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Evaluation of extracts and essential oil from Callistemon viminalis leaves:Antibacterial and antioxidant activities,total phenolic and flavonoid contents

查看全文 作  者:Mohamed ZM [1]Salem;Hayssam M [2,3]Ali;Nader A El-[4]Shanhorey;Ahmed Abdel-[2,5]Megeed 高影响力作者 机构地区:[1]Forestry and Wood Technology Department,Faculty of Agriculture(EL-Shatby),Alexandria University;[2]Botany and Microbiology Department,College of Science,King Saud University;[3]Timber Trees Research Department,Sabahia Horticulture Research Station,Horticulture Research Institute,Agriculture Research Center;[4]Department of Botanical Gardens Research,Horticultural Research Institute(ARC);[5]Faculty of Agriculture(Saba Basha),Alexandria University高影响力机构 出  处:《Asian Pacific Journal of Tropical Medicine》索引2013年第6卷第10期,共7页高影响力期刊 基  金:funded by the Deanship of Scientific Research at King Saud University with the research group project No.RGP-VPP-010 摘  要:Objective:To investigate antioxidant and antibacterial activities of Callistemon viminalis(C.viminalis)leaves.Methods:The essential oil of C.viminalis leaves obtained by hydrodistillation was analyzed by GC/MS.Different extracts were tested for total phenolic and flavonoid contents and in vitro antioxidant(DPPH assay)and antibacterial(agar disc diffusion and 96-well micro-plates methods)actives.Results:Fourteen components were identified in the essential oil,representing 98.94%of the total oil.The major components were 1,8-cineole(64.53%)andα-pinene(9.69%).Leaf essential oil exhibited the highest antioxidant activity of(88.60±1.51)%comparable to gallic acid,a standard compound[(80.00±2.12)%].Additionally,the biggest zone of inhibitions against the studied bacterial strains was observed by the essential oil when compared to the standard antibiotic(tetracycline).The crude methanol extract and ethyl acetate fraction had a significant antibacterial activity against the tested bacterial strains.Conclusions:It can be suggested that C.viminalis is a great potential source of antibacterial and antioxidant compounds useful for new antimicrobial drugs from the natural basis.The present study revealed that the essential oil as well as the methanol extracts and ethyl acetate fraction of C.viminalis leaves exhibited highly significant antibacterial activity against the tested bacterial strains. 关 键 词:Callistemon viminalis Essential oil ANTIBACTERIAL ACTIVITY ANTIOXIDANT ACTIVITY PHENOLIC CONTENT Flavonoid CONTENT
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