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| 1 | Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway显示文摘Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation. | ZHANG JinXiang1, WANG Hui2, XIAO Qing3, LIANG HuiFang4, LI ZhuoYa4, JIANG ChunFang1, WU HeShui5 & ZHENG QiChang5 1 Department of Emergency Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Medical Genetics, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 3 Department of Ophthalmology, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 4 Department of Medical Immunology, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 5 Department of General Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China | 2009 | Science China(Life Sciences)2009，52，2： | 33 |
| 2 | Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment:a radioimmunoconjugate for hepatocellular carcinoma imaging显示文摘AIM To directly radiolabel an anti-hepatomamAb fragment HAb18 F(ab’)2 with 99mTc bystannous-reduced method,and assess thestability,biodistribution and radioimmun-oimaging(RⅡ).METHODS Immunoreactive fraction wasdetermined according to Lindmo’s method.Ellman’s reagent was used to determine thenumber of thiols in the reduced F(ab’)2.Labelingefficiency and homogeneity were measured bypaper chromatography,sodium dodecylsulphatepolyacrylamide gel electrophoresis(SDS-PAGE)and autoradiography.Challenge assay involvedthe incubation of aliquots of labeled antibody inethylenediaminetetraacetate( EDTA )and L-cysteine(L-cys)solutions with different molarratio at 37℃ for 1h,respectively.Investigationsin vivo utilized nude mice bearing humanhepatocellular carcinoma(HHCC)xenograftswith gamma camera imaging and tissuebiodistribution studies at regular intervals.RESULTS The labeling procedure was finishedwithin 1.5 h compared with the'pretinning'method which would take at least 21h.In vitrostudies demonstrated that the radiolabeled mAbfragment was homogeneous and retained itsimmunoreactivity.Challenge studies indicatedthat 99mTc-labeled HAb18 F(ab’)2 in EDTA is morestable than in L-cys.Imaging and biodistribution showed a significant tumor uptake at 24 h post-injection of 99mTc-labeled HAb18 F(ab’)2.Theblood,kidney,liver and tumor uptakes at 24hwere 0.56±0.09,56.45±11.36,1.43±0.27 and6.57±3.01(%ID/g),respectively.CONCLUSION 99mTc-HAb18 F(ab’)2 conjugateprepared by this direct method appears to be aneffective way to detect hepatoma in nude micemodel. | Hui Jie Bian Zhi Nan Chen Jing Lan Deng | 2000 | World Journal of Gastroenterology2000，6，3： | 13 |
| 3 | ^(99m)Tc-labeled HAb18 McAb Fab fragment for radioimmunoimaging in nude mice bearing human hepatocellular carcinoma显示文摘99mTclabeledHAb18McAbFabfragmentforradioimmunoimaginginnudemicebearinghumanhepatocelularcarcinomaQIUKai1,2,WANGBoChen1,CHE... | Qiu K Wang BC Chen ZN Fang P Liu CG Wan WX Liu YF | 1998 | World Journal of Gastroenterology1998，4，2： | 8 |
| 4 | 不同金银花种质资源ISSR遗传多样性研究显示文摘应用ISSR标记技术,对来自国内忍冬属(Lonicera Linn.)的19个居群进行遗传多样性和遗传结构的分析.从100条ISSR随机引物中筛选出19条引物对19个忍冬居群进行扩增,共得到305条清晰的扩增位点,其中多态性位点273个,多态位点百分率(PPB)为89.51%.遗传分化系数(G_(sr))为0.5624,这显示19个居群的分子变异主要存在于居群间. | 董桂灵 胡尚钦 陈晓敏 黄璐琳 杨晓 童文 白林含 | 2009 | 四川大学学报(自然科学版)2009，46，6： | 7 |
| 5 | Pathological significance of tRNA-derived small RNAs in neurological disorders显示文摘Non-coding RNAs(ncRNAs) are a type of RNA that is not translated into proteins. Transfer RNAs(tRNAs), a type of ncRNA, are the second most abundant type of RNA in cells. Recent studies have shown that tRNAs can be cleaved into a heterogeneous population of ncRNAs with lengths of 18–40 nucleotides, known as tRNA-derived small RNAs(tsRNAs). There are two main types of tsRNA, based on their length and the number of cleavage sites that they contain: tRNA-derived fragments and tRNA-derived stress-induced RNAs. These RNA species were first considered to be byproducts of tRNA random cleavage. However, mounting evidence has demonstrated their critical functional roles as regulatory factors in the pathophysiological processes of various diseases, including neurological diseases. However, the underlying mechanisms by which tsRNAs affect specific cellular processes are largely unknown. Therefore, this study comprehensively summarizes the following points:(1) The biogenetics of tsRNA, including their discovery, classification, formation, and the roles of key enzymes.(2) The main biological functions of tsRNA, including its miRNA-like roles in gene expression regulation, protein translation regulation, regulation of various cellular activities, immune mediation, and response to stress.