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| 1 | 中国非洲猪瘟研究进展显示文摘非洲猪瘟(African swine fever,ASF)是由非洲猪瘟病毒(African swine fever virus,ASFV)感染家猪和野猪引起的一种急性、烈性和高度接触性传染病,可以造成严重经济损失和威胁全球养猪业的发展.根据世界动物卫生组织(World Organisation for Animal Health,WOAH)报告,ASF于1921年在肯尼亚被首次报道,已经在非洲、亚洲、美洲、欧洲和大洋洲等地区暴发,严重危害全球养猪业的发展.ASF至今已有上百年历史,目前仍未有可供全球推广使用商业化的疫苗和抗病毒药物;ASFV基因功能、致病机制、免疫保护、免疫逃逸等关键科学问题依然未知.2018年,ASF传入中国,给中国生猪产业造成严重影响.面向ASF防控和科技攻关重大需求,中国农业科学院、中国科学院、清华大学等多家科研机构和企业都紧急建立科研队伍,对ASFV开展了科技攻关,并取得了丰硕的成果.其中,中国农业科学院哈尔滨兽医研究所第一时间分离鉴定出第一株ASFV毒株、基因Ⅱ型自然变异株、基因Ⅰ型病毒株、基因Ⅰ型/Ⅱ型自然重组病毒株,并完成其基因组遗传进化和致病特性研究;联合解析了ASFV结构和多种病毒基因功能;成功研制了ASF基因缺失弱毒活疫苗等多种防控产品,为中国ASFV的认知及防控做出重要贡献.本文围绕过去五年来中国在ASFV流行病学、病原学、病毒结构生物学、毒力功能基因挖掘、疫苗创制及诊断产品研发等方面的研究进展进行综述,以期为中国非洲猪瘟防控与研究提供参考. | 张振江 孙恩成 朱远茂 李芳 步志高 赵东明 | 2023 | 中国科学:生命科学2023，53，12： | 1 |
| 2 | Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies显示文摘African swine fever(ASF)continues to cause enormous economic loss to the global pig industry.Since there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control measures.Indirect immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody detection.To standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum samples.The IFA reacted with the ASFV-positive sera and displayed bright fluorescence foci.There was no non-specific green fluorescence due to cellular senescence or other cell damage-causing factors.Compared to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our IFA.The detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than iELISA.The newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF. | Wan Wang Zhenjiang Zhang Weldu Tesfagaber Jiwen Zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu Yuanmao Zhu Dongming Zhao | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
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