oral diseases, and integration of oral health promotion into the Belt and Road Initiative. We look forward to seeing a joint effort from all aspects of the society to fulfill the goal of Healthy China 2030 and ensure the oral health of the nation. | Xuedong Zhou Xin Xu Jiyao Li Deyu Hu Tao Hu Wei Yin Yujiang Fan Xingdong Zhang | 2018 | International Journal of Oral Science2018，10，1： | 7 |
| 3 | 3D printing of calcium phosphate bioceramic with tailored biodegradation rate for skull bone tissue reconstruction显示文摘The bone regenerative scaffold with the tailored degradation rate matching with the growth rate of the new bone is essential for adolescent bone repair.To satisfy these requirement,we proposed bone tissue scaffolds with controlled degradation rate using osteoinductive materials(Ca-P bioceramics),which is expected to present a controllable biodegradation rate for patients who need bone regeneration.Physicochemical properties,porosity,compressive strength and degradation properties of the scaffolds were studied.3D printed Ca-P scaffold(3DS),gas foaming Ca-P scaffold(FS)and autogenous bone(AB)were used in vivo for personalized beagle skull defect repair.Histological results indicated that the 3DS was highly vascularized and well combined with surrounding tissues.FS showed obvious newly formed bone tissues.AB showed the best repair effect,but it was found that AB scaffolds were partially absorbed and degraded.This study indicated that the 3D printed Ca-P bioceramics with tailored biodegradation rate is a promising candidate for personalized skull bone tissue reconstruction. | Boqing Zhang Huan Sun Lina Wu Liang Ma Fei Xing Qingquan Kong Yujiang Fan Changchun Zhou Xingdong Zhang | 2019 | Bio-Design and Manufacturing2019，2，3： | 5 |
| 4 | Fluid Property Discrimination in Dolostone Reservoirs Using Well Logs显示文摘The Ordovician Majiagou Formation is one of the main gas-producing strata in the Ordos Basin,China.The identification of hydrocarbon-bearing intervals via conventional well logs is a challenging task.This study describes the litholog of Ma 5(Member 5 of Majiagou Formation)dolostones,and then analyzes the responses of various conventional well logs to the presences of natural gas.The lithology of the gas bearing layers is dominantly of the dolomicrite to fine to medium crystalline dolomite.Natural gas can be produced from the low resistivity layers,and the dry layers are characterized by high resistivities.Neutron-density crossovers are not sensitive to the presences of natural gas.In addition,there are no significant increases in sonic transit times in natural gas bearing layers.NMR(nuclear magnetic resonance)logs,DSI(Dipole Sonic Imager)logs and borehole image logs(XRMI)are introduced to discriminate the fluid property in Majiagou dolostone reservoirs.The gas bearing intervals have broad NMR T2(transverse relaxation time)spectrum with tail distributions as well as large T2gm(T2 logarithmic mean values)values,and the T2 spectrum commonly display polymodal behaviors.In contrast,the dry layers and water layers have low T2gm values and very narrow T2 spectrum without tails.The gas bearing layers are characterized by low Vp/Vs ratios,low Poisson’s ratios and low P-wave impedances,therefore the fluid property can be discriminated using DSI logs,and the interpretation results show good matches with the gas test data.The apparent formation water resistivity(AFWR)spectrum can be derived from XRMI image logs by using the Archie’s formula in the flushed zone.The gas bearing layers have broad apparent formation water resistivity spectrum and tail distributions compared with the dry and water layers,and also the interpretation results from the image logs exhibit good agreement with the gas test data.The fluid property in Majiagou dolostone reservoirs can be discriminated through NMR logs,DSI logs and borehole image logs.This study helps establish a predictable model for fluid property in dolostones,and have implications in dolostone reservoirs with similar geological backgrounds worldwide. | WANG Guiwen LAI Jin LIU Bingchang FAN Zhuoying LIU Shichen SHI Yujiang ZHANG Haitao CHEN Jing | 2020 | Acta Geologica Sinica(English Edition)2020，94，3： | 4 |