(3) The potential mechanisms of pathophysiological changes in neurological diseases that are regulated by tsRNA, including neurodegeneration and neurotrauma.(4) The identification of the functional diversity of tsRNA may provide valuable information regarding the physiological and pathophysiological mechanisms of neurological disorders, thus providing a new reference for the clinical treatment of neurological diseases. Research into tsRNAs in neurological diseases also has the following challenges: potential function and mechanism studies, how to accurately quantify expression, and the exact relationship between tsRNA and miRNA. These challenges require future research efforts. | Chuan Qin Pei-Pei Xu Xin Zhang Chao Zhang Chang-Bin Liu De-Gang Yang Feng Gao Ming-Liang Yang Liang-Jie Du Jian-Jun Li | 2020 | Neural Regeneration Research2020，15，2： | 5 |
| 6 | Use of chondral fragments for one stage cartilage repair: A systematic review显示文摘AIM: To investigate the state of the art regarding Cartilage Autograft Implantation System(CAIS) or Particulated Juvenile Allograft Cartilage(PJAC).METHODS: The authors searched the English literature regarding CAIS and PJAC. The search strategy was:(particulated cartilage) OR autologous cartilage fragments. All basic science articles were included. Clinical articles with less than 10 patients treated and less than 6 mo of follow-up were excluded. With these criteria, a total of 17 articles were available for the present review. RESULTS: PJAC and CAIS are relatively novel techniques for cartilage repair. Good basic science evidence was described to support the concept. Although the preliminary clinical reports show encouraging results, clinical data are still limited, especially for CAIS. The indications for both techniques need to be precisely defined(age of the patients, size of the lesion, and involvement of the subchondral bone), together with other debated issues. CONCLUSION: In conclusion, the authors can state that encouraging preliminary results are available for both techniques. However, further studies are necessary to precisely determine the indications, surgical techniques, and long term outcomes for PJAC and CAIS. | Davide Edoardo Bonasia Antongiulio Marmotti Federica Rosso Gianluca Collo Roberto Rossi | 2015 | World Journal of Orthopedics2015，6，11： | 5 |
| 7 | Concurrent removal of intravitreal lens fragments after phacoemulsification with pars plana vitrectomy prevents development of retinal detachment显示文摘AIM: To evaluate the outcomes of 'concurrent vitrectomy' to retrieve dislocated lens fragment during phacoemulsification.METHODS: In a retrospective, observational case series, data of patients who underwent 'concurrent' pars plana vitrectomy(PPV) for dislocated lens fragments between the period 2000 and 2008 were reviewed. Data collected included patient demographics, pre-operative visual acuity, intra-operative occurrence of retinal breaks, duration of follow up, post-operative intraocular pressure, final best-corrected visual acuity(BCVA),presence of cystoid macular edema(CME) and occurrence of rhegmatogenous retinal detachment(RRD).· RESULTS: A total of 58 eyes of 58 patients were included in the study. At 12 mo the mean postoperative BCVA was log MAR 0.17(20/30) with a range of log MAR0 to 0.69(20/20 to 20/100), with 96.6%(56/58) of patients showing post- operative improvement in visual acuity(P =0.005). None of the patients developed postoperative retinal detachment, endophthalmitis or non-resolving uveitis at 12 mo.CONCLUSION: Our study results suggest concurrent PPV for retained lens fragments after cataract surgery is beneficial and may decrease the risk of glaucoma and prevent development of RRD. | Kakarla V Chalam Ravi K Murthy Joshua C Priluck Vijay Khetpal Shailesh K Gupta | 2015 | International Journal of Ophthalmology(English edition)2015，8，1： | 4 |
| 8 | Projected area and drag coefficient of high velocity irregular fragments that rotate or tumble显示文摘3 degrees of freedom(DOF)exterior ballistic computer models are used in fragment studies to calculate individual trajectories of each fragment based on drag coefficient and the projected(presented)area in the direction of velocity of center of mass.The expectation of a randomly distributed projected area is commonly used for fragments that tumble(random rotation)during flight.We forecast a model where the expected drag coefficient is dependent of shape and Mach number.Rotation or tumbling only affects the expected projected area.Models of projected areas during tumbling and rotation are presented.An examination of the data by Mc Cleskey(1988)indicates that the volume of the fragment to the power of2/3 is a better parameter to characterize the drag coefficient of the fragments than the maximum projected area.Hydrocode simulations are used to verify results and to study projected area and drag coefficient of fragments. | John F. Moxnes 0yvind Fr0 yland Ivar J. 0ye Tom I. Brate Eva Friis Gard 0degardstuen Tallak H. Risdal | 2017 | Defence Technology(防务技术)2017，13，4： | 3 |
