Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus

查看全文 作　　者：Kilani-Jaziri [1,2]Soumaya;Ghedira [1,2]Zied;Nasr [1,2]Nouha;Krifa [1,2]Mounira;Ghedira [1]Kamel;Franca Dijoux Marie [3,4]Genviève;Ghedira Chekir [2]Leila

机构地区：[1]Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12.Universite de Monastic,Faculte de Pharmacie de Monastir;[2]Laboratoire de Biologie Moleculaire et Cellulaire,Faculté de Medecine Dentaire de Monastir,Universite de Monastir,Rue Aricenne,5019 Monastir,Tunisie;[3]Laboratoire de Botanique.Pharmacognosie et Phytothérapie,UMR CNRS 5557 Ecologie Microbienne;[4]Institut des Sciences Pharmaceutiques et Biologiques-Faculte de Pharmacie Université Claude Bernard,Btiment Netien-8 Avenue Rockefeller-69373 Lyon cedex 08,France

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2014年第7卷第2期,共8页

基　　金：the 'Ministere Francais des Affaires Etrangeres'(Action Integree de Cooperation Inter universitaire Franco-Tunisienne,PHC UTIQUE 07 G0836 PAR);the Ministry of Higher Education.Scientific Research and Technology in Tunisia for the financial assistance 摘　　要：Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for their cytotoxic and apoptotic activities.The major compound of the methanol extract was isolated.Both metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system.However,16,8,and 4 mg/mL of each extract were tested to investigate their OH.formation scavenging potential.Aqueous extract(800,400.and 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H_2O_2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell lines.The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic analysis.Results:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase activity.Vet.the same extracts inhibited lipid peroxidation by 61.5%and 42.0%.respectively.Roth extracts inhibited OH.formation by 27.1%and 25.3%,respectively.Only methanol cxtract induced DNA degradation.Orientin was determined as the major compound isolated from the butanol fraction of metlianol extract.Conclusions:It appears that C.rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer. 关键词：Polyphenols ORIENTIN Xanthine/Xanthine OXIDASE ASSAY Lipid PEROXIDATION ASSAY DEOXYRIBOSE ASSAY DNA fragmentation

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Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus

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