Expression of lens-related microRNAs in transparent infant lenses and congenital cataract

查看全文 作　　者：Chang-Rui [1]Wu;Min [2]Ye;Li [1]Qin;Yue [3]Yin;Cheng [1]Pei

机构地区：[1]Department of Ophthalmology,the First Affiliated Hospital of Xi’an Jiaotong University;[2]Ningxia Eye Hospital,People Hospital of Ningxia Hui Autonomous Region(First Affiliated Hospital of Northwest University for Nationalities);[3]Basic Research Center,Affiliated Shaanxi Provincial Tumor Hospital,College of Medicine,Xi’an Jiaotong University

出　　处：《International Journal of Ophthalmology(English edition)》索引2017年第10卷第3期,共5页

基　　金：Supported by the Natural Science Foundation of China(No.81470614);the Fundamental Research Funds for the Central Universities sponsored by Xi’an Jiaotong University(No.xjj2013067);Youth Foundation of the First Affiliated Hospital,Medical College,Xi’an Jiaotong University(No.2014YK7);Scientific Research Funds for the Health and Family Planning of Shaanxi Province(No.2016D068) 摘　　要：AIM:To identify the expression of lens-related micro RNAs(miRNAs)in the central epithelium of transparent infant lenses and congenital cataract. METHODS:Lens-related mi RNAs were retrieved from Pub Med database. The expression levels of these mi RNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction(RT-PCR). mi Randa algorithm was used to predict the target genes of these differentially expressed mi RNAs. The target m RNA was validated.RESULTS:Six lens-related mi RNAs were retrieved from screening Pub Med database. The most abundant mi RNA in transparent infant lenses according to stem-loop RT-PCR was mi R-184. miR-182 was up-regulated in congenital cataract. Contrarily,miR-204 and miR-124 was down-regulated.mi R-204 exhibited a more significant decrease in expression than mi R-124. In addition,Meis2 was predicted to be the target of mi R-204 using mi Randa algorithm. mi R-204mimic/antagomir transfection experiments suggested the negative correlation between the expression of mi R-204 and Meis2.CONCLUSION:The expression levels of miR-182,miR-204 and mi R-124 differ between the central epithelium of transparent infant lens and congenital cataract,suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act via silencing Meis2 to regulate lens development and congenital cataract formation. 关键词：透镜相关的 miRNAs 先天的奔流 miR-204 Meis2

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Expression of lens-related microRNAs in transparent infant lenses and congenital cataract

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