Tissue array for Tp53,C-myc,CCND1 gene over-expression in different tumors

查看全文 作　　者：Guo-Yan [1]Liu;Qi [2]Luo;Bin [3]Xiong;Chao [2]Pan;Ping [2]Yin;Hong-Feng [2]Liao;Wei-Chun [2]Zhuang;Hong-Zhi [4]Gao

机构地区：[1]Zhongnan Hospital, Wuhan University,Wuhan 430071, China; The General Surgery of the AffiliatedZhongshan Hospital of Xiamen University, The Digest DiseaseResearch Institution of Xiamen University, Xiamen 361004,Fujian Province, China;[2]Department of GeneralHong-Feng Liao, Wei-ChunSurgery, Affiliated ZhongshanHospital of Xiamen University; Digest Disease ResearchInstitute of Xiamen University, Xiamen 361004, FujianProvince. China;[3]Zhongnan Hospital, Wuhan University, Wuhan430071, Hubei Proyince, China;[4]The Second Affiliated Hospital of FujianMedical University, Fujian Province, China

出　　处：《World Journal of Gastroenterology》索引2008年第14卷第47期,共9页

基　　金：Supported by Foundation of Xiamen Science and Technology Bureau,Fujian Province,China,No.3502Z20074023 摘　　要：AIM:To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53,C-myc,and CCND1 genes in development of tumors in human organs and their adjacent normal tissues,as well as the possible relation between well-and poorly-differentiated tumors.METHODS:A tissue array consisting of seven different tumors was generated.The tissue array included 120 points of esophagus,120 points of stomach,80 points of rectum,60 points of thyroid gland,100 points of mammary gland,80 points of liver,and 80 points of colon.Expressions of Tp53,C-myc,and CCND1 were determined by RNA in situ hybridization.3' terminal digoxin-labeled anti-sense single stranded oligonucleotide and locked nucleic acid modifying probe were used.RESULTS:The expression level of Tp53 gene was higher in six different carcinoma tissue samples than in paracancerous tissue samples with the exception in colon carcinoma tissue samples(P < 0.05).The expression level of CCND1 gene was significantly different in different carcinoma tissue samples with the exception in esophagus and colon carcinoma tissue samples.The expression level of C-myc gene was different in esophagus carcinoma tissue samples(c2 = 18.495,P = 0.000),stomach carcinoma tissue samples(c2 = 23.750,P = 0.000),and thyroid gland tissue samples(c2 = 10.999,P = 0.004).The intensity of signals was also different in different carcinoma tissue samples and paracancerous tissue samples.CONCLUSION:Over-expression of the Tp53,CCND1,and C-myc genes appears to play a role in development of human cancer by regulating the expression of mRNA.Tp53,CCND1 and C-myc genes are significantly correlated with the development of different carcinomas. 关键词：TP53 C-MYC CCND1 RNA 基因表达肿瘤学

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Tissue array for Tp53,C-myc,CCND1 gene over-expression in different tumors

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