|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Diversity of Saccharomyces boulardii CNCM I-745 mechanisms of action against intestinal infections显示文摘The yeast Saccharomyces boulardii CNCM I-745 is one of the probiotics recommended for the prevention of antibiotic-associated diarrhea. Studies conducted in vivo and in vitro demonstrated that in the case of infectious diseases there are two potential sites of action of Saccharomyces boulardii CNCM I-745:(1)An action on enteropathogenic microorganisms(adhesion of bacteria and their elimination or an effect on their virulence factors: Toxins, lipopolysaccharide,etc.); and(2) a direct action on the intestinal mucosa(trophic effects, effects on epithelial reconstitution, anti-secretory effects, anti-inflammatory,immunomodulators). Oral administration of Saccharomyces boulardii CNCM I-745 to healthy subjects does not alter their microbiota. However, in the case of diseases associated with the use of antibiotics or chronic diarrhea, Saccharomyces boulardii CNCM I-745 can restore the intestinal microbiota faster. The interaction of Saccharomyces boulardii CNCM I-745 with the innate immune system have been recently demonstrated thus opening up a new therapeutic potential of this yeast in the case of diseases associated with intestinal infections but also other pathologies associated with dysbiosis such as inflammatory diseases. | Dorota Czerucka Patrick Rampall | 2019 | World Journal of Gastroenterology2019，25，18： | 18 |
| 2 | Effects of live yeast on differential genetic and functional attributes of rumen microbiota in beef cattle显示文摘Several studies have evaluated the effects of live yeast supplementation on rumen microbial population;however,its effect on differential microbial genes and their functional potential has not been described. Thus, this study applied shotgun metagenomic sequencing to evaluate the effects of live yeast supplementation on genetic and functional potential of the rumen microbiota in beef cattle. Eight rumen-cannulated Holstein steers were randomly assigned to two treatments in a cross-over design with two 25-day experimental periods and a 10-day wash-out between the two periods. The steers were housed in individual pens and fed 50% concentrate-mix and 50% red clover/orchard hay ad libitum. Treatments were(1) control(CON;basal diet without additive) and(2) yeast(YEA;basal diet plus 15 g/d of live yeast product). Rumen fluid samples were collected at 3, 6, and 9 h after feeding on the last d of each period. Sequencing was done on an Illumina Hi Seq 2500 platform. Dietary yeast supplementation increased the relative abundance of carbohydrate-fermenting bacteria(such as Ruminococcus albus, R.champanellensis, R. bromii, and R. obeum) and lactate-utilizing bacteria(such as Megasphaera elsdenii, Desulfovibrio desulfuricans, and D. vulgaris). A total of 154 differentially abundant genes(DEGs) were obtained(false discovery rate < 0.01). Kyoto Encyclopedia of Genes and Genomes(KEGG) annotation analysis of the DEGs revealed that 10 pathways, including amino sugar and nucleotide sugar metabolism, oxidative phosphorylation, lipopolysaccharide biosynthesis, pantothenate and coenzyme A biosynthesis, glutathione metabolism, beta-alanine metabolism,polyketide sugar unit biosynthesis, protein export, ribosome, and bacterial secretory system, were enriched in steers fed YEA. Annotation analysis of the DEGs in the carbohydrate-active enzymes(CAZy) database revealed that the abundance of genes coding for enzymes belonging to glycoside hydrolases, glycosyltransferases, and carbohydrate binding modules were enriched in steers fed YEA. These results confirm the effectiveness of a live S. cerevisiae product for improving rumen function in beef steers by increasing the abundance of cellulolytic bacteria, lactic acid-utilizing bacteria, and carbohydrate-active enzymes in the rumen. | Ibukun M.Ogunade Jerusha Lay Kenneth Andries Christina J.Mc Manus Frederick Bebe | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 11 |
