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| 1 | Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2显示文摘In contrast to the extensive research about viral protein–host protein interactions that has revealed major insights about how RNA viruses engage with host cells during infection,few studies have examined interactions between host factors and viral RNAs(vRNAs).Here,we profiled vRNA–host protein interactomes for three RNA virus pathogens(SARS-CoV-2,Zika,and Ebola viruses)using ChIRP-MS.Comparative interactome analyses discovered both common and virus-specific host responses and vRNA-associated proteins that variously promote or restrict viral infection.In particular,SARS-CoV-2 binds and hijacks the host factor IGF2BP1 to stabilize vRNA and augment viral translation.Our interactome-informed drug repurposing efforts identified several FDA-approved drugs(e.g.,Cepharanthine)as broad-spectrum antivirals in cells and hACE2 transgenic mice.A co-treatment comprising Cepharanthine and Trifluoperazine was highly potent against the newly emerged SARS-CoV-2 B.1.351 variant.Thus,our study illustrates the scientific and medical discovery utility of adopting a comparative vRNA-host protein interactome perspective. | Shaojun Zhang Wenze Huang Lili Ren Xiaohui Ju Mingli Gong Jian Rao Lei Sun Pan Li Qiang Ding Jianwei Wang Qiangfeng Cliff Zhang | 2022 | Cell Research2022，32，1： | 5 |
| 2 | Systematic analysis of clinical relevance and molecular characterization of m^(6)A in COVID-19 patients显示文摘N6-methyladenosine(m6A)is the most prevalent post-transcriptional RNA modification in mRNA and long non-coding RNAs of eukaryotes,and its biological functions are mediated by m6A writers,erasers and readers.1 A nuclear methyltransferase complex consisting of METTL3,METTL14,WTAP,VIRMA,ZC3H13,RBM15(or RBM15B),YWHAG,TRA2A and CAPRIN1 catalyzes the m6A modifications,acting as m6A writers.1 m6A demethylase ALKBH5 as well as m6A demethylase FTO mediate the demethylation of m6As,acting as the m6A erasers. | Sanqi An Zhouhua Xie Yanyan Liao Junjun Jiang Wenyi Dong Fuqiang Yin Wen-Xing Li Li Ye Jianyan Lin Hao Liang | 2022 | Genes & Diseases2022，9，5： | 1 |
| 3 | N^(6)-methyladenosine and Its Implications in Viruses显示文摘N6-methyladenine(m^(6)A)is the most abundant RNA modification in mammalian messenger RNAs(mRNAs),which participates in and regulates many important biological activities,such as tissue development and stem cell differentiation.Due to an improved understanding of m^(6)A,researchers have discovered that the biological function of m^(6)A can be linked to many stages of mRNA metabolism and that m^(6)A can regulate a variety of complex biological processes.In addition to its location on mammalian mRNAs,m^(6)A has been identified on viral transcripts.m^(6)A also plays important roles in the life cycle of many viruses and in viral replication in host cells.In this review,we briefly introduce the detection methods of m^(6)A,the m^(6)A-related proteins,and the functions of m^(6)A.We also summarize the effects of m^(6)A-related proteins on viral replication and infection.We hope that this review provides researchers with some insights for elucidating the complex mechanisms of the epitranscriptome related to viruses,and provides information for further study of the mechanisms of other modified nucleobases acting on processes such as viral replication.We also anticipate that this review can stimulate collaborative research from different fields,such as chemistry,biology,and medicine,and promote the development of antiviral drugs and vaccines. | Yafen Wang Xiang Zhou | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 4 | m^(6)A甲基化修饰在呼吸道病毒复制及宿主免疫应答中的研究进展显示文摘N^(6)-甲基腺苷修饰(N^(6)-methyladenosine,m^(6)A)是真核细胞mRNA最普遍的内部修饰,受多种m^(6)A修饰酶的动态调控,包括甲基转移酶、去甲基化酶和特异性结合蛋白。近年来呼吸道病毒感染受到广泛关注,病毒在宿主细胞中的复制代谢过程受到m^(6)A调节。本文就m^(6)A调控酶的作用机制,m^(6)A修饰在呼吸道病毒复制中的作用,包括腺病毒、甲型流感病毒、新型冠状病毒、呼吸道合胞病毒和人偏肺病毒,以及病毒m^(6)A修饰对宿主免疫应答的影响进行综述,为探究病毒表观转录组修饰的调控作用和抗病毒靶点研究或抗病毒疫苗研发提供参考。 | 刘佳程 高燕 | 2023 | 中华临床感染病杂志2023，16，2： | 0 |
| 5 | m6A甲基化修饰在人类病毒感染复制中的作用显示文摘真核细胞内RNA的m6A甲基化修饰通过调节基因的剪接、定位、稳定和翻译影响基因功能,其中甲基转移酶、去甲基化酶和阅读蛋白参与甲基化修饰过程。病毒感染引起传染性疾病,近年发现在病毒与宿主的博弈过程中,病毒RNA会受宿主m6A修饰系统的调控进而影响病毒复制,病毒蛋白通过影响宿主甲基化修饰相关调节因子从而影响整体修饰水平,改变细胞因子水平、干扰免疫细胞增殖和活性以及降低应激反应,以确保病毒顺利复制。本文概述了m6A甲基化修饰在甲型流感病毒(IAV)、人类I型免疫缺陷病毒(HIV-I)、新型冠状病毒(SARS-CoV-2)这三种典型的RNA病毒及乙型肝炎病毒(HBV)、Epstein-Barr病毒(EBV)、腺病毒(AdV)的DNA病毒感染复制中的作用。这些工作有助于探索靶向m6A调节因子的抑制剂,为开发新的抗病毒药物提供理论基础。 | 彭倩 乔嘉璐 李卫玲 孙宾莲 胡康洪 | 2023 | 中国病原生物学杂志2023，18，5： | 0 |
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