|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control. | Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu | 2021 | Science China(Life Sciences)2021，64，5： | 46 |
| 2 | Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum显示文摘African swine fever(ASF)is a highly infectious,transboundary viral disease of domestic and wild pigs,and is currently the most serious threat to world swine production,resulting in significant economic loss.In the absence of vaccines and treatments,the control of the disease entirely depends on accurate and early diagnosis accompanied by the culling of infected pigs.Thus,a highly specific and sensitive diagnostic assay is required during an outbreak and surveillance of the disease.In this study,a highly sensitive,specific,rapid and repeatable P22-monoclonal antibody-based blocking enzyme-linked immunosorbent assay(bELISA)assay was developed for the detection of antibodies against genotype I and II African swine fever viruses(ASFVs).A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic assay.To determine the PI(percent Inhibition)cut-off value,receiver-operating characteristic(ROC)analysis was applied.According to the ROC analysis of the data,98.10%specificity and 100%sensitivity were recorded when the threshold cut-off value of PI was established at 47%.In addition,the assay was able to detect ASFV antibodies as early as 9 days post-infection when serum samples from experimentally infected pigs were used.Taking all together,the results of the present study indicated that the P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV,which could play a valuable role in the containment and prevention of ASFV as an alternative to other serological diagnostic methods.Also,this study will assist researchers to further investigate the immunogenic importance of P22 protein in ASFV infection. | Ghebremedhin Tsegay Weldu Tesfagaber Yuanmao Zhu Xijun He Wan Wang Zhenjiang Zhang Encheng Sun Jinya Zhang Yuntao Guan Fang Li Renqiang Liu Zhigao Bu Dongming Zhao | 2022 | Biosafety and Health2022，4，4： | 2 |
| 3 | Pulse control of frequency and width for a real-time independently adjustable laser source显示文摘A set of semiconductor laser pulse seed sources based on an embedded chip is proposed.The greatest feature is that the optical pulse frequency and width can be independently adjusted in real time.The pulse seed sources can be switched independently and online from the gain-switched mode to the quasi-continuous wave mode to obtain optimal optical parameters for specific applications.To explore the physical mechanism of the semiconductor laser source,the rate equation that describes the carrier-photon transient change in a semiconductor laser cavity is numerically derived and solved.Subsequently,problems that need to be considered while designing the drive circuit are identified.The system evaluation indicates that the optical pulse frequency adjustment range is 250 Hz to 42 MHz,and the narrowest optical pulse output width is 80 ps.The pulse seed source can drive semiconductor lasers with different central wavelengths(1064,1550,and 1970 nm),and can also simultaneously drive two semiconductor lasers and output dual-band optical pulses.It can be used as a seed source for general high-power optical systems,and exhibits good application value and extensive market prospects. | Zhiwei YANG Xu WU Deqin OUYANG Encheng ZHANG Huibin SUN Shuangchen RUAN | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，10： | 0 |
| 4 | Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies显示文摘African swine fever(ASF)continues to cause enormous economic loss to the global pig industry.Since there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control measures.Indirect immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody detection.To standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum samples.The IFA reacted with the ASFV-positive sera and displayed bright fluorescence foci.There was no non-specific green fluorescence due to cellular senescence or other cell damage-causing factors.Compared to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our IFA.The detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than iELISA.The newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF. | Wan Wang Zhenjiang Zhang Weldu Tesfagaber Jiwen Zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu Yuanmao Zhu Dongming Zhao | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
| 5 | Distinct but correct:generating diversified and entity-revised medical response显示文摘Medical dialogue generation(MDG)is applied for building medical dialogue systems for intelligent consultation.Such systems can communicate with patients in real time,thereby improving the efficiency of clinical diagnosis.However,predicting correct entities and correctly generating distinct responses remain a great challenge.Inspired by actual doctors'responses to patients,we consider MDG a two-stage task:entity prediction and dialogue generation.For entity prediction,we design an ent-mac post pre-training strategy by leveraging external medical entity knowledge to enhance the pre-trained model.For dialogue generation,we propose an entity-aware fusion MDG method in which predicted entities are integrated into the dialogue generation model through different encoding fusion mechanisms,using information from different sources.Because the diverse beam search algorithm can produce responses with entities that deviate from the predicted entities,an entity-revised diverse beam search is proposed to correct the entities entailed in the generated responses and make the generated responses more distinct.The experimental results on the China Conference on Knowledge Graph and Semantic Computing 2021(A/B tests)and the International Conference on Learning Representations 2021(online test)datasets show that the proposed method outperforms several state-of-the-art methods,which demonstrates its practicability and effectiveness. | Bin LI Bin SUN Shutao LI Encheng CHEN Hongru LIU Yixuan WENG Yongping BAI Meiling HU | 2024 | Science China(Information Sciences)2024，67，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员