| 5 | BMSCs-assisted injectable Col I hydrogel-regenerated cartilage defect by reconstructing superficial and calcified cartilage显示文摘The self-healing capacity of cartilage was limited due to absence of vascular,nervous and lymphatic systems.Although many clinical treatments have been used in cartilage defect repair and shown a promising repair result in short term,however,regeneration of complete zonal structure with physiological function,reconstruction cartilage homeostasis and maintaining long-term repair was still an unbridgeable chasm.Cartilage has complex zonal structure and multiple physiological functions,especially,superficial and calcified cartilage played an important role in keeping homeostasis.To address this hurdle of regenerating superficial and calcified cartilage,injectable tissue-induced type I collagen(Col I)hydrogel-encapsulated BMSCs was chosen to repair cartilage damage.After 1 month implantation,the results demonstrated that Col I gel was able to induce BMSCs differentiation into chondrocytes,and formed hyaline-like cartilage and the superficial layer with lubrication function.After 3 months post-surgery,chondrocytes at the bottom of the cartilage layer would undergo hypertrophy and promote the regeneration of calcified cartilage.Six months later,a continuous anatomical tidemark and complete calcified interface were restored.The regeneration of neo-hyaline cartilage was similar with adjacent normal tissue on the thickness of the cartilage,matrix secretion,collagen type and arrangement.Complete multilayer zonal structure with physiological function remodeling indicated that BMSCs-assisted injectable Col I hydrogel could reconstruct cartilage homeostasis and maintain long-term therapeutic effect. | Hanxu Cai PeileiWang Yang Xu Ya Yao Jia Liu Tao Li Yong Sun Jie Liang Yujiang Fan Xingdong Zhang | 2020 | Regenerative Biomaterials2020，7，1： | 4 |
| 6 | Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo显示文摘Extracellular matrix(ECM)-based biomaterials are promising candidates in cartilage tissue engineering by simulating the native microenvironment to regulate the chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)without exogenous growth factors.The biological properties of ECM scaffolds are primarily depended on the original source,which would directly influence the chondrogenic effects of the ECM materials.Despite the expanding investigations on ECM scaffolds in recent years,the selection of optimized ECM materials in cartilage regeneration was less reported.In this study,we harvested and compared the articular cartilage ECM from newborn,juvenile and adult rabbits.The results demonstrated the significant differences in the mechanical strength,sulphated glycosaminoglycan and collagen contents of the different aged ECM,before and after decellularization.Consequently,different compositional and mechanical properties were shown in the three ECM-based collagen hydrogels,which exerted age-dependent chondrogenic inducibility.In general,both in vitro and in vivo results suggested that the newborn ECM promoted the most chondrogenesis of BMSCs but led to severe matrix calcification.In contrast,BMSCs synthesized the lowest amount of cartilaginous matrix with minimal calcification with adult ECM.The juvenile ECM achieved the best overall results in promoting chondrogenesis of BMSCs and preventing matrix calcification.Together,this study provides important information to our current knowledge in the design of future ECM-based biomaterials towards a successful repair of articular cartilage. | Xiuyu Wang Yan Lu Wan Wang Qiguang Wang Jie Liang Yujiang Fan Xingdong Zhang | 2020 | Regenerative Biomaterials2020，7，6： | 4 |
| 7 | A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model显示文摘Repair and reconstruction of large bone defect were often difficult,and bone substitute materials,including autogenous bone,allogenic bone and artificial bone,were common treatment strategies.The key to elucidate the clinical effect of these bone repair materials was to study their osteogenic capacity and immunotoxicological compatibility.In this paper,the mechanical properties,micro-CT imaging analysis,digital image analysis and histological slice analysis of the three bone grafts were investigated and compared after different time points of implantation in rat femur defect model.Autogenous bone and biphasic calcium phosphate particular artificial bone containing 61.4% HA and 38.6%β-tricalcium