Pathways related to PMA-differentiated THP1 human monocytic leukemia cells revealed by RNA-Seq

查看全文 作　　者：ZENG [1,2,3]ChengWu;WANG [4]WenTao;YU [3]XiBao;YANG [2]LiJian;CHEN [2]ShaoHua;LI [1,2,3]YangQiu

机构地区：[1]First Affiliated Hospital, Jinan University;[2]Institute of Hematology, Medical College, Jinan University;[3]Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University;[4]Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen University

出　　处：《Science China(Life Sciences)》索引2015年第58卷第12期,共6页

基　　金：supported by funds from the National Natural Science Foundation of China(81400102);the Chinese Postdoctoral Science Foundation(2015M570751);the National Undergraduate Training Program for Innovation and Entrepreneurship(201510559043);the Medical Scientific Research Foundation of Guangdong Province,China(A2015420) 摘　　要：Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12-myristate 13-acetate(PMA). However, little is known about the mechanism responsible for regulating this differentiation process. Here, we performed high-throughput RNA-Seq analysis to investigate the genes differently expressed in THP1 cells treated with and without PMA and examined those that may be responsible for the PMA-induced differentiation of monocytes into macrophages. We found 3,000 genes to be differentially expressed after PMA treatment. Gene ontology analysis revealed that genes related to cellular processes and regulation of biological processes were significantly enriched. KEGG analysis also demonstrated that the differentially expressed genes(DEGs) were significantly enriched in the PI3K/AKT signaling pathway and phagosome pathway. Importantly, we reveal an important role of the PI3K/AKT pathway in PMA-induced THP1 cell differentiation. The identified DEGs and pathways may facilitate further study of the detailed molecular mechanisms of THP1 differentiation. Thus, our results provide numerous potential therapeutic targets for modulation of the differentiation of this disease. 关键词：白血病细胞分化过程单核细胞 PMA RNA 显示差异表达基因信号通路

相关文献

参考文献（26）
引证文献（8）
耦合文献（33）

参考文献(26)

引证文献(8)

耦合文献(33)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Pathways related to PMA-differentiated THP1 human monocytic leukemia cells revealed by RNA-Seq

参考文献(26)

引证文献(8)

耦合文献(33)

用户提示