| 9 | A novel method for determination of lethal radius for high-explosive artillery projectiles显示文摘A new model has been defined that enables the estimation of the lethal radius(radius of efficiency)of HE(High Explosive)artillery projectiles against human targets.The model is made of several modules:CAD(Computer Aided Design)modeling,fragment mass distribution estimation,fragment initial velocity prediction,fragment trajectory calculation,effective fragment density estimation,and high explosive projectile lethal radius estimation.The results were compared with the experimental results obtained based on tests in the arena used in our country,and the agreement of the results was good.This model can be used in any terminal-ballistics scenario for high explosive projectiles since it is general,parametric,fast and relatively easy to implement. | Alan Catovic Elvedin Kljuno | 2021 | Defence Technology(防务技术)2021，17，4： | 2 |
| 10 | Product yields for the photofission of ^(232)Th,^(234,238)U,^(237)Np,and ^(239,240,242)Pu actinides at various incident photon energies显示文摘Photofission fragments mass yield for^(232)Th,^(234;238) U,^(237) Np, and^(239;240;242) Pu isotopes are investigated.The calculations are done using a developed approach based on Gorodisskiy's phenomenological formalism. The Gorodisskiy's method is developed to be applied for the neutron-induced fission. Here we revised it for application to photofission. The effect of emitted neutron prior to fission on the fission fragment mass yields has also been studied. The peak-to-valley ratio is extracted for the240 Pu isotope as a function of energy. Obtained results of the present formalism are compared with the available experimental data. Satisfactory agreement is achieved between the results of present approach and the experimental data. | M.R.Pahlavani P.Mehdipour | 2018 | Nuclear Science and Techniques2018，29，10： | 2 |
| 11 | 别样的保护与传承——梁雷《色拉西片断》创作始末与音乐分析显示文摘旅美作曲家梁雷有着浓厚的蒙古族音乐情结。这种情结不仅体现在他对蒙古族音乐的搜集与保护上,还体现在他采用蒙古族音乐素材创作的作品中。弦乐四重奏《色拉西片断》就是为纪念蒙古族潮尔大师色拉西(Serashi,1887~1968)而作。这部作品凝结了作曲家对蒙古族音乐的深刻理解与别样传承。 | 班丽霞 | 2008 | 黄钟(武汉音乐学院学报)2008，，3： | 2 |
| 12 | Underwater explosion effects of 60 mm H.E. mortar bomb on a cylindrical concrete structure-PIT显示文摘PIT tests are usually performed when a mass distribution of High Explosive(H.E) projectile fragments is required. This paper shows the underwater detonation effects of 60 mm, M90 H.E. mortar bomb filled with Comp. B on cylindrical concrete structure(concrete pipe closed at one end-similar to a PIT test)which is 2 m high(inner height) with inner diameter of also 2 m. Thickness of both wall and bottom of a pipe is 0.35 m. Detailed characteristics of concrete which is used for manufacturing of a pipe are specified. Mortar bomb is submerged directly in to the water(no free airspace around the bomb) with the nose pointing to the bottom of a pipe. Number and mass of fragments after detonation are presented by table and photographs. Fragments of dummy fuze, through which blasting cap was protruded, are collected and reassembled to form a shape of a fuze after detonation where expanding of fuze material due to a detonation products is visualized. After underwater detonation, detonation of the same mortar bomb is performed in an empty pipe and the effects of this kind of detonation are observed. Distance at which fragments generated from submerged mortar bomb will not reach concrete pipes wall is also determined. | Faruk Razic Burek Miralem | 2019 | Defence Technology(防务技术)2019，15，1： | 2 |