| 3 | Production of β-1,3-glucanase and chitinase of two biocon-trol agents and their possible modes of action显示文摘Pichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3-glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3-glucanase activity was detected in Lilly-Barnett minimal salt medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3-glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2 SU. The lowest β-1,3-glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3-glucanase and chitinase activity was also triggered by wounding, adding of carbon sources | FAN Qing, TIAN Shiping, LIU Haibo & XU YongInstitute of Botany, Chinese Academy of Sciences, Beijing 100093, China | 2002 | Chinese Science Bulletin2002，47，4： | 11 |
| 4 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 5 | MdMYB6 regulates anthocyanin formation in apple both through direct inhibition of the biosynthesis pathway and through substrate removal显示文摘Anthocyanin biosynthesis and sugar metabolism are important processes during plant growth,but the molecular interactions underlying these pathways are still unclear.In this work,we analyzed the anthocyanin and soluble sugar contents,as well as the transcript levels of transcription factors that are known to be related to the biosynthesis of anthocyanin in‘Hongcui 1’apple flesh during fruit development.Overexpression of MdMYB6 in red-fleshed calli was found to reduce anthocyanin content and result in downregulated expression of the MdANS and MdGSTF12 proteins.Yeast one-hybrid and electrophoretic mobility shift analyses showed that MdMYB6 could directly bind to the promoters of MdANS and MdGSTF12,indicating that MdMYB6 could inhibit anthocyanin biosynthesis by regulating MdANS and MdGSTF12.Overexpression of MdTMT1 in the Arabidopsis tmt1 mutant restored the glucose and fructose contents to the wild-type levels,while overexpression of MdTMT1 in red-fleshed calli increased the contents of glucose and fructose but reduced the contents of UDP-glucose,UDP-galactose,and anthocyanin.Using a GUS reporter system,yeast one-hybrid,chromatin immunoprecipitation-PCR and electrophoretic mobility shift analyses,we found that MdMYB6 could bind to the promoter of MdTMT1,resulting in increased promoter activity.Overexpression of MdMYB6 in calli overexpressing MdTMT1 increased the expression of MdTMT1,which led to reduced contents of UDP-glucose and UDP-galactose and decreased anthocyanin content compared to those of the calli that overexpressed MdTMT1.This finding suggested that MdMYB6 could also inhibit anthocyanin biosynthesis by regulating MdTMT1 to decrease the contents of UDP-glucose and UDP-galactose.Taken together,these results showed that MdMYB6 and MdTMT1 play key roles in both anthocyanin biosynthesis and sugar transport. | Haifeng Xu Qi Zou Guanxian Yang Shenghui Jiang Hongcheng Fang Yicheng Wang Jing Zhang Zongying Zhang Nan Wang Xuesen Chen | 2020 | Horticulture Research2020，7，1： | 6 |
| 6 | Potential protective effects of red yeast rice in endothelial function against atherosclerotic cardiovascular disease显示文摘Atherosclerotic cardiovascular disease(ASCVD) is the deadliest disease in the world, with endothelial injury occurring throughout the course of the disease. Therefore, improvement in endothelial function is of essential importance in the prevention of ASCVD. Red yeast rice(RYR), a healthy traditional Chinese food, has a lipid modulation function and also plays a vital role in the improvement of endothelial reactivity and cardiovascular protection; thus, it is significant in the prevention and treatment of ASCVD. This article reviews the molecular mechanisms of RYR and its related products in the improvement of endothelial function in terms of endothelial reactivity, anti-apoptosis of endothelial progenitor cells, oxidative stress alleviation and anti-inflammation. | FENG Shu-Jun TANG Zhi-Han WANG Ying TANG Xin-Ying LI Tao-Hua TANG Wei KUANG Ze-Min | 2019 | Chinese Journal of Natural Medicines2019，17，1： | 6 |
