A novel tobacco gene coding for a product similar to bacterial two-component regulators

查看全文 作　　者：Jinsong [1]Zhang;Can [1]Xie;Feng [1]Liu;Fenghua [1]Liu;Shouyi [1]Chen 机构地区：[1]Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China 出　　处：《Chinese Science Bulletin》索引1999年第44卷第11期,共5页 摘　　要：The two-component signaling system has been studied in bacteria. It takes part in signal transduction of adaptive behavior. Recent studies have shown that a similar two-component system is also present in eukaryotes. Examples of this are ETR1 and CKI1 genes which may involve the signal transduction of plant hormone ethylene and cytokinin respectively. The cloning and characterization of a novel gene ( NTHK1 ) fragment from tobacco are presented.Its partial sequence codes for a product which shows similarity to many two-component signaling proteins. Southern blot analysis indicated that there are 2 to 3 copies of NTHK1 gene in tobacco genome (allotetraploid). Homologous genes may also exist in other plants such as Arabidopsis, soybean and spinach. The expression of NTHK1 gene has also been analyzed in tobacco.Further studies on the isolation of full-length cDNA of NTHK1 gene will elucidate more clearly its function in signal perception and transduction. 关 键 词：TOBACCO TWO-COMPONENT SIGNALING system signal transduction.