phosphate with 61.64%porosity and 0.8617±0.0068 g/cm^(3) den-sity(d≤2 mm)had similar and strong bone repair ability,but autogenous bone implant materials caused greater secondary damage to experimental animals;allogenic bone exhibited poor bone defect repair ability.At the early stage of implantation,the immunological indexes such as Immunoglobulin G,Immunoglobulin M concentration and CD4 cells'population of allogenic bone significantly increased in compared with those of autologous bone and artificial bone.Although the repair process of artificial bone was relatively inefficient than autologous bone graft,the low immunotoxicological indexes and acceptable therapeutic effects endowed it as an excellent alter-native material to solve the problems with insufficient source and secondary trauma of autogenous bone. | Wen Zou Xing Li Na Li Tianwei Guo Yongfu Cai Xiaoqin Yang Jie Liang Yong Sun Yujiang Fan | 2021 | Regenerative Biomaterials2021，8，1： | 3 |
| 8 | Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds显示文摘Mechanical signals have been played close attention to regulate chondrogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).In this study,dynamic mechanical loading simulation with natural frequencies and intensities were applied to the 3D cultured BMSCs–collagen scaffold constructs.We investigated the effects of dynamic mechanical loading on cell adhesion,uniform distribution,proliferation,secretion of extracellular matrix(ECM)and chondrogenic differentiation of BMSCs–collagen scaffold constructs.The results indicated that dynamic mechanical loading facilitated the BMSCs adhesion,uniform distribution,proliferation and secretion of ECM with a slight contraction,which significantly improved the mechanical strength of the BMSCs–collagen scaffold constructs for better mimicking the structure and function of a native cartilage.Gene expression results indicated that dynamic mechanical loading contributed to the chondrogenic differentiation of BMSCs with higher levels of AGG,COL2A1 and SOX9 genes,and prevented of hypertrophic process with lower levels of COL10A1,and reduced the possibility of fibrocartilage formation due to down-regulated COL1A2.In conclusion,this study emphasized the important role of dynamic mechanical loading on promoting BMSCs chondrogenic differentiation and maintaining the cartilage phenotype for in vitro reconstruction of tissue-engineered cartilage,which provided an attractive prospect and a feasibility strategy for cartilage repair. | Wanxu Cao Weimin Lin Hanxu Cai Yafang Chen Yi Man Jie Liang Qiguang Wang Yong Sun Yujiang Fan Xingdong Zhang | 2019 | Regenerative Biomaterials2019，6，2： | 3 |
| 9 | Smad5 acts as an intracellular pH messenger and maintains bioenergetic homeostasis显示文摘环境暗示和细胞内部的 bioenergetic 状态深刻地影响细胞内部的 pH (pHi ) 。一个房间怎么对 pHi 变化作出回应维持 bioenergetic 动态平衡,留下逃犯。这里,我们显示出那 Smad5,发信号的形态基因的蛋白质(BMP ) 回答到 pHi 的骨头的一个描绘得好的下游的部件变化。冷、基本或张力亢进的条件增加 pHi,它接着在 Smad5 的 MH1 域以内声言不赞成控告的氨基酸簇,推动它到细胞质的从原子核的重定位。在另一方面,加热,酸或张力减退的条件减少 pHi,堵住 Smad5 的原子出口,并且因此引起它的原子累积。环境变化和 pHi 变化导致的 Smad5 的活跃 nucleocytoplasmic shuttling 独立于 BMP 发信号, carboxyl 终点 phosphorylation 和 Smad4。另外, Smad5 的脱离引起在区别识别了的神经发展过程人的 pluripotent 干细胞建模的细胞的 bioenergetic 动态平衡和破坏正常的长期、不可逆的 dysregulation。重要地,在 Smad5 缺乏的房间的这些新陈代谢、发展的赤字能仅仅由细胞质的 Smad5 被救。细胞质的 Smad5 身体上与 hexokinase 交往 1 并且加速 glycolysis。一起,我们的调查结果显示 Smad5 充当一个 pHi 送信人并且由调整细胞质的新陈代谢的机械维持房间的 bioenergetic 动态平衡。 | Yujiang Fang Zhongliang Liu Zhenyu Chen Xiangjie Xu Mengtao Xiao Yanyan Yu Yuanyuan Zhang Xiaobai Zhang Yanhua Du Cizhong Jiang Yuzheng Zhao Yiran Wang Beibei Fan Daniel Terheyden-Keighley Yang Liu Lei Shi Yi Hui Xin Zhang Bowen Zhang Hexi Feng Lin Ma Quanbin Zhang Guohua Jin Yi Yang Bin Xiang Ling Liu Xiaoqing Zhang | 2017 | Cell Research2017，27，9： | 2 |
