Engagement of circular RNA HECW2 in non-autophagic role of ATG5 implicated in endothelial-mesenchymal transition

查看全文 作　　者：YANG [1]Li;HAN [1]Bing;ZHANG [1]Yuan;BAI [1]Ying;CHAO [2]Jie;HU [3]Gang;YAO Hong-[1,4]hong 机构地区：[1]Department of PharmacologySchool of Medicine, Southeast University, Nanjing 210009, China;[2]Department of Physiology, School of Medicine, Southeast University, Nanjing 210009, China;[3]Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmac 出　　处：《中国药理学与毒理学杂志》索引2018年第32卷第9期,共2页 摘　　要：Endothelial-mesenchymal transition(EndoMT) is associated with damage to blood-brain barrier(BBB) integrity.Circular RNAs(circRNAs) are highly expressed in the brain and are involved in brain diseases;however,whether circR NAs regulate the EndoM T in the brain remains unknown.Our study demonstrated that circH ECW2 regulated the EndoM T by directly binding to MIR30 D,a significantly downregulated miR NA from miR NA profiling,which subsequently caused an increased expression of ATG5.These findings shed new light on the understanding of the noncanonical role of ATG5 in the EndoMT induced by methamphetamine or lipopolysaccharide.The in vivo relevance was confirmed as microinjection of circHecw2 siRNA lentivirus into the mouse hippocampus suppressed the EndoMT induced by LPS.These findings provide novel insights regarding the contribution of circHECW2 to the nonautophagic role of ATG5 in the EndoMT process in the context of drug abuse and the broad range of neuroinflammatory disorders. 关 键 词：ATG5 CIRCULAR RNA HECW2 EndoMT MIR30D nonautophagy