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Anti-fibrotic Effects of Salvia miltiorrhiza and Ligustrazine Injection on LX-2 Cells Involved with Increased N-myc Downstream-Regulated Gene 2 Expression

查看全文 作　　者：zheng [1,2]jin;ma li-[2]tian;ren qin-[2]you;hu [3]yue;bai [4]yang;bian [5]huan;zhang [2]yi;zhou yong-[6]chun;yang ming-[1]hui 高影响力作者 机构地区：[1]department of traditional chinese medicine,chinese pla general hospital,Beijing 100853,China;[2]department of integrated traditional and western medicine of oncology,tangdu hospital,the fourth military medical university,Xi'an 710032,China;[3]department of gastroenterology,bethune international peace hospital,Shijiazhuang 050082,China;[4]department of anatomy and k.k.leung brain research center,faculty of basic medicine,the fourth military medical university;[5]department of biochemistry and molecular biology,the fourth military medical university,Xi'an 710032,China;[6]department of radiotherapy oncology,the first affiliated hospital,the fourth military medical university,Xi'an 710032,China高影响力机构 出　　处：《Chinese Journal of Integrative Medicine》索引2017年第23卷第12期,共6页高影响力期刊 基　　金：Supported by the National Natural Science Foundation of China(No.81272490 and 81100764) 摘　　要：Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection(SML) on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2(NDRG2, a tumor suppressor gene). Methods: HSCs from the LX-2 cell line were cultured in vitro. The proliferative state of different initial LX-2 cell numbers was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) colorimetric assay. LX-2 cells were plated in 96-well plates at an approximate density of 2.50×10~4 cells/mL and cultured for 24 h followed by the application of different concentrations of SML(1, 2, 4 and 8 μL/mL). Cell proliferation was measured using the MTT assay at 24 and 48 h. Apoptosis was detected by flow cytometry at 24 h. LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer. The levels of NDRG2 and β-catenin were measured by Western blot. Results: With the exception of the 1 and 2 μL/mL concentrations, 4 and 8 μL/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h(P<0.05). With the exception of the 1 and 2 μL/mL concentrations, the NDRG2 expression level was greatly increased in a concentration-dependent manner. However, the level of β-catenin was unaffected. Conclusion: SML inhibit LX-2 cell proliferation in a concentration-dependent manner, and the mechanism may be associated with NDRG2 over-expression. 关 键 词：SALVIA miltiorrhiza and LIGUSTRAZINE Injection, N-MYC downstream-regulated gene 2, hepaticstellate cell, proliferation, apoptosis
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