| 7 | Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe显示文摘The metabolic enzyme CTP synthase(CTPS) is able to compartmentalize into filaments,termed cytoophidia,in a variety of organisms including bacteria,budding yeast,fission yeast,fruit flies and mammals.A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift.Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe.To our surprise,we find that both the length and the occurrence of cytoophidia in S.pombe decrease upon cold shock or heat shock.The temperature-dependent changes of cytoophidia are fast and reversible.Taking advantage of yeast genetics,we demonstrate that heat-shock proteins are required for cytoophidium assembly in S.pombe.Temperature sensitivity of cytoophidia makes S.pombe an attractive model system for future investigations of this novel membraneless organelle. | Jing Zhang Ji-Long Liu | 2019 | Journal of Genetics and Genomics2019，46，9： | 5 |
| 8 | CERS2基因在浸润性乳腺癌组织中的表达及其意义显示文摘CERS2 (ceramide synthase 2)又称LASS2(homo sapiens Iongevity assurance ohomologue 2 of yeast LAG1)。对CERS2基因的研究主要集中在肝癌,它不仅可抑制肝癌细胞的增殖,还可抑制其转移。 | 王艳艳 高立勇 赵跃华 李金运 罗琴 樊少华 | 2013 | 中华病理学杂志2013，42，4： | 5 |
| 9 | Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast显示文摘Current yeast metabolic engineering in isoprenoids production mainly focuses on rewiring of cytosolic metabolic pathway.However,the precursors,cofactors and the enzymes are distributed in various sub-cellular compartments,which may hamper isoprenoid biosynthesis.On the other side,pathway compartmentalization provides several advantages for improving metabolic flux toward target products.We here summarize the recent advances on harnessing sub-organelle for isoprenoids biosynthesis in yeast,and analyze the knowledge about the localization of enzymes,cofactors and metabolites for guiding the rewiring of the sub-organelle metabolism.This review may provide some insights for constructing efficient yeast cell factories for production of isoprenoids and even other natural products. | Xuan Cao Shan Yang Chunyang Cao Yongjin J.Zhou | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 5 |
| 10 | Amylase Production by the Marine Yeast Aureobasidium pullulans N13d显示文摘The marine yeast strain N13d, producing an extracellular amylase, was isolated from the deep sea sediments of the Pa-cific Ocean. This strain was identified to be Aureobasidium pullulans by 18S rRNA gene sequence analysis and routine yeast identi-fication methods. The optimal sea water medium for amylase production by this yeast strain was 1.0% peptone and 1.0% soluble starch with pH 4.0. The optimal conditions for amylase production by this yeast strain were with temperature 28 ℃, aeration rate 6 Lmin-1 and agitation speed 250 rmin-1. Under these conditions, 58.5 units of amylase activity per mg protein were produced within 56 h of fermentation. | LI Haifeng, CHI Zhenming *, WANG Xiaohong, and MA Chunling UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 5 |
| 11 | Understanding the endocrine disruption of chiral pesticides:The enantioselectivity in estrogenic activity of synthetic pyrethroids显示文摘Synthetic pyrethroids(SPs) ,a family of chiral insecticides consisting of multiple stereoismers,have been regarded as estrogenic endocrine-disrupting chemicals(EDCs) .In this study,we applied the yeast two-hybrid and molecular docking(MD) assay to assess the enantioselective estrogenic activities of three commonly used SPs,bifenthrin(cis-BF) ,permethrin(PM) and fenvalerate(Fen) .The β-galactosidase analyses indicated that all of the testing pyrethroids displayed significant(p<0.05) enantioselectivity.The results showed that the estrogenic potential of cis-BF was mainly attributed to 1S-cis-BF.Neither PM nor Fen showed estrogenic effects.However,two stereoisomers of PM possessed estrogenic potential activities.