SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin

查看全文 作　　者：ZHANG [1,2]ZhaoXia;ZHAO [1]HongWei;ZHU [1,2]HongPing;GE [1,2]Min;WANG [1]WenFeng;YAO [1]SiDe;LI [1]WenXin

机构地区：[1]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China;[2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China Chemistry》索引2007年第50卷第1期,共7页

基　　金：the National Natural Science Foundation of China(Grant Nos.20373086 and 10574134);the research program of the Chinese Academy ofSciences 摘　　要：The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Indica- tions showed that the mechanisms and products of oxidative damage were relative to the concentra- tion of riboflavin,the time of irradiation and the ambience.The type I process was examined in a nitrogen saturated solution,whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process.The study also suggested that antioxidants,such as melatonin, can reduce the damage of lysozyme effectively. 关键词：LYSOZYME RIBOFLAVIN PHOTOOXIDATION SDS-PAGE ANTIOXIDANT

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SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin

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