| 10 | The effect of collagen hydrogels on chondrocyte behaviors through restricting the contraction of cell/hydrogel constructs显示文摘Collagen is a promising material for tissue engineering,but the poor mechanical properties of collagen hydrogels,which tend to cause contraction under the action of cellular activity,make its application challengeable.In this study,the amino group of type I collagen(Col I)was modified with methacrylic anhydride(MA)and the photo-crosslinkable methacrylate anhydride modified type I collagen(CM)with three different degrees of substitution(DS)was prepared.The physical properties of CM and Col I hydrogels were tested,including micromorphology,mechanical properties and degradation properties.The results showed that the storage modulus and degradation rate of hydrogels could be adjusted by changing the DS of CM.In vitro,chondrocytes were seeded into these four groups of hydrogels and subjected to fluorescein diacetate/propidium iodide(FDA/PI)staining,cell counting kit-8(CCK-8)test,histological staining and cartilage-related gene expression analysis.In vivo,these hydrogels encapsulating chondrocytes were implanted subcutaneously into nude mice,then histological staining and sulfated glycosaminoglycan(sGAG)/DNA assays were performed.The results demonstrated that contraction of hydrogels affected behaviors of chondrocytes,and CM hydrogels with suitable DS could resist contraction of hydrogels and promote the secretion of cartilage-specific matrix in vitro and in vivo. | Longpeng Dong Qingli Liu Yongli Gao Hengxing Jia Wenling Dai Likun Guo Hongsong Fan Yujiang Fan Xingdong Zhang | 2021 | Regenerative Biomaterials2021，8，4： | 2 |
| 11 | 显示文摘 | Yujiang Fan Haruo Nishida Yoshihito Shirai | 2004 | Poly- mer Degradation and Stability2004，84，1： | 1 |
| 12 | Optimal Crashworthiness Design of a Spot--welded Thin-- walled Hat Section显示文摘 | Xiang Yujiang Wang Qian Fan Zijie | 2006 | Finite Elements in Analysis and Design2006，42，2： | 1 |
| 13 | Thermal degradation of poly(L-lactide):Effect of alkali earth metal oxides for selective L,L-lactide formation显示文摘 | Yujiang Fan Haruo Nishida Tomokazu Mori | | 0，，4： | 1 |
| 14 | Optimal crashworthiness design of a spot-welded thin-walled hat section显示文摘 | XIANG Yujiang WANG Qian FAN Zijie | 2006 | Finite Elements in Analysis and Design2006，42，10： | 1 |
| 15 | Optimal crash worthiness design of a spot - welded thin - walled hat section显示文摘 | Yujiang XIANG Qian WANG Zijie FAN | 2006 | Finite Elements in Analysis and Design2006，42，10： | 1 |
| 16 | Optimal crash worthiness design of a spot-welded thin-walled hat section显示文摘 | Yujiang Xiang Qian Wang Zijie Fan | 2006 | Finite Elements in Analysis and Design2006，42，10： | 1 |
| 17 | Synthesis of photo-reactive poly(vinyl alcohol)and construction of scaffold-free cartilage like pellets in vitro显示文摘Photo-reactive poly(vinyl alcohol)(PRPVA)was synthesized by introduction of phenyl azido groups into poly(vinyl alcohol)(PVA)and applied for surface modification.PRPVA was grafted onto cell culture plate surface homogeneously or in a micropattern.Human mesenchymal stem cells(hMSCs)cultured on cell culture plate surface and PVA-modified surface showed different behaviors.Cells adhered and spread well on cell culture plate surface,while they did not adhere on PVA-grafted surface at all.When hMSCs were cultured on PVA-micropatterned surface,they formed a cell micropattern.Cells formed pellets after cultured on PVA homogeneously modified surface in chondrogenic induction medium for 2 weeks.The pellets were positively stained by hematoxylin/eosin,safranin-O/fast green and toluidin blue,and they were also stained brown by Type II collagen and proteoglycan immunohistological staining.Real-time PCR analysis was conducted to investigate the expression of colI,colII,colX,aggrecan and sox9 mRNA.Results of gene expression were in agreement with those of histological and immunohistological observations.These results indicated that hMSCs cultured on PVA-modified surface performed chondrogenic differentiation,and it was possible to construct scaffold-free cartilage like pellets with PVA-modified surface in vitro. | Bao Li Yongli Gao Likun Guo Yujiang Fan Naoki Kawazoe Hongsong Fan Xingdong Zhang Guoping Chen | 2018 | Regenerative Biomaterials2018，5，3： | 1 |
| 18 | Thermal degradation behaviour of poly(lactic acid) stereocomplex显示文摘 | Yujiang Fan Haruo Nishida Yoshihito Shirai | 2004 | Polymer Degradation and Stability2004，86，2： | 1 |
| 19 | Optimal crashworthiness design of a spot-welded thin-walled hat section显示文摘 | Xiang Yujiang Wang Qian Fan Zijie | 2006 | Finite Elements in Analysis and Design2006，42，10： | 1 |
| 20 | Thermal stability of poly (L iactide) : influence of end protection by acetyl group显示文摘 | Yujiang Fan Haruo Nishida Yoshihito Shirai | 2004 | Polymer Degradation and Stability2004，84，1： | 1 |
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