αR-2R-Fen and αS-2S-Fen also induced the β-galactosidase activity.The inability to initiate the reporter gene expression by the racemic chemicals may be due to the low ratios of these isomers or the antagonism among them.The strong hydrophobic interaction and the hydrogen bond between positive estrogenic isomers and ERα support our biological testing results.This research demonstrated that the enantioselective estrogenic activity of chiral SPs was due to selective binding between their isomers and the ERαreceptor.The data suggests that enantioselectivity of these chiral pesticides is significant to their estrogenic activities. | WANG Cui 1,ZHANG Quan 1,ZHANG XiaoFeng 1,LIU Jing 2 & LIU WeiPing 2 1College of Biological and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310032,China 2Key Laboratory of Environmental Remediation and Ecosystem Health,Ministry of Education College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China | 2010 | Science China Chemistry2010，53，5： | 4 |
| 12 | Improvement in affinity and thermostability of a fully human antibody against interleukin-17A by yeast-display technology and CDR grafting显示文摘Monoclonal antibodies(m Abs) are widely used in many fields due to their high specificity and ability to recognize a broad range of antigens. IL-17 A can induce a rapid inflammatory response both alone and synergistically with other proinflammatory cytokines. Accumulating evidence suggests that therapeutic intervention of IL-17 A signaling offers an attractive treatment option for autoimmune diseases and cancer. Here, we present a combinatorial approach for optimizing the affinity and thermostability of a novel anti-h IL-17 A antibody. From a large na?ve phage-displayed library, we isolated the anti-IL-17 A m Ab 7 H9 that can neutralize the effects of recombinant human IL-17 A. However, the modest neutralization potency and poor thermostability limit its therapeutic applications. In vitro affinity optimization was then used to generate 8 D3 by using yeast-displayed random mutagenesis libraries.This resulted in four key amino acid changes and provided an approximately 15-fold potency increase in a cell-based neutralization assay. Complementarity-determining regions(CDRs) of 8 D3 were further grafted onto the stable framework of the hu Fv 4 D5 to improve thermostability. The resulting hybrid antibody9 NT/S has superior stabilization and affinities beyond its original antibody. Human fibrosarcoma cellbased assays and in vivo analyses in mice indicated that the anti-IL-17 A antibody 9 NT/S efficiently inhibited the secretion of IL-17 A-induced proinflammatory cytokines. Therefore, this lead anti-IL-17 A m Ab might be used as a potential best-in-class candidate for treating IL-17 A related diseases. | Wei Sun Zhaona Yang Heng Lin Ming Liu Chenxi Zhao Xueying Hou Zhuowei Hu Bing Cuin | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 4 |
| 13 | Loss of heterozygosity by SCRaMbLEing显示文摘Genetic variation drives phenotypic evolution within populations. Genetic variation can be divided into different forms according to the size of genomic changes. However, study of large-scale genomic variation such as structural variation and aneuploidy is still limited and mainly based on the static, predetermined feature of individual genomes. Here, using SCRaMbLE,different levels of loss of heterozygosity(LOH) events including short-range LOH, long-range LOH and whole chromosome LOH were detected in evolved strains. By contrast, using rapid adaptive evolution, aneuploidy was detected in the adaptive strains. It was further found that deletion of gene GLN3, long-range LOH in the left arm of synthetic chromosome Ⅹ, whole chromosome LOH of synthetic chromosome Ⅹ, and duplication of chromosome Ⅷ(trisomy) lead to increased rapamycin resistance in synthetic yeast. Comparative analysis of genome stability of evolved strains indicates that the aneuploid strain has a higher frequency of degeneration than the SCRaMbLEd strain. These findings enrich our understanding of genetic mechanism of rapamycin resistance in yeast, and provide valuable insights into yeast genome architecture and function. | Yunxiang Li Yi Wu Lu Ma Zhou Guo Wenhai Xiao Yingjin Yuan | 2019 | Science China(Life Sciences)2019，62，3： | 4 |
