Rapid and efficient method for isolating plant high molecular weight(HMW)DNA with high purity

查看全文 作　　者：Fang [1]Qiu;Jianmin [1]Fu;Weiwu [1]Xie;Chuanyou [1]Li;Bin [1]Wang 机构地区：[1]Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China 出　　处：《Chinese Science Bulletin》索引1999年第44卷第4期,共3页 摘　　要：PREPARATION of HMW DNA (Megabase-size) is the basis for construction of genomic library with large DNA inserts such as bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC), and for long-range physical mapping. It can also be used for the macro-study of repeat sequences. Since HMW DNA during preparation is inclined to be sheared physically and digested by internal nucleases, it is very difficult to prepare the HMW DNA. Initially, plant HMW DNA was prepared by embedding protoplasts in the low melting-point (LMP) agarose; however, it had several disadvantages: (ⅰ) Culture of protoplasts was time-consuming, costly and tedious. ( ⅱ ) It was only used successfully for limited 关 键 词：DNA Rapid and efficient method for isolating plant high molecular weight HIGH