| 13 | Impact of patch size on woody tree species richness and abundance in a tropical montane evergreen forest patches of south India显示文摘We examined the effects of forest patch size on woody tree species richness and abundance in tropical montane evergreen forest patches of the Nilgiri region,south India. We sampled woody trees(>1 cm dbh) from 21 forest patches in the upper Nilgiri hills(> 2000 m elevation) and recorded a total of 35,146 individuals of 61 species, 45 genera and 30 families. Species richness and abundance of sapling/shrubs(≥ 1 to < 10 cm dbh)increased significantly with increasing patch size, but the species richness and abundance of small, medium and larger trees(≥ 10 to < 30, ≥ 30 to < 60 and ≥ 60 cm dbh,respectively) did not. Overall, forest interior species richness and abundance increased significantly with increasing patch size but edge species richness did not. Species richness and abundance of shade-tolerant and shade-demanding tree species also increased with increasing patch size. The abundance of zoochory dispersed tree species was significantly related to increasing patch size, but those dispersed by autochory did not display any clear relationship between patch size and species richness or abundance.Our findings suggest that with increasing forest patch area,tree compositional patterns may be driven by species specific shade-tolerance adaptations and dispersal patterns.Differential responses in these traits by the plant community within the individual habitat zones of forest edge and interiors likely plays a major role in determining the inherent plant community and thus the subsequent ecological processes of forest patches, including their responses to increasing patch area. | Dharmalingam Mohandass Mason J.Campbell Priya Davidar | 2018 | Journal of Forestry Research2018，29，6： | 1 |
| 14 | Erratum to: Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The same figure was misused for the PCR/RE assay results of Gn1a and GW2 fragments in Figure 3,and the arrows in the graphicsal result of GW2 were not on the tape.The corrected Figure 3 is as follows. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2019 | Science China(Life Sciences)2019，62，9： | 1 |
| 15 | A generalized model for estimation of aerodynamic forces and moments for irregularly shaped bodies显示文摘A novel method for estimation of an aerodynamic force and moment acting on an irregularly shaped body (such as HE projectile fragments) during its flight through the atmosphere is presented. The model assumes that fragments can be approximated with a tri-axial ellipsoid that has continuous surface given as a mathematical function. The model was validated with CFD data for a tri-axial ellipsoid and verified using CFD data on aerodynamic forces and moments acting on an irregularly shaped fragment. The contribution of this method is that it represents a significant step toward a modeling that does not require a cumbersome CFD simulation results for estimation of fragment dynamic and kinematic parameters. Due to this advantage, the model can predict the fragment motion consuming a negligible time when compared to the corresponding time consumed by CFD simulations. Parametric representation (generalization) of the fragment geometrical data and the conditions provides the way to analyze various correlations and how parameters influence the dynamics of the fragment flight. | Elvedin Kljuno Alan Catovic | 2019 | Defence Technology(防务技术)2019，15，3： | 1 |
| 16 | 骨桥蛋白中多肽片段的FT-ICR-MS分析显示文摘Biological mass spectrometry has received a great progress in recent years.The osteopontin(OPN) isolated and purified from the human milk was studied by HPLC using SCX and C4 columns.After hydrolyzing the purified OPN sample by trypsin,the correlative polypeptide fragments GDSVVYGLR and QNLLAPQTLPSK were obtained by FT-ICR-MS.It showed that the method of nano-spray HPLC combined with FT-ICR-MS and Mascot search was very efficient for the analysis of the purified OPN and its polypeptide fragments.Further study provides more academic theories of their different modifications and the relative bioactivities. | 石磊 王韶 刘晓梅 程舸 于雷 段芳芳 韩丽华 | 2008 | 分析测试学报2008，27，S1： | 1 |
| 17 | 麻疯树核糖体失活蛋白curcin基因的原核表达及其抗真菌活性的研究显示文摘通过PCR方法,将麻疯树核糖体失活蛋白curcin的开放阅读框片段从麻疯树总DNA中扩增出来.并将该克隆基因片段连接到原核表达载体pQE-30中,成功构建了重组质粒pQE-C,转化大肠杆菌M15菌株.经IPTG诱导后,curcin重组蛋白在大肠杆菌中成功表达.然后通过纯化原核表达产物,得到纯化的curcin重组蛋白,并以纸片法进行体外抗菌活性检测,发现其具有较强抗真菌活性. | 邵彩霞 秦小波 蔡峰 辜小平 闫毅武 王成世 徐莺 陈放 Cai-Xia Xiao-Bo Xiao-Ping Yi-Wu Cheng-Shi | 2009 | 四川大学学报(自然科学版)2009，46，6： | 1 |