| 14 | 以分子生物学鉴定结果为“金标准”评估Rapid ID Yeast Plus及API20C AUX对酵母样真菌的鉴定效能显示文摘目的以分子生物学方法为'金标准'对两种商品化酵母样真菌鉴定产品Rapid ID Yeast Plus(简称RapID YST)及API20C AUX(简称API20C)的鉴定效能进行评估。方法从2010年中国医院侵袭性真菌感染监测网菌株库中筛选酵母样真菌25种,共计194株。其中,包含临床最常见的5种酵母样真菌(白念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌、新生隐球菌)共130株,占研究总菌株数的67.0%。所有菌株已经过分子生物学方法准确鉴定至种水平。菌株复苏分纯后,严格按照产品操作指南,平行进行RapID YST和API20C鉴定。结果所研究菌株中,有181株(18种)在RapID YST鉴定菌种数据库中,所有在库菌株种及复合体鉴定正确率为87.8%(159/181)。相比,API鉴定菌种库包含菌株174株(18种),在库菌株种及复合体鉴定正确率为92.0%(160/174)。RapID YST与API20C对于5种临床常见的酵母样真菌的种鉴定正确率分别为93.1%和97.1%。对于库外菌株,RapID YST的鉴定错误率分别为23.1%(3/13),相比API20C的鉴定错误率为60.0%(12/20)。综合此次评估结果,二者对酵母样真菌的鉴定效能无显著差异(McNemar检验,P>0.05)。结论两种商品化产品对酵母样真菌的鉴定效能基本一致;相较而言,RapID YST在操作便捷性、检测时间方面具有较大优势。 | 范欣 王贺 苏霄翔 肖盟 马雪菲 马超越 刘旭光 马然 吴玥 陈艳钊 徐英春 | 2013 | 中国真菌学杂志2013，8，5： | 4 |
| 15 | Two novel eukaryotic translation initiation factor 5A genes from Populus simonii×P.nigra confer tolerance to abiotic stresses in Saccharomyces cerevisiae显示文摘The role of plant e IF5 A proteins in multiple biological processes, such as protein synthesis regulation,translation elongation, m RNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized Psne IF5A2 and Psne IF5A4 from young poplar(P. simonii 9 P. nigra) leaves. The deduced amino acid sequences of Psne IF5A2 and Psne IF5A4 were 98 %similar to each other, and they are orthologs of e IF5A1 in Arabidopsis. In a subcellular localization analysis,Psne IF5A2 and Psne IF5A4 proteins were localized in the nucleus and cytoplasm. q RT-PCR analysis showed that Psne IF5A2 and Psne IF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing Psne IF5A2 and Psne IF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that Psne IF5A2 and Psne IF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants. | Tangchun Zheng Lina Zang Lijuan Dai Chuanping Yang Guanzheng Qu | 2017 | Journal of Forestry Research2017，28，3： | 4 |
| 16 | Response of primiparous and multiparous buffaloes to yeast culture supplementation during early and mid-lactation显示文摘Strains of live Saccharomyces cerevisiae yeast have exhibited probiotic effects in ruminants. This study investigated the effects of the dietary yeast supplement, S. cerevisiae(Yea-Sacc^(1026)), on primiparous(PP)and multiparous(MP) Egyptian buffaloes in early to mid-lactation. Lactating buffaloes were fed either a basal total mixed ration(TMR, control; 4 PP and 8 MP) or the basal TMR plus 10 g Yea-Sacc^(1026) per buffalo cow per day(yeast; 4 PP and 8 MP). The feeds were given from 15 days prepartum to 180 days postpartum. Feed intake, body weight, and milk yields(MY) were recorded, and milk and blood samples were collected for analyses. Feces were collected from days 45 to 47 during early lactation and from days 90 to92 during mid-lactation to determine apparent digestibility of dry matter(DM), organic matter(OM),crude protein(CP) and crude fiber(CF). Energy corrected milk yield(ECM), feed conversion, and energy and nitrogen conversion efficiency were calculated. Yeast treated MP buffaloes consumed more DM(P < 0.041) and CP than the untreated control group. Apparent digestibility of DM and OM were significantly greater at mid-lactation for treated versus control group(P = 0.001). Crude fiber digestibility was greater in MP than in PP buffaloes(P = 0.049), and yeast supplemented MP cows had a greater CF digestibility than control MP buffaloes at mid-lactation(P = 0.010). Total blood lipids decreased after yeast supplementation(P= 0.029). Milk yields, ECM, fat and protein yields increased for yeast treated MP buffaloes(P < 0.039). The study concluded that the response to yeast supplementation in buffalo cows is parity dependent. Multiparous buffaloes respond to yeast supplementation with an increased DM intake and CF digestibility without significant weight gains, allowing a greater ECM yield with less fat mobilization. Supplementing buffaloes with yeast culture may increase milk production in early lactation and results in a more persistent milk production during mid-lactation. Feed conversion and energy and nitrogen conversion efficiency may be increased with the use of yeast supplementation in Egyptian buffaloes. | Hanne H.Hansen Nasr E.El-Bordeny Hossam M.Ebeid | 2017 | Animal Nutrition2017，，4： | 4 |
