由有胚胎的干细胞的熔化的 Reprogramming 体的房间不在老鼠引起 Dlk1-Dio3 区域的 silencing

查看全文 作　　者：Nariman R Battulin;Anna A Khabarova;Ul’yana A Boyarskikh;Aleksey G Menzorov;Maxim L Filipenko;Oleg L Serov

机构地区：Institute of Cytology and Genetics SD RAS, Lavrentyeva 10, Novosibirsk 630090, Russian;Institute of Chemical Biology and Basic Medicine SD RAS, Lavrentyeva 8, Novosibirsk 630090, Russian

出　　处：《World Journal of Stem Cells》索引2012年第4卷第8期,共7页

基　　金：Supported by Grant from the Russian Academy of Sciences,Siberian Branch N48 摘　　要：AIM:To examine the imprinted Dlk1-Dio3 locus in pluripotent embryonic stem(ES)cell/fibroblast hybrid cells.METHODS:Gtl2,Rian,and Mirg mRNA expression in mouse pluripotent ES cell/fibroblast hybrid cells was examined by real-time reverse transcription-polymerase chain reaction.Pyrosequencing and bisulfate sequencing were used to determine the DNA methylation level of the Dlk1-Dio3 locus imprinting control region. RESULTS:The selected hybrid clones had a near-tetraploid karyotype and were highly pluripotent judging from their capacity to generate chimeric embryos and adult chimeras.Our data clearly demonstrate that Gtl2,Rian,and Mirg,which are imprinted genes within the Dlk1-Dio3 locus,are active in all examined ES cell/fibroblast hybrid clones.In spite of interclonal variability,the expression of the imprinted genes is comparable to that of ES cells and fibroblasts.Quantitative analysis of the DNA methylation status of the intergenic differentially methylated region(IG DMR)within the Dlk1-Dio3 locus by pyrosequencing and bisulfite sequencing clearly showed that the DNA methylation status of the imprinted region in the tested hybrid clones was comparable to that of both ES cells and fibroblasts.CONCLUSION:Reprogramming process in a hybrid cell system is achieved without marked alteration of the imprinted Dlk1-Dio3 locus. 关键词：Cell FUSION EMBRYONIC stem cells REPROGRAMMING PLURIPOTENCY Imprinted Dlk1-Dio3 region DNA METHYLATION

相关文献

参考文献（23）
引证文献（0）
耦合文献（10）

参考文献(23)

耦合文献(10)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

由有胚胎的干细胞的熔化的 Reprogramming 体的房间不在老鼠引起 Dlk1-Dio3 区域的 silencing

参考文献(23)

耦合文献(10)

用户提示