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Detection of H.pylori DNA in gastric epithelial cells by in situ hybridization

查看全文 作　　者：Xin-Liang Lu Ke-Da Oian Xun-Qiu Tang Yong-Liang Zhu Qin Du,Department of Digestive Diseases,Second Affiliated Hospital,Zhejiang University Medical College,Hangzhou 310009,Zhejiang Province,China 高影响力作者 出　　处：《World Journal of Gastroenterology》索引2002年第8卷第2期,共3页高影响力期刊 摘　　要：AIM: To investigate the presence of H. pylori DNA withingastric epithelial cells in patients with H. pylori infection andits possible carcinogenic mechanism.METHODS: Total 112 patients, with pathologically confirmedchronic superficial gastritis, chronic atrophic gastritis,intestinal metaplasia, atypical hyperplasia or gastrio cancerwere studied .Among them, 28 were H. pylori negative and84 H. pylori positive. H. pylori DNA in gastric epithelialcells was detected by GenPoint catalyzed signalamplification system for in situ hybridization.RESULTS: In the H. pylori positive group, zero out of 24chronic superficial gastritis (0. 0 %), four out of 25precancerous changes (16.0 %) and thirteen out of 35gastric cancers (37. 1 %) showed H. pylori DNA in thenucleus of gastric epithelial cells, the positive rates of H.pylori DNA in the nucleus of gastric epithelial cells wereprogressively inoreased in chronic superficial gastritis,precancerous changes and gastric cancer groups (χ2 = 12.56, P = 0. 002); One out of 24 ohronic superficial gastritis(4.2 %), eleven out of 25 precancerous ohangas (44.0 %)and thirteen out of 35 gastric cancers (37. 1 %) showed H.pylori DNA in the cytoplasm of gastric epithelial cells (χ2 =10.86, P = 0.004). In the H. pylorinegative group, only onepatient with gastric cancer was found H. pylori DNA in thenucleus of gastric epithelial cells; Only two patients, onepatient with precancerous changes and another with gastriccancer, showed H. pylori DNA in the cytoplasm of gastricepithelial calls. Furthermore, H. pylori DNA must have been inthe ayteplasm as long as it existed in the nucleus of gastricepithelial cells.CONCLUSION: H. pyloriDNA exists both in the nucleus andthe cytoplasm of gastric epithelial cells in patients with H.pylori infections. The pathological progression from chronicsuperficial gastritis, precancerous changes to gastric canceris associated with higher positive rates of H. pylori DNApresence in the nucleus of gastric epithelial cells. 关 键 词：胃上皮细胞 幽门螺杆菌 DNA 检测 原位杂交
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