| 18 | Estimation of projected surface area of irregularly shaped fragments显示文摘The essence and the main contribution of this paper are consisted of the suggested novel method for estimation of a projected surface area of an irregularly shaped fragment, which represents a significant step toward a new method of an aerodynamic force estimation of a fragment motion through a resistive medium. The suggested method is to use a tri-axial ellipsoid that has a continuous surface(given as a mathematical function) to approximate an irregularly shaped fragment so that the fragment trajectory can be estimated faster taking into consideration that the aerodynamic force is proportional to a projected surface area of the fragment.During their motion, fragments can take any orientation relative to the velocity vector and aerodynamic force is proportional to a projected surface area of fragment perpendicular to the velocity vector,so it is necessary to have a method for correct estimation of the projected area in an arbitrary direction relatively to the fragment.The model was validated with analytical and CAD techniques, and verified using 3D model of a real fragment. The comparison of the projected surface area values for the fragment, obtained using the CAD tools and using our model, are generally in good agreement, This method represents a step toward modeling that does not require a CFD result for estimation of the aerodynamic force of irregularly shaped bodies. | Elvedin Kljuno Alan Catovic | 2019 | Defence Technology(防务技术)2019，15，2： | 1 |
| 19 | Material Equivalent Test Based on Dimensional Analysis for Fragment Penetrating Antenna Target显示文摘Phased array radar is the main sensor in a battlefield.Phased array antenna is the main execution unit of the phased array radar,and it greatly affects the reliability of the phased array radar. As a result,the fragment damaged antenna test is important.As the materials of phased array antenna are not easy to get,the fragment damaged antenna test is difficult to carry out. Then we present a study on this problem and introduce the principles of dimensional analysis to solve it. Firstly, the fragments damage antenna target dimensionless model is constructed. Secondly,the finite element analysis software ANSYS / LS-DYNA are used to carry out a large number of different materials simulation test for dimensional analysis. Finally,based on dimensional test analysis,the materials equivalent empirical model between different antennas target is presented in the same damage. The results of this study provide a feasible and valuable solution for different materials' target damaged test. | 刘锋 石全 陈材 | 2016 | Journal of Donghua University(English Edition)2016，33，2： | 1 |
| 20 | Crustal structure and sedimentation history over the Alleppey platform, southwest continental margin of India:Constraints from multichannel seismic and gravity data显示文摘The Alleppey Platform is an important morphological feature located in the Kerala-Konkan basin off the southwest coast of India. In the present study, seismic reflection data available in the basin were used to understand the sedimentation history and also to carry out integrated gravity interpretation. Detailed seismic reflection data in the basin reveals that:(1) the Alleppey Platform is associated with a basement high in the west of its present-day geometry(as observed in the time-structure map of the Trap Top(K/T boundary)),(2) the platform subsequently started developing during the Eocene period and attained the present geometry by the Miocene and,(3) both the Alleppey platform and the Vishnu fracture zone have had significant impact on the sedimentation patterns(as shown by the time-structure and the isochron maps of the major sedimentary horizons in the region). The 3-D sediment gravity effect computed from the sedimentary layer geometry was used to construct the crustal Bouguer anomaly map of the region.The 3-D gravity inversion of crustal Bouguer anomaly exhibits a Moho depression below the western border of the platform and a minor rise towards the east which then deepens again below the Indian shield. The 2-D gravity modelling across the Alleppey platform reveals the geometry of crustal extension,in which there are patches of thin and thick crust. The Vishnu Fracture Zone appears as a crustal-scale feature at the western boundary of the Alleppey platform. Based on the gravity model and the seismic reflection data, we suggest that the basement high to the west of the present day Alleppey platform remained as a piece of continental block very close to the mainland with the intervening depression filling up with sediments during the rifting. In order to place the Alleppey platform in the overall perspective of tectonic evolution of the Kerala-Konkan basin, we propose its candidature as a continental fragment. | P.Unnikrishnan M.Radhakrishna G.K.Prasad | 2018 | Geoscience Frontiers2018，9，2： | 1 |
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