| 17 | Six new monacolin analogs from red yeast rice显示文摘Six novel monacolin analogs, monacolins V_1–V_6(1–6), together with seven known ones(7–13), were isolated from the ethyl acetate extract of red yeast rice. Their structures and absolute configurations were determined by spectroscopic methods,especially 2D NMR(~1H-~1HCOSY, HSQC, HMBC, and NOESY/ROESY) and CD spectroscopic analyses as well as chemical derivation.Monacolins V_2(2) and V_3(3) represent the first examples of monacolins with 3-hydroxybutyrate substitute. The anti-inflammatory inhibitory activities against the lipopolysaccharide(LPS) induced NO production in BV-2 cells as well as antioxidant activities against rat liver microsomal lipid peroxidation were evaluated. | LIU Bing-Yu XU Fei BAI Jian YAN Dao-Jiang ZHANG Le ZHANG Dan HU You-Cai | 2019 | Chinese Journal of Natural Medicines2019，17，5： | 3 |
| 18 | Isolation and characterization of a human apoptosis-inducing gene with yeast two-hybrid system显示文摘asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism of asy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7 kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts of asyip gene contain common 5’ end and different 3’ end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction | 齐兵 齐义鹏 Masuo Yutsudo 刘青珍 | 2000 | Science China(Life Sciences)2000，43，3： | 3 |
| 19 | Predominant yeasts in Chinese Dong fermented pork(Nanx Wudl)and their aroma-producing properties in fermented sausage condition显示文摘Yeasts are one of the predominant microbial groups in fermented meats.In this study,yeast communities of Chinese Dong fermented pork(Nanx Wudl)were investigated and the technological properties of 73 yeast isolates were evaluated.Through culture-dependent and high-throughput sequencing methods,the main yeast species identified included Pichia membranifaciens,Kazachstania bulderi,Millerozyma farinosa,Candida zeylanoides,Kazachstania exigua,Candida parapsilosis and Saccharomyces cerevisiae.Among these yeasts,P.membranifaciens,M.farinosa,K.exigua and K.bulderi were detected in fermented meats for the first time.A total of 73 yeast isolates was investigated for their lipolytic and proteolytic activities.All yeast species showed lipolytic activity,while proteolytic activity against meat protein was only detected in S.cerevisiae.Assay of aroma-producing potential was performed in a model simulating fermented sausage condition.Inoculation of yeast strains increased volatiles production,especially esters and alcohols.The highest ester production was observed in S.cerevisiae Y70 strain,followed by K.exigua Y12 and K.bulderi Y19.C.zeylanoides Y10 and S.cerevisiae Y70 were the highest producers of benzeneethanol and 3-methyl-1-butanol.S.cerevisiae Y70 with its highest production of branched alcohols and esters could be a promising candidate as aroma enhancer in the manufacture of fermented sausages. | Ruifang Mi Xi Chen Suyue Xiong Biao Qi Jiapeng Li Xiaoling Qiao Wenhua Chen Chao Qu Shouwei Wang | 2021 | Food Science and Human Wellness2021，10，2： | 3 |
| 20 | CLONING AND CHARACTERIZATION OF FRAGMENTS RELATED TO PROLINE SYNTHESIS AND THEIR EFFECT ON HOST CELLS显示文摘Complementation of mutation in E. coli has been successful in cloning specific eukaryotic genes of yeast and animal cells for use in transformation. As a metabolic pathway of housekeeping protein, the synthesis of proline and its regulation might be conservative in prokaryotic and eukaryotic cells. With this in mind, we had cloned pro^+ genes from mouse and frog using this method in 1980. | 陈受宜 王阁 劳为德 | 1988 | Chinese Science Bulletin1988，33，10： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员